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Referencia número: HY-183876

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LAF-153

LAF-153 is a Methionine Aminopeptidase-2 (MetAP-2) inhibitor with human IC₅₀ values of 0.074 μM and 0.16 μM.LAF-153 has a t-butyl group extending into the innermost portion of the MetAP-2 cleft, a lactam ring coordinated in the enzyme’s shallow surface pocket, and three hydroxyl groups coordinating to the two cobalt ions in the enzyme’s active site.

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No. CAS: 661482-39-9
Fòrmula: C₁₈H₃₂N₂O₇
Peso molecular:388.46

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

LAF-153 Chemical Structure

No. CAS: 661482-39-9

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- Cellular Effect
- In Vitro
Chemical Information
Protocolo (1)
Pureza y Documentación
Referencias
Calculators
Help & FAQs
Classifications
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Actividad biológica

Descripciòn

Ver todos los productos específicos de isoformas Aminopeptidase :

CD13
LTA4H

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	150 μM Compound: 9c	Cytotoxicity in human HeLa cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human HeLa cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
HepG2	IC₅₀	150 μM Compound: 9c	Cytotoxicity in human HepG2 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human HepG2 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
HL-60	IC₅₀	150 μM Compound: 9c	Cytotoxicity in human HL60 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human HL60 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
KB	IC₅₀	62.6 μM Compound: 9c	Cytotoxicity in human KB cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human KB cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
Lu1	IC₅₀	23.4 μM Compound: 9c	Cytotoxicity in human Lu1 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human Lu1 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]
MCF7	IC₅₀	23.4 μM Compound: 9c	Cytotoxicity in human MCF7 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry Cytotoxicity in human MCF7 cells assessed as reduction in cell growth incubated for 72 hrs by neutral red staining based colorimetry	[PMID: 30108762]

In Vitro

LAF-153 reversibly binds to human MetAP-2 via coordination of its hydroxyl groups to the enzyme's cobalt ions, with its t-butyl group and lactam ring occupying distinct pockets in the enzyme cleft^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

No. CAS 661482-39-9
Peso molecular 388.46
Fòrmula C₁₈H₃₂N₂O₇
SMILES

CC(C)(C)/C=C/[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC)C(N[C@@H]1C(NC[C@@H](CC1)O)=O)=O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocolo

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Pureza y Documentación

Instrucciones de manejo (2659 KB)

Referencias

[1]. Heinrich T, et al. Discovery and structure-based optimization of next-generation reversible methionine aminopeptidase-2 (MetAP-2) inhibitors[J]. Journal of medicinal chemistry, 2019, 62(10): 5025-5039. [Content Brief]

Calculators

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Molecular Weight Molecular Weight

Calculadora de dilución

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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