2. Cell Cycle/DNA Damage Apoptosis
  3. Deubiquitinase Apoptosis MDM-2/p53
  4. HBX 41108

HBX 41108 is an inhibitor of ubiquitin-specific protease 7 (USP7) with an IC50 of 424 nM. HBX 41108 inhibits USP7-mediated p53 deubiquitination to stabilize p53 and inhibits cancer cell growth. BX 41108 can be used in cancer and diabetes research.

For research use only. We do not sell to patients.

HBX 41108 Chemical Structure

HBX 41108 Chemical Structure

CAS No. : 924296-39-9

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Customer Review

Based on 1 publication(s) in Google Scholar

HBX 41108 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE HBX 41108

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HBX 41108 is an inhibitor of ubiquitin-specific protease 7 (USP7) with an IC50 of 424 nM. HBX 41108 inhibits USP7-mediated p53 deubiquitination to stabilize p53 and inhibits cancer cell growth. BX 41108 can be used in cancer and diabetes research[1][2][3][4].

IC50 & Target

USP7

424 nM (IC50)

hTPH2

 

In Vitro

HBX 41108 (0-3 μM, 24 h) inhibits the proliferation of tumor cells HCT-116, induces P53 dependent apoptosis and does not affect the activity of normal hepatocytes [1].
HBX 41108 (5 μM, 24 h) can inhibit cell cycle arrest and cell senescence induced by USP7 in HUVECs[3].
HBX 41108 (5-25 μM, 48 h) can enhances the hTPH2 promoter activity in RN46A cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HBX 41108 Related Antibodies

Cell Viability Assay[1]

Cell Line: HCT-116, NIH-3T3
Concentration: 0-3 μM
Incubation Time: 24 h
Result: HCT116 colon tumor cells were more sensitive to HBX 41108 (IC50 = 0.27 μmol/L) than normal diploid NIH-3T3 fibroblasts (p53 wild-type) with a 7-fold differential effect (IC50 = 1.77 μmol/L).
In Vivo

HBX 41108 (100 mg/kg/day for 14 days, i.p.) can promote wound healing and reduce blood sugar levels in diabetic rats [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

266.64

Formula

C13H3ClN4O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

N#CC1=C(C#N)N=C2C(C(C3=C2C=CC(Cl)=C3)=O)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (937.59 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7504 mL 18.7519 mL 37.5037 mL
5 mM 0.7501 mL 3.7504 mL 7.5007 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (3.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7504 mL 18.7519 mL 37.5037 mL 93.7594 mL
5 mM 0.7501 mL 3.7504 mL 7.5007 mL 18.7519 mL
10 mM 0.3750 mL 1.8752 mL 3.7504 mL 9.3759 mL
15 mM 0.2500 mL 1.2501 mL 2.5002 mL 6.2506 mL
20 mM 0.1875 mL 0.9376 mL 1.8752 mL 4.6880 mL
25 mM 0.1500 mL 0.7501 mL 1.5001 mL 3.7504 mL
30 mM 0.1250 mL 0.6251 mL 1.2501 mL 3.1253 mL
40 mM 0.0938 mL 0.4688 mL 0.9376 mL 2.3440 mL
50 mM 0.0750 mL 0.3750 mL 0.7501 mL 1.8752 mL
60 mM 0.0625 mL 0.3125 mL 0.6251 mL 1.5627 mL
80 mM 0.0469 mL 0.2344 mL 0.4688 mL 1.1720 mL
100 mM 0.0375 mL 0.1875 mL 0.3750 mL 0.9376 mL
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HBX 41108 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HBX 41108924296-39-9HBX41108HBX-41108DeubiquitinaseApoptosisMDM-2/p53DUBscancerdiabetesHUVECsdiabetic cell modeldiabetic rat modeldiabetic foot ulcerInhibitorinhibitorinhibit

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