Cat. No.: HY-B1234

Octinoxate

(Synonyms: Octyl methoxycinnamate)

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Octinoxate (Octyl methoxycinnamate) is a thyroid hormone receptor agonist, reducing the levels of triiodothyronine (T3) and thyroxine (T4) and transcription levels of genes related to type II deiodinase (deio2) in Japanese Medaka. Octinoxate is commonly used as a safe ultraviolet (UV) filter used in the aquatic environment. Octinoxate inhibits CYP1A1 and CYP1B1 to regulate hyaluronan (HA) (HY-B0633A) metabolism in a PI3K pathway-dependent manner in human keratinocytes. Octinoxate also exhibits an anti-estrogenic and anti-androgenic effect in vitro and in vivo.

For research use only. We do not sell to patients.

Purity: 99.84%
CAS No.: 5466-77-3
Formula: C₁₈H₂₆O₃
Molecular Weight:290.40

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Octinoxate Chemical Structure

CAS No.: 5466-77-3

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Cytochrome P450 Isoforms :

All Estrogen Receptor/ERR Isoforms :

In Vitro

Octinoxate (10 μM, 6 h) potentiates the ability of 6-formylindolo[3,2-b]carbazole (FICZ (HY-12451)) (10 nM, 6 h) and ultraviolet radiation (UVR) to activate the Aryl hydrocarbon Receptor (AhR) by elevating CYP1A1 and CYP1B1 mRNA levels in H1L1.1c2 cells and HaCaT cells^[3].
Octinoxate (10 μM, 24 h) decreases catalytic function of both CYP1A1 and CYP1B1 with IC₅₀ values of 1.0 μM and 586 nM respectively, weaker than α-NF in H1L1.1c2 cells^[3].
Octinoxate (30 μM or 50 μM, 24 h) significantly enhances the mRNA expression of the predominant hyaluronan (HA) metabolic enzyme HAS2 and HYAL1, but inhibits the mRNA expression of AQP3 in HaCaT keratinocytes^[4].
Octinoxate acts as a thyroid hormone receptor agonist in HepG2 cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[3]

Cell Line:

HaCaT cells
Concentration:

10 μM
Incubation Time:

6 h
Result:

Potentiated the ability of FICZ (HY-12451) and UVR to elevate CYP1A1 and CYP1B1 transcript levels in HaCaT cells.

Real Time qPCR^[4]

Cell Line:

HaCaT keratinocytes
Concentration:

30 μM
Incubation Time:

24 h
Result:

Increased the mRNA expression of HAS2 and HYAL1 in HaCaT keratinocytes.

In Vivo

Octinoxate (0, 6.9 μg/kg, 96.0 μg/kg and 395.6 μg/kg, fed with pellets, twice a day for 6 weeks) downregulates dio2 and pax8a gene expression, but has no effect on thrα and thrβ expression in the cranial kidney of rainbow trout^[1].
Octinoxate (30 μM, 120 h) significantly decreases T3 and T4 concentrations in zebrafish larvae, upregulating transcriptions of trh, tshβ, tshr, tg, nis and deio2 genes and downregulating transcriptions of trαa, trβ and tpo genes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female rainbow trout (O. mykiss)^[1]
Dosage:

0, 6.9 μg/kg, 96.0 μg/kg and 395.6 μg/kg
Administration:

fed with pellets, twice a day for 6 weeks
Result:

Significantly downregulated dio2 gene expression in the highest dose group, downregulated pax8a expression in the medium and highest dose group, but not altered thrα and thrβ expression in the cranial kidney.

Chemical Information

CAS No. 5466-77-3
Appearance Liquid (Density: 1.009 g/cm³)
Molecular Weight 290.40
Formula C₁₈H₂₆O₃
Color Light yellow to yellow
SMILES

O=C(OCC(CC)CCCC)/C=C/C1=CC=C(OC)C=C1
Synonyms

Octyl methoxycinnamate
Structure Classification
- Others
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 140 mg/mL (482.09 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4435 mL

17.2176 mL

34.4353 mL

5 mM

0.6887 mL

3.4435 mL

6.8871 mL

10 mM

0.3444 mL

1.7218 mL

3.4435 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: 3.5 mg/mL (12.05 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (35.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)

Solubility: 3.5 mg/mL (12.05 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 3.5 mg/mL (12.05 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (35.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cahova J, et al. Octinoxate as a potential thyroid hormone disruptor - A combination of in vivo and in vitro data. Sci Total Environ. 2023 Jan 15;856(Pt 1):159074. [Content Brief]

[2]. Ka Y, et al. Waterborne exposure to avobenzone and octinoxate induces thyroid endocrine disruption in wild-type and thrαa-/- zebrafish larvae[J]. Ecotoxicology. 2022 Aug;31(6):948-955. [Content Brief]

[3]. Phelan-Dickinson SJ, et al. The UVR Filter Octinoxate Modulates Aryl Hydrocarbon Receptor Signaling in Keratinocytes via Inhibition of CYP1A1 and CYP1B1[J]. Toxicol Sci. 2020 Sep 1;177(1):188-201. [Content Brief]

[4]. Chang KY, et al. Organic ultraviolet filters regulate hyaluronan metabolism in human epidermal keratinocytes through the phosphatidylinositol 3-kinase pathway[J]. Toxicol In Vitro. 2023 Feb;86:105511. [Content Brief]

[5]. Schmutzler C, et al. Endocrine disruptors and the thyroid gland--a combined in vitro and in vivo analysis of potential new biomarkers. Environ Health Perspect. 2007 Dec;115 Suppl 1(Suppl 1):77-83. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol	1 mM	3.4435 mL	17.2176 mL	34.4353 mL	86.0882 mL
	5 mM	0.6887 mL	3.4435 mL	6.8871 mL	17.2176 mL
	10 mM	0.3444 mL	1.7218 mL	3.4435 mL	8.6088 mL
	15 mM	0.2296 mL	1.1478 mL	2.2957 mL	5.7392 mL
	20 mM	0.1722 mL	0.8609 mL	1.7218 mL	4.3044 mL
	25 mM	0.1377 mL	0.6887 mL	1.3774 mL	3.4435 mL
	30 mM	0.1148 mL	0.5739 mL	1.1478 mL	2.8696 mL
	40 mM	0.0861 mL	0.4304 mL	0.8609 mL	2.1522 mL
	50 mM	0.0689 mL	0.3444 mL	0.6887 mL	1.7218 mL
	60 mM	0.0574 mL	0.2870 mL	0.5739 mL	1.4348 mL
	80 mM	0.0430 mL	0.2152 mL	0.4304 mL	1.0761 mL
	100 mM	0.0344 mL	0.1722 mL	0.3444 mL	0.8609 mL

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Octinoxate

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All Cytochrome P450 Isoforms

All Estrogen Receptor/ERR Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Octinoxate Related Classifications

HY-B1234 Related Classifications