HYNIC-H6F
HYNIC-H6F is a SPECT imaging probe with binding specificity for human epidermal growth factor receptor 2 (HER2) domain II (IC50 = 11 nM). HYNIC-H6F accumulates in HER2-positive breast cancer xenografts via receptor-mediated uptake, while shows low uptake in HER2-negative breast cancer xenografts. HYNIC-H6F enables non-invasive detection of HER2-positive breast cancer in mouse models and allows evaluation of HER2 expression levels without blocking interference. HYNIC-H6F can be used in breast cancer-related research.
For research use only. We do not sell to patients.
- Formula: C64H82N14O13
- Molecular Weight:1255.42
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
All EGFR Isoforms
More
Biological Activity
Description
IC50 & Target
[1]|
HER2 11 nM (IC50) |
In Vitro
HYNIC-H6F (11 nM; 2 h) binds specifically to HER2 on MDA-MB-453 cells with an IC50 of 11 nM, with minimal impact on binding affinity from HYNIC conjugation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
99mTc-HYNIC-H6F (0.37-37 MBq; i.v. via tail vein; single dose) exhibits minimal accumulation in HER2-negative MDA-MB-231 breast cancer xenografts, with a biodistribution-quantified uptake of 0.99 %ID/g at 30 min after injection that is significantly lower than uptake in HER2-positive tumors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
Molecular Weight 1255.42
-
Formula C64H82N14O13
-
Sequence
{HYNIC}-Tyr-Leu-Phe-Phe-Val-Phe-Glu-Arg
-
Sequence Shortening
{HYNIC}-YLFFVFER
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HYNIC-H6F Related Classifications
HY-P10792A Related Classifications
Powered by Bioz