HKSOX-1

HKSOX-1 and its derivatives (HKSOX-1r and HKSOX-1m) are novel fluorescent probes designed for highly sensitive and selective detection of the superoxide anion radical (O₂^•−) in cellular environments. These probes utilize an aryl trifluoromethanesulfonate group that undergoes O₂^•−-mediated cleavage, releasing a free phenol and emitting fluorescence. They demonstrate excellent specificity and sensitivity across various pH ranges, withstand interference from strong oxidants and reductants typical in cellular contexts. HKSOX-1r, optimized for cellular retention, has been effectively employed in diverse assays including confocal imaging, flow cytometry, and zebrafish embryo studies, highlighting its utility in investigating O₂^•− roles in inflammation, mitochondrial stress, and other physiological processes.

• Data Sheet (272 KB)