Hydroxy-Dynasore
Based on 18 publication(s) in Google Scholar
Hydroxy Dynasore (Dyngo-4a), a structural mimetic analog of Dynasore (HY-15304), is an improved, less cytotoxic and versatile dynamin inhibitor with IC50 values of 0.38 μM and 2.3 μM for brain recombinant dynamin I and recombinant mouse dynamin II, respectively. Hydroxy Dynasore inhibits dynamin-dependent transferrin endocytosis with an IC50 of 5.7 μM.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.11%
- No. CAS: 1256493-34-1
- Fòrmula: C18H14N2O5
- Peso molecular:338.31
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Hydroxy-Dynasore
More- Nature. 2025 May;641(8062):427-437. [Abstract]
- Immunity. 2025 Nov 11;58(11):2670-2684.e10. [Abstract]
- Nat Cell Biol. 2026 Apr;28(4):812-827. [Abstract]
- Sci Bull. 2026 Mar 2:S2095-9273(26)00213-6. [Abstract]
- Adv Funct Mater. 2024 Oct 31.
- Nat Commun. 2025 Dec 15. [Abstract]
- Adv Sci (Weinh). 2023 Nov;10(33):e2300245. [Abstract]
- J Immunother Cancer. 2026 Jan 12;14(1):e013317. [Abstract]
- Arterioscler Thromb Vasc Biol. 2024 Jun;44(6):1225-1245. [Abstract]
- Int J Pharm. 2025 Jun 20:125881. [Abstract]
- ACS Appl Nano Mater. 2025 Mar 19.
- Commun Biol. 2024 Dec 31;7(1):1719. [Abstract]
- Traffic. 2023 Jan;24(1):20-33. [Abstract]
- Cells Tissues Organs. 2025 Apr 16:1-29. [Abstract]
- Genes Cells. 2025 Nov;30(6):e70063. [Abstract]
- bioRxiv. 2026 Jun 19.
- University of Toronto. 2025.
- bioRxiv. 2025 May 02.
Customer Validation & Images
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Others
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IF
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IF
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Cell Imaging/Staining
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Cell Imaging/Staining
Actividad biológica
Dynamin is a large GTPase enzyme that severs membrane-bound clathrin-coated vesicles. Endocytosis internalizes portions of cells’ plasma membrane along with extracellular material, is fundamentally important in cell physiology. Hydroxy Dynasore inhibits Dynamin I (Dyn I) activity with IC50 values of 2.7 μM and 0.38 μM with or without 0.06% Tween-80 in the GTPase assay[1]. Hydroxy Dynasore shows an IC50 of 5.7 μM in clathrin-mediated endocytosis (CME) assay for inhibition of Tfn-A594 uptake in U2OS cells[1]. Hydroxy Dynasore shows IC50 values of 0.38 μM and 1.1 μM in absence of Tween-80, and exhibits IC50 values of 4.9 μM and 30.0 μM in presence of Tween-80, respectively. Hydroxy Dynasore is 2.1-fold selective for DynI versus DynII from Sf21 cells and DynII (recombinant protein from Sf21 cells) in this GTPase assay[1]. Hydroxy Dynasore prevents the uptake of BoNT/A-Hc in cultured hippocampal neurons and in motor nerve terminals[2]. Hydroxy Dynasore (1-100μM; 20 min prior to the addition of Alexa Fluor 488-BoNT/A-Hc) results in depolarization of Hippocampal neurons, it dose-dependently inhibits internalization of Alexa Fluor 488-BoNT/A-Hc with an IC50 of 16.0 ± 1.2 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CD-1 mice[2].
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Dosage:30 mg/kg
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Administration:Intraperitoneal injection; 1.5–2 h before BoNT/A injection
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Result:Protected BoNT/A-induced paralysis in vivo.
Chemical Information
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No. CAS 1256493-34-1
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Appearance Solid
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Peso molecular 338.31
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Fòrmula C18H14N2O5
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Color Light brown to brown
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SMILES
OC1=CC2=CC=CC=C2C=C1C(N/N=C/C3=C(O)C=C(O)C(O)=C3)=O
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Synonyms
Dyngo-4a
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (18)
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Journal Impact Factor
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Most Recent
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Nature
2025 May;641(8062):427-437. PMID: 40205038 -
Immunity
Membrane integrity changes upon viral infection activate sphingomyelinase SMPDL3B to restrict cGAS-STING signaling via cGAMP degradation. [Abstract]2025 Nov 11;58(11):2670-2684.e10. PMID: 41175872 -
Nat Cell Biol
TOLLIP targets GSDME-NT-carrying endocytic vesicles for autophagy to regulate pyroptosis and chemotherapy efficacy. [Abstract]2026 Apr;28(4):812-827. PMID: 41803502 -
Sci Bull
A germline-specific vesicular structure licenses mRNA activation during spermiogenesis and is hijacked in gastric cancer. [Abstract]2026 Mar 2:S2095-9273(26)00213-6. PMID: 41925442 -
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Nat Commun
2025 Dec 15. PMID: 41397976 -
Adv Sci (Weinh)
Early Endosomes Undergo Calcium-Triggered Exocytosis and Enable Repair of Diffuse and Focal Plasma Membrane Injury. [Abstract]2023 Nov;10(33):e2300245. PMID: 37705135
Hydroxy-Dynasore purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2300245. [Abstract]
Images of Calcein-labeled cells injured by SLO after treatment either with DMSO (-) or with 2nd generation dynamin inhibitor Hydroxy-Dynasore (30 μM, 24 h) (+).
