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Cat. No.: HY-B0307A

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Idoxuridine hydrate

(Synonyms: 5-Iodo-2′-deoxyuridine hydrate; 5-IUdR hydrate; IdUrd hydrate)

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

Idoxuridine (5-Iodo-2′-deoxyuridine, 5-IUdR, IdUrd) hydrate is an iodinated thymidine analogue that competitively inhibits phosphorylases. Idoxuridine can inhibit viral activity, particularly viral eye infections, including herpes simplex keratitis, by inhibiting DNA polymerase and affecting viral replication. Idoxuridine against feline herpesvirus has the IC₅₀ value of 4.3 μM.

For research use only. We do not sell to patients.

CAS No.: 17140-71-5
Formula: C₉H₁₃IN₂O₆
Molecular Weight:372.11

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

Idoxuridine hydrate Chemical Structure

CAS No.: 17140-71-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (12)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

DNA Polymerase

All DNA/RNA Synthesis Isoforms :

In Vitro

Idoxuridine (2-10 μM, 72 hours) hydrate has the IC₅₀ value of 4.3 μM of antiviral^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

Crandell-Reese feline kidney (CRFK) cells
Concentration:

2-10 μM
Incubation Time:

72 hours
Result:

Showed the IC₅₀ value of 4.3 μM.

Cell Cytotoxicity Assay^[1]

Cell Line:

Crandell-Reese feline kidney (CRFK) cells
Concentration:

5-50 μM
Incubation Time:

72 hours
Result:

Reduced by 10.8% relatively in CRFK cells.

In Vivo

Idoxuridine (intraperitoneal injection, 50-200 mg/kg, 3 times, 3 hours interval) hydrate can stimulate the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in C3HeB/FeJ female and male mice and A/J male mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C3HeB/FeJ female and male mice and A/J male mice, aged 2 to 4 months^[2]
Dosage:

50-200 mg/kg
Administration:

Intraperitoneal injection, 3 times, 3 hours interval
Result:

Stimulated the production of hemolysin plaque-forming cells (HPFC) to sheep red blood cells (SRBC) in the dose range of 50-200 mg/kg.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 17140-71-5
Molecular Weight 372.11
Formula C₉H₁₃IN₂O₆
SMILES

OC[C@@H]1[C@H](C[C@H](N2C(NC(C(I)=C2)=O)=O)O1)O.O
Synonyms

5-Iodo-2′-deoxyuridine hydrate; 5-IUdR hydrate; IdUrd hydrate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (12)

All Cell Imaging/StainingCell Proliferation/Viability AssayBio/Physico-chemical Assay

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Journal Impact Factor

Cell Death Differ

NKAP acts with HDAC3 to prevent R-loop associated genome instability. [Abstract]

2023 Jul;30(7):1811-1828. PMID: 37322264
Adv Sci (Weinh)

DMAP1 Deficiency Suppresses Lung Cancer Progression by Destabilizing Replication Fork and Activating IFN Signaling-Mediated Anti-tumor Immunity. [Abstract]

2026 Jun;13(33):e17634. PMID: 41904944
Cell Death Dis

PHF20 stabilizes the GAS7-F-actin axis to drive DNA damage repair and chemoresistance in cutaneous squamous cell carcinoma. [Abstract]

2026 May 29. PMID: 42215448
Sci China Life Sci

SETD3-mediated histidine methylation of MCM7 regulates DNA replication by facilitating chromatin loading of MCM. [Abstract]

2025 Mar;68(3):793-808. PMID: 39455502
Genes Dis

2025 Oct 21.
Emerg Microbes Infect

The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China. [Abstract]

2023 Dec;12(1):2208682. PMID: 37128898

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(1):2208682. [Abstract]

The cytotoxicity test results of the drug showed that the CC₅₀ of Tecovinimat was 21.95 μM, the CC₅₀ of Idoxuridine was 18.33 μM, the CC₅₀ of Brincidofovir was 17.87 μM, and the CC₅₀ of Cidovir > 200 μM.
Oncogene

Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cells. [Abstract]

2020 Apr;39(14):2905-2920. PMID: 32029902

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Apr;39(14):2905-2920. [Abstract]

Representative images of CIdU and Idoxuridine (IdU, 25 μM, 30 μM) positive PC-3 cells (scale bar: 25 μm).

