IM176OUT05 (Synonyms: 2-Me-Phen hydrochloride; 2-Me-Phenformin hydrochloride)

Name: IM176OUT05
Brand: MedChemExpress
SKU: HY-125283
Rating: 4.5 (1 reviews)

Cat. No.: HY-125283 Purity: 99.43%: Data Sheet
COA

SDS
Handling Instructions
FAQs
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IM176OUT05 (2-Me-Phen hydrochloride), a biguanide, is a mitochondrial OXPHOS inhibitor. IM176OUT0 inhibits mitochondrial electron transport chain (ETC) activity with an IC₅₀ of 3.2 μM. IM176OUT05 activates stem cell metabolism, promotes hair regrowth and increases stemness induction and maintenance during the pluripotent stem cell generation process.

For research use only. We do not sell to patients.

IM176OUT05 Chemical Structure

CAS No. : 1643659-96-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

IM176OUT05 (0.1-10 μM; 24 hour) inhibits mitochondrial function and reduces the oxygen consumption rate (OCR) as a surrogate of mitochondrial electron transport chain (ETC) activity with an IC₅₀ of 3.2 μM^[1].
IM176OUT05 (10 μM; 6 days) improves the acquisition and maintenance of stem cell pluripotency of mouse and human induced pluripotent stem cells (iPSCs)^[1].
IM176OUT05 (10 and 100 nM) facilitates the transition of glycolytic metabolism and induces of the expression of glycolysis-related genes and ETC complex enzymes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

IM176OUT05 (200 μL, 1%; apply to the depilated area, once daily) promotes hair regrowth in mice by stimulating the progression of the hair follicle cycle to the anagen phase and increasing the hair follicle number^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	7-week-old C57BL/6 mice^[1]
Dosage:	200 μL, 1%
Administration:	Apply to the depilated area; 200 μL, 1%, once daily
Result:	Strongly promoted the hair regrowth, especially in female mice.

Molecular Weight

255.75

Formula

C₁₁H₁₈ClN₅

CAS No.

1643659-96-4

Appearance

Solid

Color

White to off-white

SMILES

NC(NC(NCCC1=CC=CC=C1C)=N)=N.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (391.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9101 mL	19.5503 mL	39.1007 mL
5 mM	0.7820 mL	3.9101 mL	7.8201 mL
10 mM	0.3910 mL	1.9550 mL	3.9101 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.43%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (245 KB) HNMR (251 KB) RP-HPLC (250 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Son MJ, et al. A novel and safe small molecule enhances hair follicle regeneration by facilitating metabolic reprogramming. Exp Mol Med. 2018 Dec 6;50(12):1-15.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9101 mL	19.5503 mL	39.1007 mL	97.7517 mL
	5 mM	0.7820 mL	3.9101 mL	7.8201 mL	19.5503 mL
	10 mM	0.3910 mL	1.9550 mL	3.9101 mL	9.7752 mL
	15 mM	0.2607 mL	1.3034 mL	2.6067 mL	6.5168 mL
	20 mM	0.1955 mL	0.9775 mL	1.9550 mL	4.8876 mL
	25 mM	0.1564 mL	0.7820 mL	1.5640 mL	3.9101 mL
	30 mM	0.1303 mL	0.6517 mL	1.3034 mL	3.2584 mL
	40 mM	0.0978 mL	0.4888 mL	0.9775 mL	2.4438 mL
	50 mM	0.0782 mL	0.3910 mL	0.7820 mL	1.9550 mL
	60 mM	0.0652 mL	0.3258 mL	0.6517 mL	1.6292 mL
	80 mM	0.0489 mL	0.2444 mL	0.4888 mL	1.2219 mL
	100 mM	0.0391 mL	0.1955 mL	0.3910 mL	0.9775 mL

IM176OUT05 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IM176OUT051643659-96-42-Me-Phen2-Me-PhenforminMitochondrial MetabolismOxidative Phosphorylationhigh solubilitybiguanidesmall moleculeETCOCRmitochondrial functioniPSCInhibitorinhibitorinhibit

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IM176OUT05 (Synonyms: 2-Me-Phen hydrochloride; 2-Me-Phenformin hydrochloride)

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IM176OUT05 Related Antibodies

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Biological Activity

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Complete Stock Solution Preparation Table

IM176OUT05 Related Classifications

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