2. Apoptosis Protein Tyrosine Kinase/RTK JAK/STAT Signaling Epigenetics Stem Cell/Wnt
  3. Apoptosis JAK
  4. JAK-2/3-IN-3

JAK-2-/3-IN-3 (compound ST4j) is a potent JAK2/3 inhibitor with IC50s of 13.00 and 14.86 nM for JAK2 and JAK3, respectively. JAK-2-/3-IN-3 inhibits autophosphorylation of JAK2 and induces apoptosis in a dose- and time-dependent manner. JAK-2-/3-IN-3 can be used in studies of lymph derived diseases and leukemia.

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JAK-2/3-IN-3

JAK-2/3-IN-3 Chemical Structure

CAS No. : 2242031-31-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

JAK-2/3-IN-3 Related Antibodies

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Description

JAK-2-/3-IN-3 (compound ST4j) is a potent JAK2/3 inhibitor with IC50s of 13.00 and 14.86 nM for JAK2 and JAK3, respectively. JAK-2-/3-IN-3 inhibits autophosphorylation of JAK2 and induces apoptosis in a dose- and time-dependent manner. JAK-2-/3-IN-3 can be used in studies of lymph derived diseases and leukemia[1].

IC50 & Target[1]

JAK2

13.00 nM (IC50)

JAK3

14.86 nM (IC50)

In Vitro

JAK-2/3-IN-3 (0-100 μM; 72 h) specifically inhibits human erythroleukemia cells with low toxicity to the normal cells[1].
JAK-2/3-IN-3 inhibits human erythroleukemia TF1 cell growth via the JAK2/STAT5 signaling pathway[1].
JAK-2/3-IN-3 (15.53 μM; 24, 48, 72 h) induces cytotoxicity via apoptosis in a time-dependent manner in TF1 cells[1].
JAK-2/3-IN-3 (IC25, IC50, IC75; 24 h) induces cytotoxicity via apoptosis in a dose-dependent manner in TF1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JAK-2/3-IN-3 Related Antibodies

Apoptosis Analysis[1]

Cell Line: TF1 cells
Concentration: 15.53 μM
Incubation Time: 24, 48, 72 h
Result: Increased cells apoptotic by ∼20%, ∼40% and ∼70%, after 24-, 48- and 72-h incubations, respectively.
Increased cells apoptotic by ∼15%, 20%, and 30% with concentration of IC25, IC50 and IC75, respectively.

Cell Cytotoxicity Assay[1]

Cell Line: TF1, HEL, Vero and HepG2 cells
Concentration: 0-100 μM
Incubation Time: 72 h
Result: Inhibited growth of TF1 and HEL cells, with IC50s of 15.53 and 17.90 μM.
Showed low toxic to normal Vero cells and HepG2 cells (IC50 of >100 μM in Vero cells and of >50 μM in HepG2 cells).
Molecular Weight

325.15

Formula

C13H10Cl2N4O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC2=C(N=C1C3=C(N(C)N=C3C)O)C=C(Cl)C(Cl)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 87.5 mg/mL (269.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0755 mL 15.3775 mL 30.7550 mL
5 mM 0.6151 mL 3.0755 mL 6.1510 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0755 mL 15.3775 mL 30.7550 mL 76.8876 mL
5 mM 0.6151 mL 3.0755 mL 6.1510 mL 15.3775 mL
10 mM 0.3076 mL 1.5378 mL 3.0755 mL 7.6888 mL
15 mM 0.2050 mL 1.0252 mL 2.0503 mL 5.1258 mL
20 mM 0.1538 mL 0.7689 mL 1.5378 mL 3.8444 mL
25 mM 0.1230 mL 0.6151 mL 1.2302 mL 3.0755 mL
30 mM 0.1025 mL 0.5126 mL 1.0252 mL 2.5629 mL
40 mM 0.0769 mL 0.3844 mL 0.7689 mL 1.9222 mL
50 mM 0.0615 mL 0.3076 mL 0.6151 mL 1.5378 mL
60 mM 0.0513 mL 0.2563 mL 0.5126 mL 1.2815 mL
80 mM 0.0384 mL 0.1922 mL 0.3844 mL 0.9611 mL
100 mM 0.0308 mL 0.1538 mL 0.3076 mL 0.7689 mL
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JAK-2/3-IN-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JAK-2/3-IN-32242031-31-6ApoptosisJAKJanus kinaseTF1HELVeroHepG2lymph derived diseasesleukemiacancerInhibitorinhibitorinhibit

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