IM176OUT05
Based on 1 Customer Validation
IM176OUT05 (2-Me-Phen hydrochloride), a biguanide, is a mitochondrial OXPHOS inhibitor. IM176OUT0 inhibits mitochondrial electron transport chain (ETC) activity with an IC50 of 3.2 μM. IM176OUT05 activates stem cell metabolism, promotes hair regrowth and increases stemness induction and maintenance during the pluripotent stem cell generation process.
For research use only. We do not sell to patients.
- Purity: 99.43%
- CAS No.: 1643659-96-4
- Formula: C11H18ClN5
- Molecular Weight:255.75
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Stem Cell Signaling Compound Library
HY-L017
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Anti-Aging Compound Library
HY-L034
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Reprogramming Compound Library
HY-L039
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Glycolysis Compound Library
HY-L058
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Glutamine Metabolism Compound Library
HY-L064
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Anti-Cancer Metabolism Compound Library
HY-L083
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Anti-Parkinson's Disease Compound Library
HY-L085
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Neurodegenerative Disease-related Compound Library
HY-L086
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Mitochondria-Targeted Compound Library
HY-L089
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Glucose Metabolism Compound Library
HY-L092
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Cuproptosis Compound Library
HY-L133
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Cancer Stem Cells Compound Library
HY-L135
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Mitochondrial Protection Compound Library
HY-L144
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IM176OUT05 (0.1-10 μM; 24 hour) inhibits mitochondrial function and reduces the oxygen consumption rate (OCR) as a surrogate of mitochondrial electron transport chain (ETC) activity with an IC50 of 3.2 μM[1]. IM176OUT05 (10 μM; 6 days) improves the acquisition and maintenance of stem cell pluripotency of mouse and human induced pluripotent stem cells (iPSCs)[1]. IM176OUT05 (10 and 100 nM) facilitates the transition of glycolytic metabolism and induces of the expression of glycolysis-related genes and ETC complex enzymes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:7-week-old C57BL/6 mice[1]
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Dosage:200 μL, 1%
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Administration:Apply to the depilated area; 200 μL, 1%, once daily
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Result:Strongly promoted the hair regrowth, especially in female mice.
Chemical Information
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CAS No. 1643659-96-4
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Appearance Solid
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Molecular Weight 255.75
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Formula C11H18ClN5
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Color White to off-white
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SMILES
NC(NC(NCCC1=CC=CC=C1C)=N)=N.Cl
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Synonyms
2-Me-Phen hydrochloride; 2-Me-Phenformin hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (391.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9101 mL | 19.5503 mL | 39.1007 mL | 97.7517 mL |
| 5 mM | 0.7820 mL | 3.9101 mL | 7.8201 mL | 19.5503 mL | |
| 10 mM | 0.3910 mL | 1.9550 mL | 3.9101 mL | 9.7752 mL | |
| 15 mM | 0.2607 mL | 1.3034 mL | 2.6067 mL | 6.5168 mL | |
| 20 mM | 0.1955 mL | 0.9775 mL | 1.9550 mL | 4.8876 mL | |
| 25 mM | 0.1564 mL | 0.7820 mL | 1.5640 mL | 3.9101 mL | |
| 30 mM | 0.1303 mL | 0.6517 mL | 1.3034 mL | 3.2584 mL | |
| 40 mM | 0.0978 mL | 0.4888 mL | 0.9775 mL | 2.4438 mL | |
| 50 mM | 0.0782 mL | 0.3910 mL | 0.7820 mL | 1.9550 mL | |
| 60 mM | 0.0652 mL | 0.3258 mL | 0.6517 mL | 1.6292 mL | |
| 80 mM | 0.0489 mL | 0.2444 mL | 0.4888 mL | 1.2219 mL | |
| 100 mM | 0.0391 mL | 0.1955 mL | 0.3910 mL | 0.9775 mL |
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