HK-2-IN-1
Based on 1 Customer Validation
HK-2-IN-1 is a Hexokinase 2 (HK-2) inhibitor. HK-2-IN-1 exhibits a non-activating effect on human recombinant HK-2 enzyme. HK-2-IN-1 has anti-tumor activity, affecting immune cells in the tumor microenvironment. HK-2-IN-1 has immunomodulatory effects and is potentially useful in the study of cancers such as colon cancer, liver cancer, and breast cancer.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 3114833-48-3
- Formula: C23H16Cl2FN5
- Molecular Weight:452.31
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Kinase Inhibitor Library
HY-L009
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Glycolysis Compound Library
HY-L058
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Anti-Breast Cancer Compound Library
HY-L074
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Anti-Cancer Metabolism Compound Library
HY-L083
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Glucose Metabolism Compound Library
HY-L092
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Anti-Liver Cancer Compound Library
HY-L101
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Anti-Colorectal Cancer Compound Library
HY-L103
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Lactic Acid Metabolic Compound Library
HY-L204
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
HK-2-IN-1 (Compound 20) (50-1000 μM, 48 h) exhibits cytotoxicity against HCT-116, HepG2 ,4T1 and MCF7 cells, with IC50s of 22.04 μM, 21.19 μM, 54.69 μM, 192.3 μM, respectively, and exhibits no cytotoxic effects on healthy fibroblast cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:4T1 tumor cells xenografts BALB/c mice model[1]
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Dosage:5 mg/kg
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Administration:i.p., once a day, 4 weeks
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Result:Inhibited 4T1 tumor growth and metastasis, with two of four mice treated in combination with Cyclophosphamide (CTX) (HY-17420) showing almost complete tumor regression.
Reduced tumor-infiltrating gMDSCs and increased tumor infiltration of CD8 T lymphocytes.
Chemical Information
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CAS No. 3114833-48-3
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Appearance Solid
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Molecular Weight 452.31
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Formula C23H16Cl2FN5
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Color White to off-white
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SMILES
ClC(C=C1Cl)=CC=C1CN(C2=CC(F)=CC=C23)N=C3C4=NC(CC5=CC=CC=C5)=NN4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (110.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2109 mL | 11.0544 mL | 22.1087 mL | 55.2718 mL |
| 5 mM | 0.4422 mL | 2.2109 mL | 4.4217 mL | 11.0544 mL | |
| 10 mM | 0.2211 mL | 1.1054 mL | 2.2109 mL | 5.5272 mL | |
| 15 mM | 0.1474 mL | 0.7370 mL | 1.4739 mL | 3.6848 mL | |
| 20 mM | 0.1105 mL | 0.5527 mL | 1.1054 mL | 2.7636 mL | |
| 25 mM | 0.0884 mL | 0.4422 mL | 0.8843 mL | 2.2109 mL | |
| 30 mM | 0.0737 mL | 0.3685 mL | 0.7370 mL | 1.8424 mL | |
| 40 mM | 0.0553 mL | 0.2764 mL | 0.5527 mL | 1.3818 mL | |
| 50 mM | 0.0442 mL | 0.2211 mL | 0.4422 mL | 1.1054 mL | |
| 60 mM | 0.0368 mL | 0.1842 mL | 0.3685 mL | 0.9212 mL | |
| 80 mM | 0.0276 mL | 0.1382 mL | 0.2764 mL | 0.6909 mL | |
| 100 mM | 0.0221 mL | 0.1105 mL | 0.2211 mL | 0.5527 mL |
HK-2-IN-1 Related Classifications
HY-175035 Related Classifications
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