RH01386
Based on 1 Customer Validation
RH01386 is a small molecule that can prevent endoplasmic reticulum stress (ERS)-induced β cell dysfunction and death, and inhibits proapoptotic gene expression. RH01386 restores ERS-impaired glucose-stimulated insulin secretion responses. RH01386 has the potential for type 2 diabetes treatment.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.66%
- No. CAS: 301177-36-6
- Fòrmula: C18H15F3N4O3S
- Peso molecular:424.40
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
ER stress-induced β cell dysfunction[1]
In Vitro
RH01386 (270 nM-70 μM; 72 hours) is confirmed to increase ATP levels in βTC6 cells treated with (tunicamycin) Tm, exhibits a EC50 value of 1.894 μM[1]. RH01386 (270 nM-70 μM; 72 hours) dose-dependently inhibits (tunicamycin) Tm-induced cell death, and inhibits the activity of caspase-3, a downstream effector of the apoptotic pathway in βTC6 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:βTC6 cells
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Concentration:270 nM-70 μM
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Incubation Time:72 hours
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Result:Inhibited ER stress-induced death of βTC6 cells in a dose-dependent manner.
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Cell Line:βTC6 cells
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Concentration:270 nM-70 μM
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Incubation Time:72 hours
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Result:Protected βTC6 cells from Tm-induced cell death.
Chemical Information
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No. CAS 301177-36-6
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Appearance Solid
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Peso molecular 424.40
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Fòrmula C18H15F3N4O3S
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Color Off-white to light yellow
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SMILES
N#CC1=C(C2CCCCC2)N=C(SC3=CC=C(C(F)(F)F)C=C3[N+]([O-])=O)NC1=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (235.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.89 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3563 mL | 11.7813 mL | 23.5627 mL | 58.9067 mL |
| 5 mM | 0.4713 mL | 2.3563 mL | 4.7125 mL | 11.7813 mL | |
| 10 mM | 0.2356 mL | 1.1781 mL | 2.3563 mL | 5.8907 mL | |
| 15 mM | 0.1571 mL | 0.7854 mL | 1.5708 mL | 3.9271 mL | |
| 20 mM | 0.1178 mL | 0.5891 mL | 1.1781 mL | 2.9453 mL | |
| 25 mM | 0.0943 mL | 0.4713 mL | 0.9425 mL | 2.3563 mL | |
| 30 mM | 0.0785 mL | 0.3927 mL | 0.7854 mL | 1.9636 mL | |
| 40 mM | 0.0589 mL | 0.2945 mL | 0.5891 mL | 1.4727 mL | |
| 50 mM | 0.0471 mL | 0.2356 mL | 0.4713 mL | 1.1781 mL | |
| 60 mM | 0.0393 mL | 0.1964 mL | 0.3927 mL | 0.9818 mL | |
| 80 mM | 0.0295 mL | 0.1473 mL | 0.2945 mL | 0.7363 mL | |
| 100 mM | 0.0236 mL | 0.1178 mL | 0.2356 mL | 0.5891 mL |
RH01386 Related Classifications
HY-124771 Related Classifications
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