Cat. No.: HY-18728

STF-31

17 Cited Publications

Customer Review

Based on 17 publication(s) in Google Scholar

STF-31 is a selective inhibitor of glucose transporter 1 (GLUT1), with an IC₅₀ of 1 μM. STF-31 is also a NAMPT inhibitor. STF-31 inhibits glucose uptake in renal cell carcinoma (RCC) 4 cells.

For research use only. We do not sell to patients.

Purity: 99.79%
CAS No.: 724741-75-7
Formula: C₂₃H₂₅N₃O₃S
Molecular Weight:423.53

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

STF-31 Chemical Structure

CAS No.: 724741-75-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (17)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]

GLUT1

1 μM (IC₅₀)

All GLUT Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.12 μM Compound: 51	Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay	[PMID: 24433859]
A2780	IC₅₀	120 nM Compound: 76; STF-31	Antiproliferative activity against human A2780 cells Antiproliferative activity against human A2780 cells	[PMID: 38244254]
A2780	IC₅₀	24 nM Compound: 51	Inhibition of NAMPT in human A2780 cells assessed as reduction in NAD level after 48 hrs by LC-MS/MS analysis Inhibition of NAMPT in human A2780 cells assessed as reduction in NAD level after 48 hrs by LC-MS/MS analysis	[PMID: 24433859]
HCT-116	IC₅₀	271 nM Compound: 51	Antiproliferative activity against human HCT116 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human HCT116 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
HT-1080	IC₅₀	213 nM Compound: 51	Antiproliferative activity against human HT1080 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human HT1080 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
MIA PaCa-2	IC₅₀	855 nM Compound: 51	Antiproliferative activity against human MIAPaCa2 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human MIAPaCa2 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
PC-3	IC₅₀	217 nM Compound: 51	Antiproliferative activity against human PC3 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human PC3 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
RCC4	IC₅₀	0.16 μM Compound: 2	Cytotoxicity against human RCC4 cells after 4 days by XTT assay Cytotoxicity against human RCC4 cells after 4 days by XTT assay	[PMID: 24387866]
RCC4	IC₅₀	7.9 μM Compound: 33; STF-31	Growth inhibition of VHL-deficient human RCC4 cells by XTT assay Growth inhibition of VHL-deficient human RCC4 cells by XTT assay	[PMID: 28042452]
RCC4/VHL	IC₅₀	>40 μM Compound: 2	Cytotoxicity against human RCC4/VHL cells after 4 days by XTT assay Cytotoxicity against human RCC4/VHL cells after 4 days by XTT assay	[PMID: 24387866]

In Vitro

STF-31 (0.01-10 μM; 10 days) is specifically toxic to RCC4 cells, whereas RCC4/VHL cells are relatively unaffected. RCC4/VHL cells treated with STF-31 (5 μM; 10 days) largely recovery, whereas RCC4 cells under the same conditions does not^[1].
STF-31 (1.25-5 μM; 3 days) does not induce autophagy, apoptosis, or DNA damage. STF-31 causes a necrotic cell death^[1].
STF-31 reduces the efferocytosis of wild type and SLC2A1-overexpressing LR73 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Wild-type RCC4 cells (RCC4/VHL) and RCC4 cells without VHL
Concentration:

0.01, 0.1, 1, 10 μM
Incubation Time:

10 days
Result:

Reduced viability of RCC4 cells without VHL in a concentration-dependent manner when compared to their wild-type counterparts.

In Vivo

STF-31 (10 mg/kg; i.p.; twice daily for 2 days, followed by once daily for another 3 days) does not affect normal mice body weight, behavior, and ERG responses. STF-31 reduces light-induced CX3CR1^gfp/+ mice microglial activation and retinal degeneration^[3].
STF-31 (10 mg/kg; i.p.) promotes accumulation of necrotic thymocytes after Dexamethasone-induced apoptosis in vivo^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Twelve-week old C57BL/6J and CX3CR1^gfp/+ mice^[3]
Dosage:

10 mg/kg
Administration:

I.p. injections; twice daily for 2 days, followed by once daily for another 3 days
Result:

Improved photoreceptor survival and reduced microglial activation of CX3CR1^gfp/+ mice, and not induced any C57BL/6J mice retinal cell death.

Chemical Information

CAS No. 724741-75-7
Appearance Solid
Molecular Weight 423.53
Formula C₂₃H₂₅N₃O₃S
Color Light yellow to yellow
SMILES

O=C(NC1=CC=CN=C1)C2=CC=C(CNS(=O)(C3=CC=C(C(C)(C)C)C=C3)=O)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (17)

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Journal Impact Factor

Cancer Cell

Hypoxia inducible factor-1α drives cancer resistance to cuproptosis. [Abstract]

2025 May 12;43(5):937-954.e9. PMID: 40054467
Chem Eng J

2024 Nov 15.
Cell Rep

Metabolic reinvigoration of NK cells by IL-21 enhances immunotherapy against MHC class I-deficient solid tumors. [Abstract]

2026 Feb 25;45(3):117035. PMID: 41758652
Arch Toxicol

CuO-NPs-triggered heterophil extracellular traps exacerbate liver injury in chicks by promoting oxidative stress and inflammatory responses. [Abstract]

2022 Nov;96(11):2913-2926. PMID: 35962800
Arch Toxicol

Chicken heterophils extracellular traps act as early effectors against cyclopiazonic acid dependent upon NADPH oxidase, ROS and glycolysis. [Abstract]

2022 Jul;96(7):2113-2122. PMID: 35508807
Cell Biosci

GLUT1-mediated microglial proinflammatory activation contributes to the development of stress-induced spatial learning and memory dysfunction in mice. [Abstract]

2024 Apr 16;14(1):48. PMID: 38627830

STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]

qRT-PCR assays evaluating the expression of proinflammatory phenotype markers, CD86, IL-6, IL-1β and TNF-α in BV2 cells exposed to GLUT1 inhibitors (STF-31, 5 µmol/L and BAY-876, 50 nmol/L) for 48 h with or without glucose treatment.

STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]

Flow cytometry analysis of CD86⁺ populations in BV2 cells exposed to STF-31 (5 μM) or BAY-876 under control or glucose conditions. Representative images.

STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]

Levels of IκBα in BV2 cells exposed to STF-31 (5 μM) or BAY-876 upon glucose treatment were determined by western blotting.

STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]

NF-κB nuclear translocation in BV2 cells exposed to STF-31 (5 μM) or BAY-876 under glucose incubation was analyzed by IF staining.
J Agric Food Chem

Glycolysis and Reactive Oxygen Species Production Participate in T-2 Toxin-Stimulated Chicken Heterophil Extracellular Traps. [Abstract]

2021 Nov 3;69(43):12862-12869. PMID: 34694797
Molecules

The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells. [Abstract]

2023 Jun 6;28(12):4569. PMID: 37375124
Poult Sci

Gliotoxin elicits immunotoxicity in the early innate immune system of ducks. [Abstract]

2024 Jun;103(6):103717. PMID: 38643746
Fish Shellfish Immunol

Zinc oxide nanoparticles (ZnO-NPs) exhibit immune toxicity to crucian carp (Carassius carassius) by neutrophil extracellular traps (NETs) release and oxidative stress. [Abstract]

2022 Oct:129:22-29. PMID: 35932984
Exp Cell Res

p38 MAPK-mediated suppression of Nrf2-MPC2 axis drives metabolic reprogramming which confers imatinib resistance in blast crisis phase of chronic myeloid leukemia. [Abstract]

2025 Dec 19;455(2):114870. PMID: 41423144
Food Chem Toxicol

Diacetoxyscirpenol-induced heterophil extracellular traps contribute to the immune toxicity of liver injury in chickens. [Abstract]

2021 Feb:148:111926. PMID: 33352262
J Inorg Biochem

Nanosilver-stimulated heterophil extracellular traps promoted liver and kidney injury in chicken. [Abstract]

2022 Apr 20;233:111838. PMID: 35504082
J Inorg Biochem

Alumina nanoparticles-induced heterophil extracellular traps exacerbate liver injury by regulating oxidative stress and inflammation in chickens. [Abstract]

2022 Apr:229:111725. PMID: 35063926
Vet Parasitol

Neospora caninum triggers the release of heterophil extracellular traps in chickens. [Abstract]

2025 Apr 15:337:110471. PMID: 40245810
SSRN

2024 Apr 8.
Research Square Preprint

2023 Jul 6.

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (80.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3611 mL

11.8055 mL

23.6111 mL

5 mM

0.4722 mL

2.3611 mL

4.7222 mL

10 mM

0.2361 mL

1.1806 mL

2.3611 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (5.90 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chan DA, et al. Targeting GLUT1 and the Warburg effect in renal cell carcinoma by chemical synthetic lethality. Sci Transl Med. 2011 Aug 3;3(94):94ra70. [Content Brief]

[2]. Dedda CD, et al. Pharmacological Targeting of GLUT1 to Control Autoreactive T Cell Responses. International Journal of Molecular Sciences. 2019 Oct 8; 20(19):4962. [Content Brief]

[3]. Wang L, et al. Glucose transporter 1 critically controls microglial activation through facilitating glycolysis. Molecular Neurodegeneration, 2019 Jan 11; 14(1):2. [Content Brief]

[4]. Morioka S, et, al. Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Nature. 2018 Nov;563(7733):714-718. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3611 mL	11.8055 mL	23.6111 mL	59.0277 mL
	5 mM	0.4722 mL	2.3611 mL	4.7222 mL	11.8055 mL
	10 mM	0.2361 mL	1.1806 mL	2.3611 mL	5.9028 mL
	15 mM	0.1574 mL	0.7870 mL	1.5741 mL	3.9352 mL
	20 mM	0.1181 mL	0.5903 mL	1.1806 mL	2.9514 mL
	25 mM	0.0944 mL	0.4722 mL	0.9444 mL	2.3611 mL
	30 mM	0.0787 mL	0.3935 mL	0.7870 mL	1.9676 mL
	40 mM	0.0590 mL	0.2951 mL	0.5903 mL	1.4757 mL
	50 mM	0.0472 mL	0.2361 mL	0.4722 mL	1.1806 mL
	60 mM	0.0394 mL	0.1968 mL	0.3935 mL	0.9838 mL
	80 mM	0.0295 mL	0.1476 mL	0.2951 mL	0.7378 mL

Keywords

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STF-31

Membrane Transporter/Ion Channel Compound Library

Autophagy Compound Library

Glycolysis Compound Library

Glutamine Metabolism Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Targeted Diversity Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) STF-31

All GLUT Isoforms

Biological Activity

Chemical Information

Publications (17)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

STF-31

Membrane Transporter/Ion Channel Compound Library

Autophagy Compound Library

Glycolysis Compound Library

Glutamine Metabolism Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Targeted Diversity Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) STF-31

All GLUT Isoforms

Biological Activity

Chemical Information

Publications (17)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

STF-31 Related Classifications

HY-18728 Related Classifications