Prostate Specific Antigen Substrate
Based on 1 Customer Validation
Prostate Specific Antigen Substrate is a highly specific fluorescent peptide substrate can be used for measuring prostate-specific antigen (PSA) enzymatic activity.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.79%
- No. CAS: 380225-52-5
- Fòrmula: C44H64N12O13
- Peso molecular:969.05
-
Almacenamiento:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Prostate Specific Antigen Substrate (400 μM; 120-minute) is robustly hydrolyzed by enzymatically active PSA purified from human seminal plasma, but not by immunoprecipitated PSA from transfected AT2, AT6, PC3, and DU145 prostate cancer cell lines[1].
Prostate Specific Antigen Substrate can screen for proteolytic activity of immunoprecipitated PSA samples[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
No. CAS 380225-52-5
-
Appearance Solid
-
Peso molecular 969.05
-
Fòrmula C44H64N12O13
-
Color White to off-white
-
Sequence
{Mu}-His-Ser-Ser-Lys-Leu-Gln-{AMC}
-
Sequence Shortening
{Mu}-HSSKLQ-{AMC}
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
DMSO : 100 mg/mL (103.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (272 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0319 mL | 5.1597 mL | 10.3194 mL | 25.7985 mL |
| 5 mM | 0.2064 mL | 1.0319 mL | 2.0639 mL | 5.1597 mL | |
| 10 mM | 0.1032 mL | 0.5160 mL | 1.0319 mL | 2.5798 mL | |
| 15 mM | 0.0688 mL | 0.3440 mL | 0.6880 mL | 1.7199 mL | |
| 20 mM | 0.0516 mL | 0.2580 mL | 0.5160 mL | 1.2899 mL | |
| 25 mM | 0.0413 mL | 0.2064 mL | 0.4128 mL | 1.0319 mL | |
| 30 mM | 0.0344 mL | 0.1720 mL | 0.3440 mL | 0.8599 mL | |
| 40 mM | 0.0258 mL | 0.1290 mL | 0.2580 mL | 0.6450 mL | |
| 50 mM | 0.0206 mL | 0.1032 mL | 0.2064 mL | 0.5160 mL | |
| 60 mM | 0.0172 mL | 0.0860 mL | 0.1720 mL | 0.4300 mL | |
| 80 mM | 0.0129 mL | 0.0645 mL | 0.1290 mL | 0.3225 mL | |
| 100 mM | 0.0103 mL | 0.0516 mL | 0.1032 mL | 0.2580 mL |
Powered by Bioz