2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK
  3. EGFR
  4. HW12

HW12 is a scrambled peptide control with no EGFR ligand activity. HW12 shows low cellular uptake levels in EGFR-positive cells. HW12 exerts weak growth inhibitory effects on pancreatic cancer. HW12 serves as a scrambled peptide control for EGFR-targeted GE11 (HY-P10128)-conjugated mixed micelles. HW12 can be used in pancreatic cancer research.

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HW12

HW12 Chemical Structure

CAS No. : 875142-27-1

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HW12 Related Antibodies

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Description

HW12 is a scrambled peptide control with no EGFR ligand activity. HW12 shows low cellular uptake levels in EGFR-positive cells. HW12 exerts weak growth inhibitory effects on pancreatic cancer. HW12 serves as a scrambled peptide control for EGFR-targeted GE11 (HY-P10128)-conjugated mixed micelles. HW12 can be used in pancreatic cancer research[1].

In Vitro

The HW12 peptide can be successfully conjugated with MAL-PEG-PCD via a thiol-maleimide addition reaction to form HW12-PEG-PCD, and this result is confirmed by 1H NMR analysis[1].
The cellular uptake of FC-labeled HW12-linked mixed micelles (12 h) by MIA PaCa-2 cells is significantly lower than that of GE11-conjugated mixed micelles, indicating that HW12 cannot mediate EGFR-specific cellular uptake[1].
HW12-linked mixed micelles (60-500 nM GEM; 72 h) exhibit lower cytotoxicity against MIA PaCa-2 cells than GE11-linked mixed micelles, with an IC50 of 126.58 nM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HW12 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: MIA PaCa-2 pancreatic cancer cells
Concentration: HW12-linked mixed micelles with GEM concentrations of 60, 500 nM
Incubation Time: 72 h
Result: Showed lower cytotoxicity than GE11-linked mixed micelles and unmodified micelles at all tested GEM concentrations.
Reached an IC50 value of 126.58 nM, which was 1.3-fold higher than the IC50 of GE11-linked mixed micelles (95.32 nM) and 1.1-fold higher than the IC50 of unmodified micelles (116.91 nM).
In Vivo

HW12-linked mixed micelles (40 mg/kg equivalent dose of gemcitabine; i.v.; once weekly; 3 weeks) deliver gemcitabine but show minimal orthotopic pancreatic tumor growth inhibition in athymic nude mice, with significantly lower efficacy than EGFR-targeted GE11-linked mixed micelles[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: athymic nude mice (6-8 week-old)[1]
Dosage: 40 mg/kg equivalent dose of gemcitabine
Administration: i.v.; once weekly; 3 weeks
Result: Showed minimal tumor growth inhibition compared to GE11-linked mixed micelles.
Resulted in significantly higher tumor bioluminescence, volume, and weight than in GE11-linked mixed micelle-treated mice.
Induced more Ki-67 staining, less cleaved caspase-3 staining, fewer necrotic tumor areas, less TUNEL-positive tumor and endothelial cell apoptosis, and higher tumor microvessel density compared to GE11-linked mixed micelles.
Caused no abnormal body weight changes.
Led to no pathological changes detected in major organs (heart, liver, kidney).
Molecular Weight

1492.59

Formula

C72H89N19O17
CAS No.
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References
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HW12 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HW12875142-27-1HW 12HW-12EGFREpidermal growth factor receptorErbB-1HER1athymic nude micepolymeric mixed micellesgemcitabineGE11-linked mixed micellestumor vasculatureorthotopic pancreatic tumorMIA PaCa-2 cellspancreatic cancer cellspancreatic cancerInhibitorinhibitorinhibit

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