β-Neo-Endorphin
Based on 1 Customer Validation
β-Neo-Endorphin is an endogenous opioid peptide. β-Neo-Endorphin is a hypothalamic “big” Leu-enkephalin of porcine origin. β-Neo-Endorphin shows activation of the Erk1/2, MMP-2 and MMP-9.
For research use only. We do not sell to patients.
- Purity: 98.97%
- CAS No.: 77739-21-0
- Formula: C54H77N13O12
- Molecular Weight:1100.27
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
|
ERK1 |
ERK2 |
MMP-2 |
MMP-9 |
β-Neo-Endorphin (0-20 μM, 20 h) stimulates wound healing in human keratinocytes[2].
β-Neo-Endorphin (promotes wound healing through activation of the Erk1/2 signaling pathway in human keratinocytes[2].
β-Neo-Endorphin (0-20 μM, 12 h) stimulates keratinocyte migration through activation of metalloproteinase (MMP)-2 and -9[2].
β-Neo-Endorphin (12 h) stimulates migration in the fibroblast[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human keratinocytes (HaCaT)
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Concentration:0, 5, 10 and 20 μM
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Incubation Time:20 h
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Result:Significantly increased the wound closure. Had no observable effect on cell viability.
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Cell Line:Human keratinocytes (HaCaT)
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Concentration:0, 5, 10 and 20 μM
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Incubation Time:12 h
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Result:Activated Erk1/2, phosphorylated P90RSK and Elk-1, showed significant upregulation of MMP-2 and -9 expression.
Chemical Information
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CAS No. 77739-21-0
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Appearance Solid
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Molecular Weight 1100.27
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Formula C54H77N13O12
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Color White to off-white
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Sequence Shortening
YGGFLRKYP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
H2O : 100 mg/mL (90.89 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Minamino N, et al. Beta-neo-endorphin, a new hypothalamic "big" Leu-enkephalin of porcine origin: its purification and the complete amino acid sequence. Biochem Biophys Res Commun. 1981 Apr 15;99(3):864-70. [Content Brief]
[2]. Yang DJ, et al. β-Neoendorphin Enhances Wound Healing by Promoting Cell Migration in Keratinocyte. Molecules. 2020 Oct 12;25(20):4640. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 0.9089 mL | 4.5443 mL | 9.0887 mL | 22.7217 mL |
| 5 mM | 0.1818 mL | 0.9089 mL | 1.8177 mL | 4.5443 mL | |
| 10 mM | 0.0909 mL | 0.4544 mL | 0.9089 mL | 2.2722 mL | |
| 15 mM | 0.0606 mL | 0.3030 mL | 0.6059 mL | 1.5148 mL | |
| 20 mM | 0.0454 mL | 0.2272 mL | 0.4544 mL | 1.1361 mL | |
| 25 mM | 0.0364 mL | 0.1818 mL | 0.3635 mL | 0.9089 mL | |
| 30 mM | 0.0303 mL | 0.1515 mL | 0.3030 mL | 0.7574 mL | |
| 40 mM | 0.0227 mL | 0.1136 mL | 0.2272 mL | 0.5680 mL | |
| 50 mM | 0.0182 mL | 0.0909 mL | 0.1818 mL | 0.4544 mL | |
| 60 mM | 0.0151 mL | 0.0757 mL | 0.1515 mL | 0.3787 mL | |
| 80 mM | 0.0114 mL | 0.0568 mL | 0.1136 mL | 0.2840 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
β-Neo-Endorphin Related Classifications
HY-P3513 Related Classifications
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