1. Neuronal Signaling
  2. AChE

Timosaponin AIII 

Cat. No.: HY-N0810 Purity: 98.88%
Handling Instructions

Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM.

For research use only. We do not sell to patients.

Timosaponin AIII Chemical Structure

Timosaponin AIII Chemical Structure

CAS No. : 41059-79-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 130 In-stock
Estimated Time of Arrival: December 31
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 140 In-stock
Estimated Time of Arrival: December 31
25 mg USD 290 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM.

IC50 & Target

IC50: 35.4 μM (AChE)[1].

In Vitro

Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM[1]. Timosaponin AIII is identified as a major selective cytotoxic activity in BN108, and its selective cytotoxic activity involves inhibition of mTOR, induction of ER stress and protective autophagy[2].

In Vivo

Of the tested steroidal saponins, Timosaponin AIII (TA3) most potently improves memory deficits. Timosaponin AIII increases the scopolamine-induced reduction in step-through latency by 17% (10 mg/kg), 28% (20 mg/kg), and 43% (40 mg/kg). During the acquisition trial, no differences in latent time are observed. Timosaponin AIII (20, 40 mg/kg, p.o.) potently inhibits this reduction of acetylcholine in scopolamine-treated mouse brain. The inhibitory effect of Timosaponin AIII is comparable to that of tacrine, which is used as a positive control[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3497 mL 6.7484 mL 13.4967 mL
5 mM 0.2699 mL 1.3497 mL 2.6993 mL
10 mM 0.1350 mL 0.6748 mL 1.3497 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[1]

Mice[1]
Male ICR mice weighing 28-30 g are used. For the acquisition trial, mice are initially placed in the illuminated compartment and the door between the two compartments is opened 10 s later. Each group contains ten mice. One hour or 5 h before the acquisition trial, mice receive each test agent (e.g., Timosaponin AIII 10, 20 or 40 mg/kg, p.o. ). One hour before the acquisition trial, mice receive tacrine (10 mg/kg, p.o.) as a positive control. Memory impairment is induced by scopolamine treatment (1 mg/kg, i.p.) 0.5 h or 4.5 h after the administration of test agents, tacrine, or 10% Tween 80 solution. Control animals are administered 10% Tween 80 solution alone. Twenty-four hours after the acquisition trial, the mice are again placed in the illuminated compartment for retention trials. The time taken for a mouse to enter the dark compartment after the door opened is measured as the latency time in both acquisition and retention trials, with a maximum of 300 s[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

740.92

Formula

C₃₉H₆₄O₁₃

CAS No.

41059-79-4

SMILES

C[[email protected]@]12[[email protected]]([[email protected]@H]3C)([H])[[email protected]](O[[email protected]]34CC[[email protected]](C)CO4)([H])C[[email protected]@]1([H])[[email protected]@](CC[[email protected]@]5([H])[[email protected]@]6(CC[[email protected]](O[[email protected]@](O[[email protected]](CO)[[email protected]](O)[[email protected]@H]7O)([H])[[email protected]@H]7O[[email protected]]([[email protected]@H]([[email protected]@H](O)[[email protected]@H]8O)O)([H])O[[email protected]@H]8CO)C5)C)([H])[[email protected]]6([H])CC2

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Timosaponin AIII
Cat. No.:
HY-N0810
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Timosaponin AIII

Cat. No.: HY-N0810