PC190723
Based on 1 Customer Validation
PC190723 is a bacterial cell division protein FtsZ inhibitor (IC50 = 55 nM). PC 190723 prevents cell division. PC190723 has potent and selective bactericidal activity against staphylococci, including methicillin- and multi-drug-resistant Staphylococcus aureus. PC190723 induces nucleated assembly of Bs-FtsZ into single-stranded coiled protofilaments and polymorphic condensates. PC190723 can be studied in anti-bacterial infection research.
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- Pureza: 98.67%
- No. CAS: 951120-33-5
- Fòrmula: C14H8ClF2N3O2S
- Peso molecular:355.75
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
IC50: 55 ng/mL (FtsZ)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
>64 μg/mL
Compound: PC190723
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Cytotoxicity against human HepG2 cells
Cytotoxicity against human HepG2 cells
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[PMID: 18801997] |
| L929 | IC50 |
>100 μM
Compound: 1; PC190723
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Cytotoxicity against mouse L929 cells by MTS assay
Cytotoxicity against mouse L929 cells by MTS assay
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[PMID: 30503946] |
PC190723 causes a reduction in viable S. aureus cell numbers of more than three logarithms within 24 hours but is inactive against a range of other Gram-positive and Gram-negative pathogenic bacteria[1].
PC190723 (0.1 nM-100 μM) directly inhibits the GTPase activity of FtsZ in a concentration-dependent manner with an IC50 of 55 nM[1].
PC190723 (2 μM, 2 h) causes maximal enlargement of S. aureus cells within 2 hours of exposure[1].
PC190723 directly blocks FtsZ activity to prevent septum formation and cell division[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:S. aureus treated mice[1]
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Dosage:30 mg/kg
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Administration:Subcutaneous/intravenous injection, single dose
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Result:Resulted in a 100% survival of mice inoculated intraperitoneally with a potentially lethal dose of S. aureus, as compared with a 0% survival for the group receiving no compound.
Produced dose-dependent efficacy for both routes of administration with ED50 were 7.3 and 10.2 mg/kg for S.C. and I.V. administration, respectively.
Chemical Information
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No. CAS 951120-33-5
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Appearance Solid
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Peso molecular 355.75
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Fòrmula C14H8ClF2N3O2S
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Color Light yellow to yellow
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SMILES
NC(C1=C(C(OCC2=NC3=C(S2)N=CC(Cl)=C3)=CC=C1F)F)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 200 mg/mL (562.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (14.05 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (14.05 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Haydon DJ, et al. An inhibitor of FtsZ with potent and selective anti-staphylococcal activity. Science. 2008 Sep 19;321(5896):1673-5. [Content Brief]
[2]. Buroni S, et al. The cell division protein FtsZ as a cellular target to hit cystic fibrosis pathogens. Eur J Med Chem. 2020 Mar 15;190:112132. [Content Brief]
[3]. Andreu JM, et al. The antibacterial cell division inhibitor PC190723 is an FtsZ polymer-stabilizing agent that induces filament assembly and condensation. J Biol Chem. 2010 May 7;285(19):14239-46. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8110 mL | 14.0548 mL | 28.1096 mL | 70.2741 mL |
| 5 mM | 0.5622 mL | 2.8110 mL | 5.6219 mL | 14.0548 mL | |
| 10 mM | 0.2811 mL | 1.4055 mL | 2.8110 mL | 7.0274 mL | |
| 15 mM | 0.1874 mL | 0.9370 mL | 1.8740 mL | 4.6849 mL | |
| 20 mM | 0.1405 mL | 0.7027 mL | 1.4055 mL | 3.5137 mL | |
| 25 mM | 0.1124 mL | 0.5622 mL | 1.1244 mL | 2.8110 mL | |
| 30 mM | 0.0937 mL | 0.4685 mL | 0.9370 mL | 2.3425 mL | |
| 40 mM | 0.0703 mL | 0.3514 mL | 0.7027 mL | 1.7569 mL | |
| 50 mM | 0.0562 mL | 0.2811 mL | 0.5622 mL | 1.4055 mL | |
| 60 mM | 0.0468 mL | 0.2342 mL | 0.4685 mL | 1.1712 mL | |
| 80 mM | 0.0351 mL | 0.1757 mL | 0.3514 mL | 0.8784 mL | |
| 100 mM | 0.0281 mL | 0.1405 mL | 0.2811 mL | 0.7027 mL |
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