DCAF1 - DDB1 and CUL4 associated factor 1 Gene

Also Known as RIP; VPRBP

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 9730

About DCAF1

Cytogenetic location: 3p21.2 Genomic coordinates (GRCh38): 3:51,395,867-51,505,639 (from NCBI)

This gene has 4 transcripts (splice variants) and 254 orthologues. Broad expression in testis (RPKM 27.5), thyroid (RPKM 8.3) and 24 other tissues.

Summary

Enables Estrogen receptor binding activity and histone kinase activity (H2A-T120 specific). Involved in cell competition in a multicellular organism; histone H2A-T120 phosphorylation; and negative regulation of transcription by RNA polymerase II. Located in fibrillar center and nucleoplasm. Part of Cul4-RING E3 ubiquitin Ligase complex. Colocalizes with COP9 signalosome. [provided by Alliance of Genome Resources, Apr 2022]

DCAF1 Products (12)

mRNA	Protein	Name
NM_001171904.2	NP_001165375.1	DDB1- and CUL4-associated factor 1 isoform 2
NM_001349168.2	NP_001336097.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001349169.2	NP_001336098.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001349170.2	NP_001336099.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001349171.2	NP_001336100.1	DDB1- and CUL4-associated factor 1 isoform 3
NM_001387578.1	NP_001374507.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001387579.1	NP_001374508.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001387580.1	NP_001374509.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001387581.1	NP_001374510.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001387582.1	NP_001374511.1	DDB1- and CUL4-associated factor 1 isoform 2
NM_001387583.1	NP_001374512.1	DDB1- and CUL4-associated factor 1 isoform 3
NM_014703.3	NP_055518.1	DDB1- and CUL4-associated factor 1 isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables histone H2AT120 kinase activity	IDA IDA: Inferred from direct assay	24140421	GOA
enables nuclear estrogen receptor binding	IPI IPI: Inferred from physical interaction	28068668	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	16949367	GOA
enables ubiquitin-like ligase-substrate adaptor activity	IDA IDA: Inferred from direct assay	23063525	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in cell competition in a multicellular organism	IMP IMP: Inferred from mutant phenotype	20644714	GOA
involved in negative regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	24140421	GOA
involved in positive regulation of protein catabolic process	IDA IDA: Inferred from direct assay	23063525	GOA
involved in post-translational protein modification	IDA IDA: Inferred from direct assay	24140421	GOA
involved in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	28242748	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of COP9 signalosome	IDA IDA: Inferred from direct assay	18850735	GOA
part of Cul4-RING E3 ubiquitin ligase complex	IDA IDA: Inferred from direct assay	16949367	GOA
is active in centrosome	IDA IDA: Inferred from direct assay	28242748	GOA
located in nucleus	IDA IDA: Inferred from direct assay	20178741	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Protein Preferred Names	Protein Names
DDB1- and CUL4-associated factor 1	HIV-1 Vpr-binding protein

DCAF1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	DCAF1	Q9Y4B6	DDB1	Homo sapiens	Q16531	Density Sedimentation	24412650
Intra	DCAF1	Q9Y4B6	LATS2	Homo sapiens	Q9NRM7	Anti Tag CoIP	25026211
Intra	DCAF1	Q9Y4B6	LATS2	Homo sapiens	Q9NRM7	Ubiquitinase Assay	25026211
Intra	DCAF1	Q9Y4B6	LATS1	Homo sapiens	O95835	Anti Tag CoIP	25026211
Intra	DCAF1	Q9Y4B6	LATS1	Homo sapiens	O95835	Pull Down	25026211
Intra	DCAF1	Q9Y4B6	LATS1	Homo sapiens	O95835	Ubiquitinase Assay	25026211
Cross	DCAF1	Q9Y4B6	a0a0h3lac5_mycte	Mycobacterium tuberculosis	A0A0H3LAC5	Anti Bait CoIP	30118682
Cross	DCAF1	Q9Y4B6	vpr	Human immunodeficiency virus	P12520	Pull Down	22190034
Cross	DCAF1	Q9Y4B6	vpx	Human immunodeficiency virus	P18045	Anti Tag CoIP	19264781
Cross	DCAF1	Q9Y4B6	vpx	Simian immunodeficiency virus	P19508	GMS	24336198
Cross	DCAF1	Q9Y4B6	vpx	Simian immunodeficiency virus	P19508	X-Ray Diffraction	24336198
Cross	DCAF1	Q9Y4B6	vpx	Simian immunodeficiency virus	P19508	Anti Tag CoIP	19264781

Cross: Cross-species interaction Intra: Intraspecies interaction

DCAF1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P89566	VPRBP Antibody (YA8910)	WB, ICC/IF, IF-Tissue, IP, ELISA	human, mouse, rat

