HKSOX-1 (5/6-mixture)
Based on 2 publication(s) in Google Scholar
HKSOX-1 (5/6-mixture) is a fluorescent probe which is used for imaging and detection of endogenous superoxide in live cells and in vivo. HKSOX-1 (5/6-mixture) exhibits excellent selectivity and sensitivity towards superoxide anion radical (Ex/Em = 509/534 nm).
For research use only. We do not sell to patients.
- Purity: 98.82%
- Formula: C23H6F10O11S2
- Molecular Weight:712.40
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) HKSOX-1 (5/6-mixture)
More
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM is recommended.
1.2 Preparation of working solution
Dilute to 10 μM with phosphate buffer (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Superoxide staining
2.1 The reaction of HKSOX-1 (10 μM) with O2?? (10 equiv, 100 μM) generated by enzymatic reaction of xanthine (300 μM) and xanthine oxidase (0.01 U/mL) in potassium phosphate buffer (0.1 M, pH 7.4) at 25°C.
2.2 The fluorescence intensity is monitored at the Ex/Em = 509/534 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
534
509
Chemical Information
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Appearance Solid
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Molecular Weight 712.40
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Formula C23H6F10O11S2
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Color Yellow to brown
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SMILES
O=C(C1=CC=C2C(OC3(C2=C1)C4=CC(F)=C(C(F)=C4OC5=C(C(OS(=O)(C(F)(F)F)=O)=C(C=C53)F)F)OS(=O)(C(F)(F)F)=O)=O)O.O=C6OC(C(C7=C8F)=CC(F)=C8O[S](C(F)(F)F)(=O)=O)(C(C6=C9)=CC=C9C(O)=O)C(C(O7)=C%10F)=CC(F)=C%10O[S](C(F)(F)F)(=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Rep
Synthetic lethal CRISPR screen identifies a cancer cell-intrinsic role of PD-L1 in regulation of vulnerability to ferroptosis. [Abstract]2024 Jul 9;43(7):114477. PMID: 38985676 -
mSystems
2023 Dec 21;8(6):e0102623. PMID: 38047647
Solvent & Solubility
DMSO : 110 mg/mL (154.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (288 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4037 mL | 7.0185 mL | 14.0371 mL | 35.0926 mL |
| 5 mM | 0.2807 mL | 1.4037 mL | 2.8074 mL | 7.0185 mL | |
| 10 mM | 0.1404 mL | 0.7019 mL | 1.4037 mL | 3.5093 mL | |
| 15 mM | 0.0936 mL | 0.4679 mL | 0.9358 mL | 2.3395 mL | |
| 20 mM | 0.0702 mL | 0.3509 mL | 0.7019 mL | 1.7546 mL | |
| 25 mM | 0.0561 mL | 0.2807 mL | 0.5615 mL | 1.4037 mL | |
| 30 mM | 0.0468 mL | 0.2340 mL | 0.4679 mL | 1.1698 mL | |
| 40 mM | 0.0351 mL | 0.1755 mL | 0.3509 mL | 0.8773 mL | |
| 50 mM | 0.0281 mL | 0.1404 mL | 0.2807 mL | 0.7019 mL | |
| 60 mM | 0.0234 mL | 0.1170 mL | 0.2340 mL | 0.5849 mL | |
| 80 mM | 0.0175 mL | 0.0877 mL | 0.1755 mL | 0.4387 mL | |
| 100 mM | 0.0140 mL | 0.0702 mL | 0.1404 mL | 0.3509 mL |
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