Cat. No.: HY-151285

JAK-2/3-IN-3

Customer Review

Based on 1 Customer Validation

JAK-2-/3-IN-3 (compound ST4j) is a potent JAK2/3 inhibitor with IC₅₀s of 13.00 and 14.86 nM for JAK2 and JAK3, respectively. JAK-2-/3-IN-3 inhibits autophosphorylation of JAK2 and induces apoptosis in a dose- and time-dependent manner. JAK-2-/3-IN-3 can be used in studies of lymph derived diseases and leukemia.

For research use only. We do not sell to patients.

CAS No.: 2242031-31-6
Formula: C₁₃H₁₀Cl₂N₄O₂
Molecular Weight:325.15

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Data Sheet (276 KB)

JAK-2/3-IN-3 Chemical Structure

CAS No.: 2242031-31-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

JAK2

13.00 nM (IC₅₀)

JAK3

14.86 nM (IC₅₀)

All JAK Isoforms :

In Vitro

JAK-2/3-IN-3 (0-100 μM; 72 h) specifically inhibits human erythroleukemia cells with low toxicity to the normal cells^[1].
JAK-2/3-IN-3 inhibits human erythroleukemia TF1 cell growth via the JAK2/STAT5 signaling pathway^[1].
JAK-2/3-IN-3 (15.53 μM; 24, 48, 72 h) induces cytotoxicity via apoptosis in a time-dependent manner in TF1 cells^[1].
JAK-2/3-IN-3 (IC₂₅, IC₅₀, IC₇₅; 24 h) induces cytotoxicity via apoptosis in a dose-dependent manner in TF1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

TF1 cells
Concentration:

15.53 μM
Incubation Time:

24, 48, 72 h
Result:

Increased cells apoptotic by ∼20%, ∼40% and ∼70%, after 24-, 48- and 72-h incubations, respectively.
Increased cells apoptotic by ∼15%, 20%, and 30% with concentration of IC₂₅, IC₅₀ and IC₇₅, respectively.

Cell Cytotoxicity Assay^[1]

Cell Line:

TF1, HEL, Vero and HepG2 cells
Concentration:

0-100 μM
Incubation Time:

72 h
Result:

Inhibited growth of TF1 and HEL cells, with IC₅₀s of 15.53 and 17.90 μM.
Showed low toxic to normal Vero cells and HepG2 cells (IC₅₀ of >100 μM in Vero cells and of >50 μM in HepG2 cells).

Chemical Information

CAS No. 2242031-31-6
Appearance Solid
Molecular Weight 325.15
Formula C₁₃H₁₀Cl₂N₄O₂
Color Light yellow to yellow
SMILES

O=C1NC2=C(N=C1C3=C(N(C)N=C3C)O)C=C(Cl)C(Cl)=C2
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 87.5 mg/mL (269.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0755 mL

15.3775 mL

30.7550 mL

5 mM

0.6151 mL

3.0755 mL

6.1510 mL

10 mM

0.3076 mL

1.5378 mL

3.0755 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sanachai K, et al. Discovery of JAK2/3 Inhibitors from Quinoxalinone-Containing Compounds. ACS Omega, 2022.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0755 mL	15.3775 mL	30.7550 mL	76.8876 mL
	5 mM	0.6151 mL	3.0755 mL	6.1510 mL	15.3775 mL
	10 mM	0.3076 mL	1.5378 mL	3.0755 mL	7.6888 mL
	15 mM	0.2050 mL	1.0252 mL	2.0503 mL	5.1258 mL
	20 mM	0.1538 mL	0.7689 mL	1.5378 mL	3.8444 mL
	25 mM	0.1230 mL	0.6151 mL	1.2302 mL	3.0755 mL
	30 mM	0.1025 mL	0.5126 mL	1.0252 mL	2.5629 mL
	40 mM	0.0769 mL	0.3844 mL	0.7689 mL	1.9222 mL
	50 mM	0.0615 mL	0.3076 mL	0.6151 mL	1.5378 mL
	60 mM	0.0513 mL	0.2563 mL	0.5126 mL	1.2815 mL
	80 mM	0.0384 mL	0.1922 mL	0.3844 mL	0.9611 mL
	100 mM	0.0308 mL	0.1538 mL	0.3076 mL	0.7689 mL

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JAK-2/3-IN-3

Apoptosis Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Anti-Liver Cancer Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Autoimmune Disease Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

All JAK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

JAK-2/3-IN-3 Related Classifications

HY-151285 Related Classifications