XM462
Based on 1 Customer Validation
XM462 is a dihydroceramide desaturase inhibitor. XM462 produced a mixed-type inhibition (Ki=2 μM) in vitro. XM462 has dihydroceramide desaturase inhibition both in vitro and in cultured cells with IC50 values of 8.2 and 0.78 μM, respectively. XM462 can be used for the research of tumor.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 1045857-53-1
- Formula: C25H51NO3S
- Molecular Weight:445.74
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50: 8.2 μM (dihydroceramide desaturase in vitro); 0.78 μM (dihydroceramide desaturase in cultured cells)[1].
Chemical Information
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CAS No. 1045857-53-1
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Appearance Solid
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Molecular Weight 445.74
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Formula C25H51NO3S
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Color White to off-white
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SMILES
CCCCCCCC(N[C@@H](CO)[C@H](O)CSCCCCCCCCCCCCC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (224.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Munoz-Olaya JM, et al. Synthesis and biological activity of a novel inhibitor of dihydroceramide desaturase. ChemMedChem. 2008 Jun;3(6):946-53. [Content Brief]
[2]. Camacho L, et al. 3-Deoxy-3,4-dehydro analogs of XM462. Preparation and activity on sphingolipid metabolism and cell fate. Bioorg Med Chem. 2012 May 15;20(10):3173-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2435 mL | 11.2173 mL | 22.4346 mL | 56.0865 mL |
| 5 mM | 0.4487 mL | 2.2435 mL | 4.4869 mL | 11.2173 mL | |
| 10 mM | 0.2243 mL | 1.1217 mL | 2.2435 mL | 5.6087 mL | |
| 15 mM | 0.1496 mL | 0.7478 mL | 1.4956 mL | 3.7391 mL | |
| 20 mM | 0.1122 mL | 0.5609 mL | 1.1217 mL | 2.8043 mL | |
| 25 mM | 0.0897 mL | 0.4487 mL | 0.8974 mL | 2.2435 mL | |
| 30 mM | 0.0748 mL | 0.3739 mL | 0.7478 mL | 1.8696 mL | |
| 40 mM | 0.0561 mL | 0.2804 mL | 0.5609 mL | 1.4022 mL | |
| 50 mM | 0.0449 mL | 0.2243 mL | 0.4487 mL | 1.1217 mL | |
| 60 mM | 0.0374 mL | 0.1870 mL | 0.3739 mL | 0.9348 mL | |
| 80 mM | 0.0280 mL | 0.1402 mL | 0.2804 mL | 0.7011 mL | |
| 100 mM | 0.0224 mL | 0.1122 mL | 0.2243 mL | 0.5609 mL |
XM462 Related Classifications
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