SEA0400
Based on 20 publication(s) in Google Scholar
SEA0400 is a novel and selective inhibitor of the Na+-Ca2+ exchanger (NCX), inhibiting Na+-dependent Ca2+ uptake in cultured neurons, astrocytes, and microglia with IC50s of from 5 to 33 nM.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 223104-29-8
- Formula: C21H19F2NO3
- Molecular Weight:371.38
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Membrane Transporter/Ion Channel Compound Library
HY-L011
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Publications Citing Use of MedChemExpress (MCE) SEA0400
More- Autophagy. 2021 Nov;17(11):3592-3606. [Abstract]
- Nat Commun. 2026 Jan 8;17(1):1253. [Abstract]
- J Clin Invest. 2016 Jan;126(1):112-22. [Abstract]
- Basic Res Cardiol. 2024 Oct;119(5):751-772. [Abstract]
- EMBO J. 2024 Jan;43(1):14-31. [Abstract]
- JACC Basic Transl Sci. 2016 Jun;1(4):251-266. [Abstract]
- JCI Insight. 2019 Apr 25;5(11):e128765. [Abstract]
- Cells. 2019 Dec 31;9(1):102. [Abstract]
- Cell Calcium. 2016 Jul;60(1):25-31. [Abstract]
- Cell Signal. 2017 Sep:37:12-30. [Abstract]
- J Physiol. 2015 Aug 1;593(15):3333-50. [Abstract]
- J Physiol. 2015 Mar 15;593(6):1459-77. [Abstract]
- J Physiol. 2014 Nov 15;592(Pt 22):4969-93. [Abstract]
- Am J Physiol Heart Circ Physiol. 2016 Dec 1;311(6):H1352-H1359. [Abstract]
- Nanotoxicology. 2026 May 19:1-18. [Abstract]
- Biochem Biophys Rep. 2017 Jan 11:9:245-256. [Abstract]
- Brain Res. 2016 Aug 1:1644:249-57. [Abstract]
- Pflugers Arch. 2015 Oct;467(10):2151-63. [Abstract]
- Biol Pharm Bull. 2023;46(8):1120-1127. [Abstract]
- bioRxiv. 2025 Jun 19:2025.06.17.660213. [Abstract]
Customer Validation & Images
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WB
Biological Activity
IC50: 5-33 nM (NCX)
SEA0400 inhibits Na+-dependent 45Ca2+ uptake in cultured neurons, astrocytes, and microglia. IC50 values of SEA0400 are 33 nM (neurons), 5.0 nM (astrocytes), and 8.3 nM (microglia)[1]. SEA0400 prevents sodium nitroprusside (SNP) to increase ERK and p38 MAPK phosphorylation, and production of reactive oxygen species (ROS) in an extracellular Ca2+-dependent manner[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 223104-29-8
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Appearance Solid
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Molecular Weight 371.38
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Formula C21H19F2NO3
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Color White to khaki
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SMILES
NC1=CC(OCC)=CC=C1OC2=CC=C(OCC3=CC(F)=CC=C3F)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (20)
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Journal Impact Factor
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Most Recent
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Autophagy
Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4. [Abstract]2021 Nov;17(11):3592-3606. PMID: 33629929 -
Nat Commun
2026 Jan 8;17(1):1253. PMID: 41507162 -
J Clin Invest
2016 Jan;126(1):112-22. PMID: 26595809 -
Basic Res Cardiol
Empagliflozin prevents heart failure through inhibition of the NHE1-NO pathway, independent of SGLT2. [Abstract]2024 Oct;119(5):751-772. PMID: 39046464 -
EMBO J
Structural insight into the allosteric inhibition of human sodium-calcium exchanger NCX1 by XIP and SEA0400. [Abstract]2024 Jan;43(1):14-31. PMID: 38177313 -
JACC Basic Transl Sci
Neuronal Na+ Channels Are Integral Components of Pro-arrhythmic Na+/Ca2+ Signaling Nanodomain That Promotes Cardiac Arrhythmias During β-adrenergic Stimulation. [Abstract]2016 Jun;1(4):251-266. PMID: 27747307 -
JCI Insight
Heterogeneity of the action potential duration is required for sustained atrial fibrillation. [Abstract]2019 Apr 25;5(11):e128765. PMID: 31021331 -
Cells
2019 Dec 31;9(1):102. PMID: 31906100 -
Cell Calcium
Paclitaxel-induced increase in NCX activity in subpopulations of nociceptive afferents: A protective mechanism against chemotherapy-induced peripheral neuropathy?. [Abstract]2016 Jul;60(1):25-31. PMID: 27166151 -
Cell Signal
Coupling between the TRPC3 ion channel and the NCX1 transporter contributed to VEGF-induced ERK1/2 activation and angiogenesis in human primary endothelial cells. [Abstract]2017 Sep:37:12-30. PMID: 28535874
SEA0400 purchased from MedChemExpress. Usage Cited in: Cell Signal. 2017 Sep:37:12-30. [Abstract]
HUVECs are incubated with the indicated concentrations of a second reverse-mode NCX inhibitor, SEA0400, for 30 min prior to stimulation with OAG (100 μM) for 5 min. ERK1/2 activation is assessed by Western blot.
