2. Metabolic Enzyme/Protease
  3. Hexokinase
  4. HK-2-IN-1

HK-2-IN-1 is a Hexokinase 2 (HK-2) inhibitor. HK-2-IN-1 exhibits a non-activating effect on human recombinant HK-2 enzyme. HK-2-IN-1 has anti-tumor activity, affecting immune cells in the tumor microenvironment. HK-2-IN-1 has immunomodulatory effects and is potentially useful in the study of cancers such as colon cancer, liver cancer, and breast cancer.

For research use only. We do not sell to patients.

HK-2-IN-1

HK-2-IN-1 Chemical Structure

CAS No. : 3114833-48-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
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Based on 1 publication(s) in Google Scholar

HK-2-IN-1 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HK-2-IN-1 is a Hexokinase 2 (HK-2) inhibitor. HK-2-IN-1 exhibits a non-activating effect on human recombinant HK-2 enzyme. HK-2-IN-1 has anti-tumor activity, affecting immune cells in the tumor microenvironment. HK-2-IN-1 has immunomodulatory effects and is potentially useful in the study of cancers such as colon cancer, liver cancer, and breast cancer[1].

In Vitro

HK-2-IN-1 (Compound 20) (50-1000 μM, 48 h) exhibits cytotoxicity against HCT-116, HepG2 ,4T1 and MCF7 cells, with IC50s of 22.04 μM, 21.19 μM, 54.69 μM, 192.3 μM, respectively, and exhibits no cytotoxic effects on healthy fibroblast cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HK-2-IN-1 Related Antibodies
In Vivo

HK-2-IN-1 (Compound 20) (5 mg/kg, i.p., once a day, 4 weeks) inhibits tumor growth and metastasis in 4T1 tumor-bearing mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 4T1 tumor cells xenografts BALB/c mice model[1]
Dosage: 5 mg/kg
Administration: i.p., once a day, 4 weeks
Result: Inhibited 4T1 tumor growth and metastasis, with two of four mice treated in combination with Cyclophosphamide (CTX) (HY-17420) showing almost complete tumor regression.
Reduced tumor-infiltrating gMDSCs and increased tumor infiltration of CD8 T lymphocytes.
Molecular Weight

452.31

Formula

C23H16Cl2FN5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

ClC(C=C1Cl)=CC=C1CN(C2=CC(F)=CC=C23)N=C3C4=NC(CC5=CC=CC=C5)=NN4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (110.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2109 mL 11.0544 mL 22.1087 mL
5 mM 0.4422 mL 2.2109 mL 4.4217 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.53 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2109 mL 11.0544 mL 22.1087 mL 55.2718 mL
5 mM 0.4422 mL 2.2109 mL 4.4217 mL 11.0544 mL
10 mM 0.2211 mL 1.1054 mL 2.2109 mL 5.5272 mL
15 mM 0.1474 mL 0.7370 mL 1.4739 mL 3.6848 mL
20 mM 0.1105 mL 0.5527 mL 1.1054 mL 2.7636 mL
25 mM 0.0884 mL 0.4422 mL 0.8843 mL 2.2109 mL
30 mM 0.0737 mL 0.3685 mL 0.7370 mL 1.8424 mL
40 mM 0.0553 mL 0.2764 mL 0.5527 mL 1.3818 mL
50 mM 0.0442 mL 0.2211 mL 0.4422 mL 1.1054 mL
60 mM 0.0368 mL 0.1842 mL 0.3685 mL 0.9212 mL
80 mM 0.0276 mL 0.1382 mL 0.2764 mL 0.6909 mL
100 mM 0.0221 mL 0.1105 mL 0.2211 mL 0.5527 mL
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HK-2-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HK-2-IN-13114833-48-3HexokinaseInhibitor4T1 tumor-bearing miceHCT-116 cellsHepG2 cellscolon cancerliver cancerbreast cancerinhibitorinhibit

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