HKSOX-1 (5/6-mixture)

Name: HKSOX-1 (5/6-mixture)
Brand: MedChemExpress
SKU: HY-130015
Rating: 5.0 (2 reviews)

Cat. No.: HY-130015 Purity: 98.82%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

HKSOX-1 (5/6-mixture) is a fluorescent probe which is used for imaging and detection of endogenous superoxide in live cells and in vivo. HKSOX-1 (5/6-mixture) exhibits excellent selectivity and sensitivity towards superoxide anion radical (Ex/Em = 509/534 nm).

For research use only. We do not sell to patients.

HKSOX-1 (5/6-mixture) Chemical Structure

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE HKSOX-1 (5/6-mixture)

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM is recommended.
1.2 Preparation of working solution
Dilute to 10 μM with phosphate buffer (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Superoxide staining
2.1 The reaction of HKSOX-1 (10 μM) with O₂^?? (10 equiv, 100 μM) generated by enzymatic reaction of xanthine (300 μM) and xanthine oxidase (0.01 U/mL) in potassium phosphate buffer (0.1 M, pH 7.4) at 25°C.
2.2 The fluorescence intensity is monitored at the Ex/Em = 509/534 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

712.40

Formula

C₂₃H₆F₁₀O₁₁S₂

Appearance

Solid

Color

Yellow to brown

Emission (Em)

534

Excitation (Ex)

509

SMILES

O=C(C1=CC=C2C(OC3(C2=C1)C4=CC(F)=C(C(F)=C4OC5=C(C(OS(=O)(C(F)(F)F)=O)=C(C=C53)F)F)OS(=O)(C(F)(F)F)=O)=O)O.O=C6OC(C(C7=C8F)=CC(F)=C8O[S](C(F)(F)F)(=O)=O)(C(C6=C9)=CC=C9C(O)=O)C(C(O7)=C%10F)=CC(F)=C%10O[S](C(F)(F)F)(=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 110 mg/mL (154.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4037 mL	7.0185 mL	14.0371 mL
5 mM	0.2807 mL	1.4037 mL	2.8074 mL
10 mM	0.1404 mL	0.7019 mL	1.4037 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.82%

Select Batch:

Data Sheet (288 KB) SDS (252 KB)

COA (270 KB) HNMR (297 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Hu JJ, et, al. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc. 2015 Jun 3;137(21):6837-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4037 mL	7.0185 mL	14.0371 mL	35.0926 mL
	5 mM	0.2807 mL	1.4037 mL	2.8074 mL	7.0185 mL
	10 mM	0.1404 mL	0.7019 mL	1.4037 mL	3.5093 mL
	15 mM	0.0936 mL	0.4679 mL	0.9358 mL	2.3395 mL
	20 mM	0.0702 mL	0.3509 mL	0.7019 mL	1.7546 mL
	25 mM	0.0561 mL	0.2807 mL	0.5615 mL	1.4037 mL
	30 mM	0.0468 mL	0.2340 mL	0.4679 mL	1.1698 mL
	40 mM	0.0351 mL	0.1755 mL	0.3509 mL	0.8773 mL
	50 mM	0.0281 mL	0.1404 mL	0.2807 mL	0.7019 mL
	60 mM	0.0234 mL	0.1170 mL	0.2340 mL	0.5849 mL
	80 mM	0.0175 mL	0.0877 mL	0.1755 mL	0.4387 mL
	100 mM	0.0140 mL	0.0702 mL	0.1404 mL	0.3509 mL