ML251
Based on 1 publication(s) in Google Scholar
ML251, a potent nanomolar T. brucei and T. cruzi phosphofructokinase (PFK) inhibitor, inhibits T. brucei PFK (IC50=0.37 μM) and T. cruzi PFK (IC50=0.13 μM). ML251 can be used for the research of parasite.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.62%
- No. CAS: 1486482-16-9
- Fòrmula: C17H13Cl2N3O4S
- Peso molecular:426.27
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ML251
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Actividad biológica
Descripciòn
IC50 & Target
IC50: 0.37 μM (T. brucei PFK)[1].
IC50: 0.13 μM (T. cruzi PFK)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MRC5 | ED50 |
46 μM
Compound: 30, ML251
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Cytotoxicity against human MRC5 cells assessed as intracellular ATP content after 72 hrs by Cell titer-Glo luminiscent assay
Cytotoxicity against human MRC5 cells assessed as intracellular ATP content after 72 hrs by Cell titer-Glo luminiscent assay
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[PMID: 24900769] |
In Vitro
ML251 (T. brucei; 0~33.3 μg/mL) produces modest dose-dependent toxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
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Cell Line:T. brucei
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Concentration:0~33.3 μg/mL
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Incubation Time:
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Result:Dose-dependent reduction of cell viability.
Chemical Information
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No. CAS 1486482-16-9
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Appearance Solid
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Peso molecular 426.27
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Fòrmula C17H13Cl2N3O4S
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Color White to off-white
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SMILES
O=[S](NC1=NOC=C1)(C(C=C2)=CC=C2NC(CC(C=C3)=CC(Cl)=C3Cl)=O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Commun
Tumor-derived GDF15 induces CCN3⁺ Schwann cells to promote cancer pain in pancreatic cancer. [Abstract]2026 May 12. PMID: 42120379
Solvente y solubilidad
In Vitro:
DMSO : 250 mg/mL (586.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (4.88 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3459 mL | 11.7297 mL | 23.4593 mL | 58.6483 mL |
| 5 mM | 0.4692 mL | 2.3459 mL | 4.6919 mL | 11.7297 mL | |
| 10 mM | 0.2346 mL | 1.1730 mL | 2.3459 mL | 5.8648 mL | |
| 15 mM | 0.1564 mL | 0.7820 mL | 1.5640 mL | 3.9099 mL | |
| 20 mM | 0.1173 mL | 0.5865 mL | 1.1730 mL | 2.9324 mL | |
| 25 mM | 0.0938 mL | 0.4692 mL | 0.9384 mL | 2.3459 mL | |
| 30 mM | 0.0782 mL | 0.3910 mL | 0.7820 mL | 1.9549 mL | |
| 40 mM | 0.0586 mL | 0.2932 mL | 0.5865 mL | 1.4662 mL | |
| 50 mM | 0.0469 mL | 0.2346 mL | 0.4692 mL | 1.1730 mL | |
| 60 mM | 0.0391 mL | 0.1955 mL | 0.3910 mL | 0.9775 mL | |
| 80 mM | 0.0293 mL | 0.1466 mL | 0.2932 mL | 0.7331 mL | |
| 100 mM | 0.0235 mL | 0.1173 mL | 0.2346 mL | 0.5865 mL |
ML251 Related Classifications
HY-12607 Related Classifications
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