ZYF0033 (Synonyms: HPK1-IN-22)

Name: ZYF0033
Brand: MedChemExpress
SKU: HY-144088
Rating: 4.5 (1 reviews)

Cat. No.: HY-144088 Purity: 98.26%: FAQs
Data Sheet SDS COA Handling Instructions

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ZYF0033 is effective in inhibiting hematopoietic progenitor cells HPK1, basically inhibiting MBP protein oxidation IC₅₀ 10 nM . ZYF0033 promotes anti-cancer immune response, lowers SLP76 (acid 376) oxidation. ZYF0033 Suppression 4T-1 Small mouse model with the same underlying cause, medium bulge growth length expansion DC, NK 细细和 CD107a^{+ CD8⁺ T Cells, PD-1⁺CD8⁺ T Cells, TIM-3⁺CD8⁺ T Cells LAG3< sup>+}CD8⁺ T Cellular immersion decreases.

For research use only. We do not sell to patients.

ZYF0033 Chemical Structure

CAS No. : 2380300-79-6

Size	Price	Stock	Quantity
1 mg	USD 129	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 323	Get quote	Estimated Time of Arrival: December 31
10 mg	USD 549	Get quote	Estimated Time of Arrival: December 31
25 mg	USD 1153	Get quote	Estimated Time of Arrival: December 31
50 mg	USD 1960	Get quote	Estimated Time of Arrival: December 31
100 mg	USD 3333	Get quote	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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MAP4K2/GCK MAP4K3/GLK MAP4K4/HGK MAP4K5/KHS1 MAP4K6/MINK1 MAP4K7/TNIK MAP4K1/HPK1

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

HPK1

<10 nM (IC₅₀)

In Vitro

ZYF0033 (100 nM; 24 h) reduces phosphorylation of SLP76 (serine 376), a key biomarker of T cell and HPK1 activation. ZYF0033 causes HPK1 inhibition, increased proliferation of CD4+ and CD8+ T cells, and IFN-γ secretion^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The maximum tolerated dose of ZYF0033 exceeds 50 mg/kg (daily; po) and exceeds 120 mg/kg in a 6-day toxicity study. In the 4T-1 syngeneic mouse model, ZYF0033 regulates immune cell subsets, increases DC and NK cell infiltration, and promotes anti-cancer immune response^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

462.61

Formula

C₂₆H₃₀N₄O₂S

CAS No.

2380300-79-6

Appearance

Solid

Color

White to off-white

SMILES

OC(C)(C)C#CC1=CC=CC(COC2=C(N)N=CC(C3=CN=C(C4CCN(CC4)C)S3)=C2)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (216.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1616 mL	10.8082 mL	21.6165 mL
5 mM	0.4323 mL	2.1616 mL	4.3233 mL
10 mM	0.2162 mL	1.0808 mL	2.1616 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.26%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (245 KB) HNMR (145 KB) RP-HPLC (253 KB) LCMS (109 KB)

Handling Instructions (2659 KB)

References

[1]. Si J, et al. Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. Cancer Cell. 2020 Oct 12;38(4):551-566.e11. [Content Brief]

[2]. Jingwen Si, et al. Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. Cancer Cell. 2020 Oct 12;38(4):551-566.e11.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1616 mL	10.8082 mL	21.6165 mL	54.0412 mL
	5 mM	0.4323 mL	2.1616 mL	4.3233 mL	10.8082 mL
	10 mM	0.2162 mL	1.0808 mL	2.1616 mL	5.4041 mL
	15 mM	0.1441 mL	0.7205 mL	1.4411 mL	3.6027 mL
	20 mM	0.1081 mL	0.5404 mL	1.0808 mL	2.7021 mL
	25 mM	0.0865 mL	0.4323 mL	0.8647 mL	2.1616 mL
	30 mM	0.0721 mL	0.3603 mL	0.7205 mL	1.8014 mL
	40 mM	0.0540 mL	0.2702 mL	0.5404 mL	1.3510 mL
	50 mM	0.0432 mL	0.2162 mL	0.4323 mL	1.0808 mL
	60 mM	0.0360 mL	0.1801 mL	0.3603 mL	0.9007 mL
	80 mM	0.0270 mL	0.1351 mL	0.2702 mL	0.6755 mL
	100 mM	0.0216 mL	0.1081 mL	0.2162 mL	0.5404 mL

ZYF0033 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ZYF0033 (Synonyms: HPK1-IN-22)

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ZYF0033 Related Antibodies

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Biological Activity

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Complete Stock Solution Preparation Table

ZYF0033 Related Classifications

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