4-Methylbenzylidene camphor
Based on 2 publication(s) in Google Scholar
4-Methylbenzylidene camphor (4-MBC) is an endocrine disrupter that produces estrogen-like effects. 4-Methylbenzylidene camphor decreases the proliferation of human trophoblast cells and induces apoptosis. 4-Methylbenzylidene camphor activates PI3K/AKT and ERK1/2 signaling pathways and elevates intracellular ROS production. 4-Methylbenzylidene camphor is a ultraviolet (UV) filter and may hamper normal placental formation during early pregnancy.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 36861-47-9
- Formula: C18H22O
- Molecular Weight:254.37
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) 4-Methylbenzylidene camphor
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IHC
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Cell Proliferation/Viability Assay
Biological Activity
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PI3K |
Akt |
ERK1 |
ERK2 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | EC50 |
31.6 μM
Compound: Enzacamene
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Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis
Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis
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[PMID: 20966043] |
| HepG2 | EC50 |
31.6 μM
Compound: Enzacamene
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Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis
Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis
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[PMID: 20966043] |
4-Methylbenzylidene camphor (4-MBC; 5-400 μM; for 48 h) inhibits proliferation of HTR8/SVneo cell[1].
4-Methylbenzylidene camphor (10-50 μM; for 48 h) induces apoptotic cell death of human trophoblast cells[1].
4-Methylbenzylidene camphor (5-50 μM; for 48 h) increased the proportion of cells in the SubG1 phase[1].
4-Methylbenzylidene camphor (50 μM; for 48 h) reduces invasion of human trophoblast cells[1].
4-Methylbenzylidene camphor (50 μM; 5-120 min) activates PI3K/AKT and ERK1/2 signaling pathways in human trophoblast cells[1].
4-Methylbenzylidene camphor (20-50 μM; 24 h) significantly increases the expression of SEMA6 A, GPR56, ITGB4, EPHB4, NRP1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HTR8/SVneo cells
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Concentration:0, 5, 10, 20, 50, 100, 200, and 400 μM
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Incubation Time:48 h
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Result:Dose-dependently inhibited cell proliferation of HTR8/SVneo cell.
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Cell Line:HTR8/SVneo cells
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Concentration:10, 20, 50 μM
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Incubation Time:48 h
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Result:Early and late apoptotic cells was significantly increased at 20 μM and 50 μM.
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Cell Line:HTR8/SVneo cells
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Concentration:5, 10, 20, 50 μM
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Incubation Time:48 h
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Result:Gradually increased the proportion of cells in the SubG1 phase.
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Cell Line:HTR8/SVneo cells
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Concentration:50 μM
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Incubation Time:48 h
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Result:Revealed a significant reduction of 81.5% in invasiveness
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Cell Line:HTR8/SVneo cells
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Concentration:50 μM
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Incubation Time:0, 5, 15, 30, 60, 120 min
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Result:The phosphorylation of AKT and its downstream kinase protein, P70S6K, peaked at 5 and 15 min, respectively, subsequently decreased after 30 min, and then reactivated at 120 min
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Cell Line:HTR8/SVneo cells
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Concentration:20, 50 μM
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Incubation Time:24 h
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Result:Significantly increased the expression of semaphorin 6 A (SEMA6 A), GPR56, integrin subunit beta 4 (ITGB4), EPHB4, neuropilin 1 (NRP1).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 36861-47-9
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Appearance Solid
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Molecular Weight 254.37
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Formula C18H22O
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Color White to off-white
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SMILES
CC1(C)[C@@H]2CC[C@]1(C)C(/C2=C\C3=CC=C(C)C=C3)=O
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Synonyms
4-MBC; Enzacamene
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Aquat Toxicol
Reproductive toxicity and parental transmission effects of 4-methylbenzylidene camphor (4-MBC) exposure in adult zebrafish. [Abstract]2025 Jun:283:107334. PMID: 40157257 -
Reprod Toxicol
4-Methylbenzylidene-camphor inhibits proliferation and induces reactive oxygen species-mediated apoptosis of human trophoblast cells. [Abstract]2019 Mar:84:49-58. PMID: 30597193
4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar:84:49-58. [Abstract]
PCNA protein is detected (green) and nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue) in HTR8/SVneo cells treated with 50 μM 4-MBC for 24 h.
4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar:84:49-58. [Abstract]
4-Methylbenzylidene-camphor (4-MBC) time-dependently affects phosphorylation of protein kinases in HTR8/SVneo cells. Time-dependent phosphorylation of AKT, P70S6K, S6, GSK3β, ERK1/2, and P90RSK in response to 50 μM 4-MBC is estimated. Immunoblots are imaged to calculate normalized values by estimating the abundance of phosphorylated proteins relative to total proteins.
4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar:84:49-58. [Abstract]
4-Methylbenzylidene-camphor (4-MBC) dose-dependently affects phosphorylation of protein kinases in HTR8/SVneo cells. Concentration-dependent phosphorylation of AKT, P70S6K, S6, GSK3β, ERK1/2, and P90RSK in response to 4-MBC for 2 h is estimated. Immunoblots are imaged to calculate normalized values by estimating the abundance of phosphorylated proteins relative to total proteins.
4-Methylbenzylidene camphor purchased from MedChemExpress. Usage Cited in: Reprod Toxicol. 2019 Mar:84:49-58. [Abstract]
The effects of 4-MBC (50 μM) alone or with selective kinase inhibitors on proliferation of HTR8/SVneo cells. LY294002 (AKT inhibitor, 20 μM) and U0126 (ERK1/2 inhibitor, 20 μM) are co-treated with 4-MBC for 48 h in triplicate.
Solvent & Solubility
DMSO : 100 mg/mL (393.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.83 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.83 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Handling Instructions (2659 KB)
References
[1]. Changwon Yang, et al. 4-Methylbenzylidene-camphor inhibits proliferation and induces reactive oxygen species-mediated apoptosis of human trophoblast cells. Reprod Toxicol. 2019 Mar:84:49-58. [Content Brief]
[2]. Margret Schlumpf, et al. Developmental toxicity of UV filters and environmental exposure: a review. Int J Androl. 2008 Apr;31(2):144-51. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9313 mL | 19.6564 mL | 39.3128 mL | 98.2820 mL |
| 5 mM | 0.7863 mL | 3.9313 mL | 7.8626 mL | 19.6564 mL | |
| 10 mM | 0.3931 mL | 1.9656 mL | 3.9313 mL | 9.8282 mL | |
| 15 mM | 0.2621 mL | 1.3104 mL | 2.6209 mL | 6.5521 mL | |
| 20 mM | 0.1966 mL | 0.9828 mL | 1.9656 mL | 4.9141 mL | |
| 25 mM | 0.1573 mL | 0.7863 mL | 1.5725 mL | 3.9313 mL | |
| 30 mM | 0.1310 mL | 0.6552 mL | 1.3104 mL | 3.2761 mL | |
| 40 mM | 0.0983 mL | 0.4914 mL | 0.9828 mL | 2.4571 mL | |
| 50 mM | 0.0786 mL | 0.3931 mL | 0.7863 mL | 1.9656 mL | |
| 60 mM | 0.0655 mL | 0.3276 mL | 0.6552 mL | 1.6380 mL | |
| 80 mM | 0.0491 mL | 0.2457 mL | 0.4914 mL | 1.2285 mL | |
| 100 mM | 0.0393 mL | 0.1966 mL | 0.3931 mL | 0.9828 mL |
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