Hydroxy-Dynasore purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Nov;10(33):e2300245. [Abstract]
Images showing cells subjected to initial injury followed by Calcein labeling prior to second injury (pre-injury) or following second injury (post-injury) in cells treated with (+) or without Hydroxy-Dynasore (30 μM, 24 h) (-).
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J Immunother Cancer
Fully human anti-B7-H4 antibody induces lysosome-dependent ferroptosis to reverse primary resistance to PD-1 blockade. [Abstract]2026 Jan 12;14(1):e013317. PMID: 41526167 -
Arterioscler Thromb Vasc Biol
Novel Angiogenesis Role of GLP-1(32-36) to Rescue Diabetic Ischemic Lower Limbs via GLP-1R-Dependent Glycolysis in Mice. [Abstract]2024 Jun;44(6):1225-1245. PMID: 38511325
Hydroxy-Dynasore purchased from MedChemExpress. Usage Cited in: Arterioscler Thromb Vasc Biol. 2024 Jun;44(6):1225-1245. [Abstract]
Mouse primary bone marrow endothelial progenitor cells (EPCs) isolated from wild-type and Glp1r-/- mice were incubated with 100 μM Cy5 (cyanine 5)-tagged GLP-1(32-36) with or without dynamin inhibitor Hydroxy-Dynasore (Dyngo-4A) (30 μM) for 30 minutes and then imaged with spinning disk confocal microscope.
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Int J Pharm
2025 Jun 20:125881. PMID: 40544975
Hydroxy-Dynasore purchased from MedChemExpress. Usage Cited in: Int J Pharm. 2025 Jun 20:125881. [Abstract]
Cellular colocalization of Fe3O4@PDA@Ru in U87-MG cells. Confocal fluorescence micrographs of U87-MG cells cocultured with Fe3O4@PDA@Ru at 37 °C (control) and 4 °C, as well as cells pretreated with Hydroxy-Dynasore (Dyngo-4a) (0.5 μM, 4 h), cytochalasin D, chlorpromazine, and nystatin, respectively.
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Hydroxy-Dynasore purchased from MedChemExpress. Usage Cited in: ACS Appl Nano Mater. 2025 Mar 19.
Cellular colocalization of Fe3O4@PDA@Ru in U87-MG cells. Confocal fluorescence micrographs of U87-MG cells cocultured with Fe3O4@PDA@Ru at 37 °C (control) and 4 °C, as well as cells pretreated with Hydroxy-Dynasore (Dyngo-4a) (0.5 μM, 4 h), cytochalasin D, chlorpromazine, and nystatin, respectively.
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Commun Biol
Proteolysis targeting chimera (PROTAC)-driven antibody internalization of oncogenic cell surface receptors. [Abstract]2024 Dec 31;7(1):1719. PMID: 39741170 -
Traffic
AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice. [Abstract]2023 Jan;24(1):20-33. PMID: 36412210 -
Cells Tissues Organs
Endocytotic Albumin Uptake Pathways in Human Adipose Stem Cells and Connection to Intracellular Calcium Oscillations and the Neonatal Fc receptor. [Abstract]2025 Apr 16:1-29. PMID: 40239638 -
Genes Cells
Cell Surface LAMP-2C Is Internalized in a Clathrin-Dependent Manner and Transported to the Recycling and Lysosomal Pathways. [Abstract]2025 Nov;30(6):e70063. PMID: 41196290 -
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Solvente y solubilidad
DMSO : 50 mg/mL (147.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Adam McCluskey, et al. Building a Better Dynasore: The Dyngo Compounds Potently Inhibit Dynamin and Endocytosis. Traffic. 2013 Dec;14(12):1272-89. [Content Brief]
[2]. Callista B Harper, et al.Dynamin Inhibition Blocks Botulinum Neurotoxin Type A Endocytosis in Neurons and Delays Botulism.J Biol Chem. 2011 Oct 14;286(41):35966-76. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9559 mL | 14.7793 mL | 29.5587 mL | 73.8967 mL |
| 5 mM | 0.5912 mL | 2.9559 mL | 5.9117 mL | 14.7793 mL | |
| 10 mM | 0.2956 mL | 1.4779 mL | 2.9559 mL | 7.3897 mL | |
| 15 mM | 0.1971 mL | 0.9853 mL | 1.9706 mL | 4.9264 mL | |
| 20 mM | 0.1478 mL | 0.7390 mL | 1.4779 mL | 3.6948 mL | |
| 25 mM | 0.1182 mL | 0.5912 mL | 1.1823 mL | 2.9559 mL | |
| 30 mM | 0.0985 mL | 0.4926 mL | 0.9853 mL | 2.4632 mL | |
| 40 mM | 0.0739 mL | 0.3695 mL | 0.7390 mL | 1.8474 mL | |
| 50 mM | 0.0591 mL | 0.2956 mL | 0.5912 mL | 1.4779 mL | |
| 60 mM | 0.0493 mL | 0.2463 mL | 0.4926 mL | 1.2316 mL | |
| 80 mM | 0.0369 mL | 0.1847 mL | 0.3695 mL | 0.9237 mL | |
| 100 mM | 0.0296 mL | 0.1478 mL | 0.2956 mL | 0.7390 mL |
Hydroxy-Dynasore Related Classifications
HY-13863 Related Classifications
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