2. Protein Tyrosine Kinase/RTK Apoptosis
  3. VEGFR Apoptosis
  4. Jolkinolide A

Jolkinolide A is a diterpenoid, can be extracted from the roots of Euphorbia fischeriana Steud. Jolkinolide A exhibits anti-tumor activity, by affecting on angiogenesis of tumor tissues. Jolkinolide A significantly inhibits the Akt-STAT3-mTOR signaling pathway and reduces the expression of VEGF in A549 cells.

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Jolkinolide A

Jolkinolide A Chemical Structure

CAS No. : 37905-07-0

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Jolkinolide A Related Antibodies

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Description

Jolkinolide A is a diterpenoid, can be extracted from the roots of Euphorbia fischeriana Steud. Jolkinolide A exhibits anti-tumor activity, by affecting on angiogenesis of tumor tissues. Jolkinolide A significantly inhibits the Akt-STAT3-mTOR signaling pathway and reduces the expression of VEGF in A549 cells[1].

IC50 & Target

VEGF[1]
Cellular Effect
Cell Line Type Value Description References
HCT-15 IC50
>50 μM
Compound: 6d
Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay
[PMID: 33289552]
LNCaP C4-2B IC50
10 μM
Compound: 6d
Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay
Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay
[PMID: 33289552]
MDA-MB-231 IC50
>50 μM
Compound: 6d
Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay
[PMID: 33289552]
RAW264.7 IC50
25 μM
Compound: 6
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
[PMID: 26615888]
In Vitro

Jolkinolide A (20-100 μg/mL; 24 h) increases the protein level of caspase-9 in A549 cells[1].
Jolkinolide A (40-80 μg/mL; 24 h) reduces the expression of Akt-STAT3-mTOR proteins in A549 cells[1].
Jolkinolide A (20-100 μg/mL; 24 h) inhibits human umbilical vein endothelial cells (HUVECs) proliferation and migration, and promotes apoptosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Jolkinolide A Related Antibodies

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 20 μg/mL, 40 μg/mL, 60 μg/mL, 80 μg/mL, and 100 μg/mL
Incubation Time: 24 hours
Result: Promoted caspase-9 protein expression in cells at 20-100 μg/mL. Inhibited expression levels of Akt, STAT3, and mTOR proteins in cells at 40-80 μg/mL.
In Vivo

Jolkinolide A (40 mg/kg; i.p.; once every 3 days, for 2 months) inhibits the expression of the VEGF protein in A549 cell xenograft tumor model in mouse[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c nude mice (4-5 weeks old) injected with A549 cells[1]
Dosage: 40 mg/kg
Administration: Intraperitoneal injection; once every 3 days for 2 months
Result: Significantly inhibited the expression of VEGF protein on the first, 10th, 30th, 45th, and 60th day of administration.
Molecular Weight

314.42

Formula

C20H26O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C2[C@H]3O[C@]3([C@H]4C=C2OC1=O)CC[C@H]5[C@]4(CCCC5(C)C)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 2 mg/mL (6.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1805 mL 15.9023 mL 31.8046 mL
5 mM 0.6361 mL 3.1805 mL 6.3609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1805 mL 15.9023 mL 31.8046 mL 79.5115 mL
5 mM 0.6361 mL 3.1805 mL 6.3609 mL 15.9023 mL
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Jolkinolide A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Jolkinolide A37905-07-0VEGFRApoptosisVascular endothelial growth factor receptorInhibitorinhibitorinhibit

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