IMHDPA-4-OH
IMHDPA-4-OH is an mTORC1 inhibitor and autophagy inducer. IMHDPA-4-OH inhibits mTORC1 signaling and reduces p70S6K and p4EBP1 phosphorylation, thereby relieving the autophagy blockade. IMHDPA-4-OH interferes with cholesterol metabolism and induces cell cycle arrest at G0. IMHDPA-4-OH can be used for research on cervical cancer.
For research use only. We do not sell to patients.
- CAS No.: 3105699-76-8
- Formula: C17H20N6O
- Molecular Weight:324.38
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | GI50 |
2.96 nM
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Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay.
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42571760 |
In Vitro
IMHDPA-4-OH (Compound 39) (72 h) exhibits potent antiproliferative activity against HeLa cells with a GI50 of 2.96 nM[1].
IMHDPA-4-OH (5-20 nM; two weeks) suppresses clonogenic growth of HeLa cells in a dose-dependent manner[1].
IMHDPA-4-OH (20-40 nM; 24 h) inhibits the migration and invasion of HeLa cells[1].
IMHDPA-4-OH (80 nM; 12 days) suppresses the growth of 3D HeLa tumor spheroids[1].
IMHDPA-4-OH (20 nM; 48 h) induces weak senescence characteristics in HeLa cells[1].
IMHDPA-4-OH (20-80 nM) modulates the expression of senescence hallmark proteins in HeLa cells[1].
IMHDPA-4-OH (40 nM; 48 h) induces the formation of autophagosomes in HeLa cells[1].
IMHDPA-4-OH (20-80 nM) decreases cleaved PARP and activates autophagy in HeLa cells[1].
IMHDPA-4-OH (20-80 nM) inhibits mTORC1 signaling in HeLa cells[1].
IMHDPA-4-OH (10-40 nM; 12-48 h) induces cell cycle arrest in the G0/G1 phase in a time- and dose-dependent manner in HeLa cells[1].
IMHDPA-4-OH (10-40 nM; 12-48 h) increases the proportion of Annexin V-positive HeLa cells in a time- and dose-dependent manner[1].
IMHDPA-4-OH (2 μM; 12 h) does not induce ROS accumulation in HeLa cells[1].
IMHDPA-4-OH (20-80 nM; 48 h) induces bulk macroautophagy, rather than selective mitophagy, in HeLa cells[1].
IMHDPA-4-OH (20 nM; 48 h) disrupts cholesterol metabolism in HeLa cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa
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Concentration:5 nM; 10 nM; 20 nM
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Incubation Time:two weeks
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Result:Resulted in a dose-dependent reduction in both colony number and size compared to control cells.
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Cell Line:HeLa
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Concentration:20 nM; 40 nM
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Incubation Time:24 h
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Result:Reduced the migration and invasion abilities of HeLa cells, as the quantity of cells migrating across the transwell or invading the Matrigel-coated filter significantly decreased compared to controls.
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Cell Line:HeLa
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Concentration:10-40 nM (dose-dependence); 20 nM (time-course)
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Incubation Time:24 h (dose-dependence); 12-48 h (time-course)
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Result:Increased the proportion of cells in the G0/G1 phase to 65.17%, 70.81%, and 77.25% at 10, 20, and 40 nM respectively, compared to 59.45% in the non-treated group.
Prolonging treatment with 20 nM increased G0/G1 phase cells to 73.73%, 80.56%, and 84.34% at 12, 24, and 48 h, compared to 65.64% at 0 h.
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Cell Line:HeLa
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Concentration:10-40 nM (dose-dependence); 20 nM (time-course)
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Incubation Time:24 h (dose-dependence); 12-48 h (time-course)
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Result:The proportion of Annexin V-positive cells increased from 8.52% in the control group to 13.15%, 32.7%, and 48.5% following treatment with 10, 20, and 40 nM IMHDPA-4-OH, respectively.
Prolonging treatment with 20 nM increased the percentage of Annexin V-positive cells from 8.54% to 13.03%, 25.34%, and 58.4% after 12, 24, and 48 h, respectively.
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Cell Line:HeLa
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Concentration:20 nM; 40 nM; 80 nM
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Incubation Time:48 h
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Result:Showed a dose-dependent reduction in p62.
Neither TOM20 nor COX IV exhibited appreciable changes following IMHDPA-4-OH treatment.
Chemical Information
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CAS No. 3105699-76-8
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Molecular Weight 324.38
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Formula C17H20N6O
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SMILES
OC1=CC=CC2=C1C(/C=N/NC3=NC=NC(N(CC)CC)=C3)=CN2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
IMHDPA-4-OH Related Classifications
HY-189376 Related Classifications
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