Picoxystrobin
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Picoxystrobin is a strobilurin fungicide. Picoxystrobin controls plant diseases by inhibiting mitochondrial respiration. Picoxystrobin is highly toxic to zebrafish embryos, causing developmental abnormalities, oxidative stress, and immunotoxicity.
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- Pureza: 99.76%
- No. CAS: 117428-22-5
- Fòrmula: C18H16F3NO4
- Peso molecular:367.32
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Picoxystrobin (60-105 µg/L; waterborne exposure; 96 h) causes developmental toxicity, oxidative stress and immunotoxicity in zebrafish embryos[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wild type AB-strain zebrafish embryos[2]
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Dosage:60, 69, 79, 91, 105 µg/L
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Administration:Embryos at 2 hpf were exposed to picoxystrobin solutions in 24-well culture plates (1 embryo per well), and the exposure solution was renewed every 24 h for 96 h
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Result:Showed high toxicity to zebrafish embryos with a 96-h LC50 of 86 µg/L, causing decreased heartbeat, hatching inhibition, growth regression, morphological deformities, increased ROS and MDA contents, decreased SOD activity and GSH content, induced CAT activity, and altered expressions of genes related to antioxidant system and innate immune system.
Chemical Information
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No. CAS 117428-22-5
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Appearance Solid
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Peso molecular 367.32
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Fòrmula C18H16F3NO4
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Color Light yellow to brown
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SMILES
O=C(OC)/C(C(C=CC=C1)=C1COC2=CC=CC(C(F)(F)F)=N2)=C/OC
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (272.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Korean - KR (597 KB)
- Portuguese - PT (597 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Wei Jia, et al. Effects of two strobilurins (azoxystrobin and picoxystrobin) on embryonic development and enzyme activities in juveniles and adult fish livers of zebrafish (Danio rerio). Chemosphere. 2018 Sep;207:573-580. [Content Brief]
[2]. Li H, et al. Developmental toxicity, oxidative stress and immunotoxicity induced by three strobilurins (pyraclostrobin, trifloxystrobin and picoxystrobin) in zebrafish embryos. Chemosphere. 2018 Sep;207:781-790. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7224 mL | 13.6121 mL | 27.2242 mL | 68.0605 mL |
| 5 mM | 0.5445 mL | 2.7224 mL | 5.4448 mL | 13.6121 mL | |
| 10 mM | 0.2722 mL | 1.3612 mL | 2.7224 mL | 6.8061 mL | |
| 15 mM | 0.1815 mL | 0.9075 mL | 1.8149 mL | 4.5374 mL | |
| 20 mM | 0.1361 mL | 0.6806 mL | 1.3612 mL | 3.4030 mL | |
| 25 mM | 0.1089 mL | 0.5445 mL | 1.0890 mL | 2.7224 mL | |
| 30 mM | 0.0907 mL | 0.4537 mL | 0.9075 mL | 2.2687 mL | |
| 40 mM | 0.0681 mL | 0.3403 mL | 0.6806 mL | 1.7015 mL | |
| 50 mM | 0.0544 mL | 0.2722 mL | 0.5445 mL | 1.3612 mL | |
| 60 mM | 0.0454 mL | 0.2269 mL | 0.4537 mL | 1.1343 mL | |
| 80 mM | 0.0340 mL | 0.1702 mL | 0.3403 mL | 0.8508 mL | |
| 100 mM | 0.0272 mL | 0.1361 mL | 0.2722 mL | 0.6806 mL |
Picoxystrobin Related Classifications
HY-136355 Related Classifications
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