Mcl-1-IN-23

Mcl-1-IN-23 (Compound 53) reversibly binds to purified Mcl-1 protein at a 1:1 ratio, with a K_d value of 0.31 μM, and the binding process is jointly mediated by enthalpy and entropy^[1].
Mcl-1-IN-23 serves as a long-acting ligand for purified GST-Mcl-1 protein, with a K_d value of 1.89 μM, and exhibits slow binding and dissociation kinetic processes^[1].
Mcl-1-IN-23 (48 h) potently inhibits the proliferation of Mcl-1-sensitive Molm-13 cells with an IC₅₀ of 0.45 μM; it remains active against Venetoclax (HY-15531)-resistant Molm-13_VenR cells with an IC₅₀ of 1.16 μM; and it exhibits low micromolar activity against other hematological tumor cell lines^[1].
Mcl-1-IN-23 (0-10 μM; 4 h) dose-dependently displaces the pro-apoptotic proteins Bim and Bak from Mcl-1 in Molm-13 cells, indicating that it can target and inhibit Mcl-1^[1].
Mcl-1-IN-23 (0.3-3 μM; 48 h) induces apoptosis in Molm-13 cells in a dose-dependent manner^[1].
Mcl-1-IN-23 (0-10 μM; 24 h) activates the apoptotic pathway in Molm-13 cells via concentration-dependent cleavage of PARP and Caspase-3, without affecting the expression levels of Bcl-2 or Bcl-xL, confirming that the apoptosis it induces is dependent on Mcl-1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.