Fmoc-FFC
Fmoc-FFC is a competitive MBL (NDM-1, VIM-2, and IMP-1) inhibitor with Ki values of 2.3, 6.0, and 7.1 μM, respectively. Fmoc-FFC alone has no antibacterial activity, but acts synergistically with Meropenem (HY-13678) to restore antibacterial activity against MBL/NDM-1-producing E. coli. Fmoc-FFC supports cell-cell adhesion and can serve as a scaffold for cell culture growth. Fmoc-FFC can be used in research on systemic infections caused by NDM-1-producing E. coli.
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- Fòrmula: C36H35N3O5S
- Peso molecular:621.75
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
In Vitro
Fmoc-FFC (1-10 μM; 5 min) competitively inhibits NDM-1 independently of zinc deprivation[1].
Fmoc-FFC (up to 128 μg/mL; 24 h) maintained >80% viability in HEK-293T and HUVEC cells[1].
Fmoc-FFC (up to 256 μg/mL; 1 h) caused <5% hemolysis of rabbit erythrocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK-293T and HUVEC human cell lines
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Concentration:up to 128 μg/mL (Fmoc-FFC, serial dilutions)
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Incubation Time:24 h
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Result:Cell viability remained above 80% even at concentrations up to 128 μg/mL.
In Vivo
Fmoc-FFC (10 mg/kg; i.p.; every 12 h; four doses) plus meropenem (10 mg/kg) achieves 100% survival and reduces organ bacterial burden in a lethal murine systemic infection model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (male, 6-8 weeks, 16-18 g)[1]
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Dosage:10 mg/kg
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Administration:i.p.; every 12 h; four doses
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Result:Fmoc-FFC monotherapy (10 mg/kg) group: all mice succumbed to infection within 48 h (0% survival).
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Animal Model:BALB/c (male, 6-8 weeks, 16-18 g)[1]
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Dosage:10 mg/kg plus meropenem 10 mg/kg
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Administration:i.p.; every 12 h; four doses
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Result:Fmoc-FFC (10 mg/kg) plus meropenem (10 mg/kg) resulted in 100% survival over 96 h.
The combination produced a multi-log reduction in bacterial counts in the liver, spleen, and kidneys compared with meropenem alone.
Chemical Information
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Peso molecular 621.75
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Fòrmula C36H35N3O5S
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SMILES
O=C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CS)C(O)=O)=O)[C@@H](NC(CC2C3=C(C=CC=C3)C4=C2C=CC=C4)=O)CC5=CC=CC=C5
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fmoc-FFC Related Classifications
HY-189634 Related Classifications
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