1. Apoptosis Protein Tyrosine Kinase/RTK JAK/STAT Signaling Epigenetics Stem Cell/Wnt
  2. Apoptosis JAK
  3. JAK-2/3-IN-3

JAK-2-/3-IN-3 (compound ST4j) is a potent JAK2/3 inhibitor with IC50s of 13.00 and 14.86 nM for JAK2 and JAK3, respectively. JAK-2-/3-IN-3 inhibits autophosphorylation of JAK2 and induces apoptosis in a dose- and time-dependent manner. JAK-2-/3-IN-3 can be used in studies of lymph derived diseases and leukemia.

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JAK-2/3-IN-3 Chemical Structure

JAK-2/3-IN-3 Chemical Structure

CAS No. : 2242031-31-6

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Description

JAK-2-/3-IN-3 (compound ST4j) is a potent JAK2/3 inhibitor with IC50s of 13.00 and 14.86 nM for JAK2 and JAK3, respectively. JAK-2-/3-IN-3 inhibits autophosphorylation of JAK2 and induces apoptosis in a dose- and time-dependent manner. JAK-2-/3-IN-3 can be used in studies of lymph derived diseases and leukemia[1].

IC50 & Target[1]

JAK2

13.00 nM (IC50)

JAK3

14.86 nM (IC50)

In Vitro

JAK-2/3-IN-3 (0-100 μM; 72 h) specifically inhibits human erythroleukemia cells with low toxicity to the normal cells[1].
JAK-2/3-IN-3 inhibits human erythroleukemia TF1 cell growth via the JAK2/STAT5 signaling pathway[1].
JAK-2/3-IN-3 (15.53 μM; 24, 48, 72 h) induces cytotoxicity via apoptosis in a time-dependent manner in TF1 cells[1].
JAK-2/3-IN-3 (IC25, IC50, IC75; 24 h) induces cytotoxicity via apoptosis in a dose-dependent manner in TF1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: TF1 cells
Concentration: 15.53 μM
Incubation Time: 24, 48, 72 h
Result: Increased cells apoptotic by ∼20%, ∼40% and ∼70%, after 24-, 48- and 72-h incubations, respectively.
Increased cells apoptotic by ∼15%, 20%, and 30% with concentration of IC25, IC50 and IC75, respectively.

Cell Cytotoxicity Assay[1]

Cell Line: TF1, HEL, Vero and HepG2 cells
Concentration: 0-100 μM
Incubation Time: 72 h
Result: Inhibited growth of TF1 and HEL cells, with IC50s of 15.53 and 17.90 μM.
Showed low toxic to normal Vero cells and HepG2 cells (IC50 of >100 μM in Vero cells and of >50 μM in HepG2 cells).
Molecular Weight

325.15

Formula

C13H10Cl2N4O2

CAS No.
SMILES

O=C1NC2=C(N=C1C3=C(N(C)N=C3C)O)C=C(Cl)C(Cl)=C2

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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JAK-2/3-IN-3
Cat. No.:
HY-151285
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