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Apr;39(14):2905-2920. [Abstract]

The percentage of CIdU and Idoxuridine (IdU, 25 μM, 30 μM) positive cells in three wells.
Virology

5-Bromo-2'-deoxyuridine inhibits African swine fever virus (ASFV) replication via interfering viral DNA replication and suppressing the formation of viral factories. [Abstract]

2024 Sep 12:600:110237. PMID: 39288610
University of North Carolina at Chapel Hill

2025.
bioRxiv

AURKA inhibition amplifies DNA replication stress to foster WEE1 kinase dependency and synergistic antitumor effects with WEE1 inhibition in cancers. [Abstract]

2025 May 29:2025.05.28.656693. PMID: 40501675
bioRxiv

The RING Finger E3 Ligase RNF25 Protects DNA Replication Forks Independently of its Canonical Roles in Ubiquitin Signaling. [Abstract]

2025 Jan 9:2025.01.09.632184. PMID: 39829812

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jan 9:2025.01.09.632184. [Abstract]

Bottom: RNF25^+/+ and RNF25^−/− Pa02C and Pa03C cells were labeled with CldU and Idoxuridine (IdU) (250 μM) and fork speed was calculated by dividing tract lengths by total pulse time.

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jan 9:2025.01.09.632184. [Abstract]

Top: Schematic of fork degradation assays. Bottom: RNF25^+/+ and RNF25^−/− Pa02C and Pa03C cells were labeled with CldU and Idoxuridine (IdU) (250 μM), then treated with HU to induce fork stalling. The ratio of IdU to CldU tracts was used as a readout of fork degradation.
SSRN

2023 Mar 20.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. ]David J Maggs, et al. In vitro efficacy of ganciclovir, cidofovir, penciclovir, foscarnet, idoxuridine, and acyclovir against feline herpesvirus type-1. Am J Vet Res. 2004 Apr;65(4):399-403. [Content Brief]

[2]. D E Griswold, et al. Stimulation of hemolysin plaque-forming cells by idoxuridine. Cancer Res. 1975 Jan;35(1):88-92. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Idoxuridine hydrate

12 Cited Publications

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (12)
Purity & Documentation
References

1/16
1/16

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jan 9:2025.01.09.632184. [Abstract]

Customer Validation
Bottom: RNF25^+/+ and RNF25^−/− Pa02C and Pa03C cells were labeled with CldU and Idoxuridine (IdU) (250 μM) and fork speed was calculated by dividing tract lengths by total pulse time.
1/16

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jan 9:2025.01.09.632184. [Abstract]

Customer Validation
Top: Schematic of fork degradation assays. Bottom: RNF25^+/+ and RNF25^−/− Pa02C and Pa03C cells were labeled with CldU and Idoxuridine (IdU) (250 μM), then treated with HU to induce fork stalling. The ratio of IdU to CldU tracts was used as a readout of fork degradation.
1/16

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(1):2208682. [Abstract]

Customer Validation
The cytotoxicity test results of the drug showed that the CC₅₀ of Tecovinimat was 21.95 μM, the CC₅₀ of Idoxuridine was 18.33 μM, the CC₅₀ of Brincidofovir was 17.87 μM, and the CC₅₀ of Cidovir > 200 μM.
1/16

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Apr;39(14):2905-2920. [Abstract]

Customer Validation
Representative images of CIdU and Idoxuridine (IdU, 25 μM, 30 μM) positive PC-3 cells (scale bar: 25 μm).
1/16

Idoxuridine hydrate purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Apr;39(14):2905-2920. [Abstract]

Customer Validation
The percentage of CIdU and Idoxuridine (IdU, 25 μM, 30 μM) positive cells in three wells.

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Idoxuridine hydrate

Publications Citing Use of MedChemExpress (MCE) Idoxuridine hydrate

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Publications (12)

Purity & Documentation

References

Calculators

Help & FAQs

Idoxuridine hydrate Related Classifications

HY-B0307A Related Classifications