HNSS peptide
HNSS peptide is a chimeric hybrid peptide composed of the neuroprotective peptide S14G-Humanin and the mitochondria-targeted antioxidant peptide SS31. HNSS peptide is readily cleared in vivo and can be combined with nanocarriers to improve its stability. HNSS can be delivered via the FGFR1-targeting nanocarrier FGL-NP (Cit), ameliorating mitochondrial dysfunction, Aβ deposition, tau hyperphosphorylation, and cholinergic neuronal damage, and alleviating memory deficits in Alzheimer's disease mice. HNSS peptide is applicable for Alzheimer's disease-related research.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C154H255N45O37S2
- 分子量:3393.08
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
[1]|
p-STAT3 |
体外実験
HNSS peptide is efficiently encapsulated into NP (Cit) nanoparticles via electrostatic interactions, with high encapsulation efficiency and drug loading capacity; after encapsulation, its stability in plasma and brain homogenate is significantly enhanced, and it exhibits pH-responsive drug release characteristics, with accelerated HNSS release under acidic pH 5.0 conditions[1].
HNSS peptide (10 μM) most effectively inhibits H2O2-induced intracellular and mitochondrial ROS production in HT22 cells[1].
HNSS peptide (10 μM; 24 h) most effectively restores mitochondrial function in Aβ25-35 (HY-P0128)-treated HT22 cells by upregulating p-STAT3, MTCO1, and MnSOD, and by improving mitochondrial membrane potential, ATP levels, and cell viability[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HT22 cells
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Concentration:25 μM Aβ25-35 (HY-P0128)
10 μM HNSS preparations -
Incubation Time:24 h
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Result:Strongly upregulated the expression of p-STAT3, MTCO1, and MnSOD.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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分子量 3393.08
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分子式 C154H255N45O37S2
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SMILES
O=C([C@H](N)CCCNC(N)=N)N[C@H](C(N[C@H](C(N[C@H](C(NCC(NCC(N[C@H](C(N[C@H](C(N1[C@H](C(N[C@H](C(NCC(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(NCC(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N2[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N)=O)C)=O)CCCNC(N)=N)=O)CCCNC(N)=N)=O)CCCCN)=O)C(C)C)=O)CCC2)=O)CC(C)C)=O)CC(O)=O)=O)[C@H](CC)C)=O)CCC(O)=O)=O)=O)[C@@H](C)O)=O)CC(C)C)=O)CC(C)C)=O)CC(C)C)=O)CC(C)C)=O)CS)=O)CO)=O)CC3=CC=CC=C3)=O)=O)CCCNC(N)=N)=O)CCC1)=O)C)=O)CCSC)=O)=O)=O)CC4=CC=CC=C4)=O)CCCCN)=O)CC5=C(C=C(O)C=C5C)C
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配列
DArg-Tyr(2,6-diMe)-Lys-Phe-Gly-Gly-Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Gly-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala-NH2
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シーケンスの短縮
DArg-Tyr(2,6-diMe)-KFGGMAPRGFSCLLLLTGEIDLPVKRRA-NH2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Mitochondrial membrane-potential and mitochondrial mass staining
Mitochondrial membrane potential staining measures the electrochemical polarization across the mitochondrial inner membrane in live cells using lipophilic cationic fluorescent probes; early rhodamine-based work showed that selective mitochondrial dye accumulation is lost when the mitochondrial transmembrane potential is dissipated. JC-1 reports mitochondrial polarization by shifting from green monomer fluorescence to red J-aggregate fluorescence as dye concentration increases within energized mitochondria; therefore, the red/green fluorescence ratio is used as a relative readout of mitochondrial membrane potential. TMRE or TMRM staining provides a single-channel relative readout because these cationic rhodamine esters accumulate in polarized mitochondria, and lower fluorescence indicates reduced mitochondrial polarization when acquisition and dye-loading conditions are controlled. Mitochondrial mass staining is commonly performed with MitoTracker Green FM or related MitoTracker dyes as
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Alzheimer’s Disease Modeling
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
HNSS peptide 関連分類
HY-P12338 関連分類
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