Related Diseases

Diseases	Alias
Intraorbital Meningioma
Neurilemmomatosis	Schwannomatosis Neurofibromatosis Type 3 Nf3 Neurilemmomatosis Congenital Cutaneous Neurinomatosis Congenital Cutaneous Neurilemmomatosis Multiple Neurilemmomas Multiple Schwannomas Neurilemmomatosis, Congenital Cutaneous Schwannomatosis 1 Neurofibromatosis 3 Mixed Central And Peripheral Neurofibromatosis Nf3 - [Neurofibromatosis Type 3]
Meningioma, Familial	Meningioma Familial Meningioma Meningioma, Familial, Susceptibility To Meningeal Neoplasm Meningeal Neoplasms Meningiomas Meningioma, Nf2-Related, Somatic Meningioma, Sis-Related Meningothelial Cell Tumor Neoplasm Of The Meninges Primary Meningeal Tumor Familial Multiple Meningioma MNGMA Meningioma, Benign, No Icd-O Subtype Intracranial Meningioma Meningothelial Cell Neoplasm Supratentorial Meningioma Primary Neoplasm Of Spinal Meninges Benign Intracranial Meningioma Benign Meningioma Meningeal Tumours Meningeal Sarcoma Of Unspecified Site Meningothelial Sarcoma Of Unspecified Site
Acoustic Neuroma	Neurofibromatosis Type 2 Vestibular Schwannoma Acoustic Neurinoma Bilateral Acoustic Neurofibromatosis Nf2 Acoustic Neurilemoma Cerebellopontine Angle Tumor Neurofibromatosis Central Type Neurofibromatosis Type Ii Vestibular Neurilemmoma Acoustic Tumor Neurinoma Of The Acoustic Nerve Acoustic Neurinoma Bilateral Acoustic Schwannomas Bilateral Banf Central Neurofibromatosis Familial Acoustic Neuromas Neurofibromatosis 2 Neurofibromatosis Type 2 Merlin Schwannoma, Acoustic, Bilateral Neuroma Acoustic Neuroma, Acoustic Familial Acoustic Neuroma Familial Vestibular Schwannoma Neurofibromatosis, Central Type Nf2 - [Neurofibromatosis Type 2]
Neurofibromatosis, Type Ii	Neurofibromatosis 2 Neurofibromatosis, Type 2 NF2 Neurofibromatosis Type Ii Bilateral Acoustic Neurofibromatosis Banf Acn Central Neurofibromatosis Neurofibromatosis, Central Type Acoustic Schwannomas, Bilateral Acoustic Neurinoma, Bilateral Bilateral Acoustic Neurinoma Bilateral Acoustic Schwannomas Familial Acoustic Neuromas
Cockayne Syndrome A	Cockayne Syndrome Type 1 Cockayne Syndrome, Type A Cockayne Syndrome Type I CSA Cockayne Syndrome Classic Form Cockayne Syndrome Classical Cockayne Syndrome Type A Ckn1
Aicardi-Goutieres Syndrome	Aicardi Goutieres Syndrome Cree Encephalitis Aicardi-Goutières Syndrome Encephalopathy With Basal Ganglia Calcification Ags Encephalopathy With Intracranial Calcification And Chronic Lymphocytosis Of Cerebrospinal Fluid Pseudotoxoplasmosis Syndrome Encephalopathy, Familial Infantile, With Calcification Of Basal Ganglia And Chronic Cerebrospinal Fluid Lymphocytosis Familial Infantile Encephalopathy With Intracranial Calcification And Chronic Cerebrospinal Fluid Lymphocytosis Aicardi-Goutieres Syndrome 1
Neurilemmoma	Schwannoma Benign Schwannoma Neurilemoma Peripheral Fibroblastoma Psammomatous Schwannoma Neurolemmoma Schwannomas
Lissencephaly	Pachygyria Broad Gyri Of Cerebrum Large Gyri Of Cerebrum Macrogyria

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (5)

Cat. No.	Product Name	Purity	Rare Diseases
HY-158301	MY-1B	99.94%	Unkown
HY-78695	JQ-1 carboxylic acid	99.69%	Unkown
HY-169152	DCAF1 ligand 1	/	Unkown
HY-169147	MY-11B	/	Unkown
HY-182974	DCAF1 binder 3	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Macaca mulatta	DCAF1	VGNC	VGNC:71819
Canis familiaris	DCAF1	VGNC	VGNC:39788
Felis catus	DCAF1	VGNC	VGNC:61353
Mus musculus	DCAF1	MGD	MGI:2445220
Bos taurus	DCAF1	VGNC	VGNC:27899
Rattus norvegicus	DCAF1	RGD	RGD:1305176
Others	DCAF1	NCBI