1. MAPK/ERK Pathway Stem Cell/Wnt PI3K/Akt/mTOR
  2. ERK Akt
  3. H-Ile-Lys-Val-Ala-Val-OH

H-Ile-Lys-Val-Ala-Val-OH is one of the most potent active sites of laminin-1. H-Ile-Lys-Val-Ala-Val-OH promotes cell adhesion, neurite outgrowth, and tumor growth. H-Ile-Lys-Val-Ala-Val-OH stimulates BMMSC population growth and proliferation by activating MAPK/ERK1/2 and PI3K/Akt signalling pathways.

For research use only. We do not sell to patients.

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H-Ile-Lys-Val-Ala-Val-OH Chemical Structure

H-Ile-Lys-Val-Ala-Val-OH Chemical Structure

CAS No. : 131167-89-0

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Based on 1 publication(s) in Google Scholar

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Description

H-Ile-Lys-Val-Ala-Val-OH is one of the most potent active sites of laminin-1. H-Ile-Lys-Val-Ala-Val-OH promotes cell adhesion, neurite outgrowth, and tumor growth. H-Ile-Lys-Val-Ala-Val-OH stimulates BMMSC population growth and proliferation by activating MAPK/ERK1/2 and PI3K/Akt signalling pathways[1][2].

IC50 & Target

ERK1

 

ERK2

 

Akt

 

In Vitro

H-Ile-Lys-Val-Ala-Val-OH (0-2.5 mM, 0-48 h) induces proliferation and proliferating cell nuclear antigen (PCNA) synthesis of bone marrow mesenchymal stem cell (BMMSC)[2].
H-Ile-Lys-Val-Ala-Val-OH (5 mM, 24 h) induces the BMMSC cell cycle for cells to enter S phase from G0/G1 and arrests them from entering G2/M phase[2].
H-Ile-Lys-Val-Ala-Val-OH (0-2.5 mM, 0-48 h) activats MAPK/ERK and PI3K/Akt signalling pathways by enhancing phosphorylation levels of ERK1/2 and Akt in the BMMSCs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: BMMSC
Concentration: 0, 0.004, 0.02, 0.1, 0.5 and 2.5 mM
Incubation Time: 0, 12, 24, 36, 48 h
Result: Stimulated proliferating cell nuclear antigen (PCNA) synthesis and induced proliferation of bone marrow mesenchymal stem cell (BMMSC) in a dose- and time-dependent manner.

Cell Cycle Analysis[2]

Cell Line: BMMSC
Concentration: 5 mM
Incubation Time: 24 h
Result: Induced the BMMSC cell cycle for cells to enter S phase from G0/G1 and arrested them from entering G2/M phase; the increased proportion of S phase in cell cycle was considered to be a sign of BMMSC proliferation.

Western Blot Analysis[2]

Cell Line: BMMSC
Concentration: 0, 0.004, 0.02, 0.1, 0.5 and 2.5 mM
Incubation Time: 0, 12, 24, 36, 48 h
Result: Increased significantly the levels of p-ERK1/2 and p-Akt in a dose- and time-dependent manner.
Molecular Weight

528.69

Formula

C25H48N6O6

CAS No.
Appearance

Solid

Color

White to off-white

Sequence

H-Ile-Lys-Val-Ala-Val-OH

Sequence Shortening

IKVAV

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (189.15 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8915 mL 9.4573 mL 18.9147 mL
5 mM 0.3783 mL 1.8915 mL 3.7829 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.8915 mL 9.4573 mL 18.9147 mL 47.2867 mL
5 mM 0.3783 mL 1.8915 mL 3.7829 mL 9.4573 mL
10 mM 0.1891 mL 0.9457 mL 1.8915 mL 4.7287 mL
15 mM 0.1261 mL 0.6305 mL 1.2610 mL 3.1524 mL
20 mM 0.0946 mL 0.4729 mL 0.9457 mL 2.3643 mL
25 mM 0.0757 mL 0.3783 mL 0.7566 mL 1.8915 mL
30 mM 0.0630 mL 0.3152 mL 0.6305 mL 1.5762 mL
40 mM 0.0473 mL 0.2364 mL 0.4729 mL 1.1822 mL
50 mM 0.0378 mL 0.1891 mL 0.3783 mL 0.9457 mL
60 mM 0.0315 mL 0.1576 mL 0.3152 mL 0.7881 mL
80 mM 0.0236 mL 0.1182 mL 0.2364 mL 0.5911 mL
100 mM 0.0189 mL 0.0946 mL 0.1891 mL 0.4729 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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H-Ile-Lys-Val-Ala-Val-OH
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