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J Physiol
The role of Ca(2+) influx in spontaneous Ca(2+) wave propagation in interstitial cells of Cajal from the rabbit urethra. [Abstract]2015 Aug 1;593(15):3333-50. PMID: 26046824 -
J Physiol
Inositol-1,4,5-trisphosphate induced Ca2+ release and excitation-contraction coupling in atrial myocytes from normal and failing hearts. [Abstract]2015 Mar 15;593(6):1459-77. PMID: 25416623 -
J Physiol
The properties, distribution and function of Na(+)-Ca(2+) exchanger isoforms in rat cutaneous sensory neurons. [Abstract]2014 Nov 15;592(Pt 22):4969-93. PMID: 25239455 -
Am J Physiol Heart Circ Physiol
2016 Dec 1;311(6):H1352-H1359. PMID: 27694214 -
Nanotoxicology
Polystyrene nanoplastics promote tumor lung metastasis by inducing sodium overload in macrophages in an NMDAR-dependent way. [Abstract]2026 May 19:1-18. PMID: 42154607 -
Biochem Biophys Rep
Flecainide ameliorates arrhythmogenicity through NCX flux in Andersen-Tawil syndrome-iPS cell-derived cardiomyocytes. [Abstract]2017 Jan 11:9:245-256. PMID: 28956012 -
Brain Res
Na(+), K(+)-ATPase dysfunction causes cerebrovascular endothelial cell degeneration in rat prefrontal cortex slice cultures. [Abstract]2016 Aug 1:1644:249-57. PMID: 27208492 -
Pflugers Arch
Echinochrome A regulates phosphorylation of phospholamban Ser16 and Thr17 suppressing cardiac SERCA2A Ca²⁺ reuptake. [Abstract]2015 Oct;467(10):2151-63. PMID: 25410495 -
Biol Pharm Bull
The Antiarrhythmic Action of the Na+/Ca2+ Exchanger Inhibitor SEA0400 on Drug-Induced Long QT Syndrome Depends on the Severity of Proarrhythmic Conditions in Anesthetized Atrioventricular Block Rabbits. [Abstract]2023;46(8):1120-1127. PMID: 37532563 -
bioRxiv
2025 Jun 19:2025.06.17.660213. PMID: 40611907
Solvent & Solubility
DMSO : ≥ 32 mg/mL (86.17 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.73 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Na+-Ca2+ exchange activity is determined by assaying Na+-dependent 45Ca2+ uptake as reported previously. Briefly, the cells are preincubated in Hanks' balanced saline solution (HBSS) for 20 min, and the medium is switched to HBSS containing 45Ca2+ and incubated for 5 min. To increase intracellular Na+ concentration, 1 mM ouabain plus 20 μM monensin (for astrocytes and microglia) and 10 μM monensin (for neurons) are used. Monensin is added simultaneously with the isotope. Ouabain is added 5 min before monensin in astrocytes and microglia. SEA0400 and KB-R7943 are added 5 min before monensin and present during 45Ca2+ uptake reaction.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Cells, plated in 96-well plastic tissue culture plates, are incubated at 37°C for 30 min in normal or Ca2+-free HBSS containing 10 μM H2DCF-DA and 0.25 μg/mL Cremophor EL, and then rinsed twice with normal HBSS to remove excess dye. The cells are reperfused in normal HBSS for 1 h, and the conversion of H2DCF-DA to its fluorescent product dichlorofluorescein by ROS, presumably H2O2 and hydroxyl radical, is determined with excitation at 485 nm and emission at 535 nm using a Wallac Multilabel counter. ROS production is expressed as a percentage of control cells. The linearity and sensitivity of ROS assay are confirmed using H2O2 prior to the experiment. SEA0400 at the indicated concentrations is added 10 min before Ca2+ reperfusion and present until assay.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Male Sprague-Dawley rats, weighing 289 to 325 g, are anesthetized with 1 to 2% halothane. A catheter is inserted into the femoral artery and connected to a pressure transducer to record blood pressure. Regional cortical blood flow is measured by a laser Doppler flowmeter, with probe placement at 2 mm posterior and 6 mm lateral to the bregma. SEA0400 or its vehicle with an equivalent volume is i.v. injected at 3 mg/kg and then infused at 3 mg/kg/h for 2 h under normal conditions without MCA occlusion.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Matsuda T, et al. SEA0400, a novel and selective inhibitor of the Na+-Ca2+ exchanger, attenuates reperfusion injury in the in vitro and in vivo cerebral ischemic models. J Pharmacol Exp Ther. 2001 Jul;298(1):249-56. [Content Brief]
[2]. Nashida T, et al. The specific Na(+)/Ca(2+) exchange inhibitor SEA0400 prevents nitric oxide-induced cytotoxicity in SH-SY5Y cells. Neurochem Int. 2011 Aug;59(1):51-8. [Content Brief]
[3]. Ago Y, et al. SEA0400, a specific Na+/Ca2+ exchange inhibitor, prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease. Neuropharmacology. 2011 Dec;61(8):1441-51. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6927 mL | 13.4633 mL | 26.9266 mL | 67.3165 mL |
| 5 mM | 0.5385 mL | 2.6927 mL | 5.3853 mL | 13.4633 mL | |
| 10 mM | 0.2693 mL | 1.3463 mL | 2.6927 mL | 6.7316 mL | |
| 15 mM | 0.1795 mL | 0.8976 mL | 1.7951 mL | 4.4878 mL | |
| 20 mM | 0.1346 mL | 0.6732 mL | 1.3463 mL | 3.3658 mL | |
| 25 mM | 0.1077 mL | 0.5385 mL | 1.0771 mL | 2.6927 mL | |
| 30 mM | 0.0898 mL | 0.4488 mL | 0.8976 mL | 2.2439 mL | |
| 40 mM | 0.0673 mL | 0.3366 mL | 0.6732 mL | 1.6829 mL | |
| 50 mM | 0.0539 mL | 0.2693 mL | 0.5385 mL | 1.3463 mL | |
| 60 mM | 0.0449 mL | 0.2244 mL | 0.4488 mL | 1.1219 mL | |
| 80 mM | 0.0337 mL | 0.1683 mL | 0.3366 mL | 0.8415 mL |
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