Cat. No.: HY-119767

Jolkinolide A

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Jolkinolide A is a diterpenoid, can be extracted from the roots of Euphorbia fischeriana Steud. Jolkinolide A exhibits anti-tumor activity, by affecting on angiogenesis of tumor tissues. Jolkinolide A significantly inhibits the Akt-STAT3-mTOR signaling pathway and reduces the expression of VEGF in A549 cells.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 37905-07-0
Formula: C₂₀H₂₆O₃
Molecular Weight:314.42

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Jolkinolide A Chemical Structure

CAS No.: 37905-07-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

VEGF^[1]

All VEGFR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-15	IC₅₀	>50 μM Compound: 6d	Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay	[PMID: 33289552]
LNCaP C4-2B	IC₅₀	10 μM Compound: 6d	Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay	[PMID: 33289552]
MDA-MB-231	IC₅₀	>50 μM Compound: 6d	Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay	[PMID: 33289552]
RAW264.7	IC₅₀	25 μM Compound: 6	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 26615888]

In Vitro

Jolkinolide A (20-100 μg/mL; 24 h) increases the protein level of caspase-9 in A549 cells^[1].
Jolkinolide A (40-80 μg/mL; 24 h) reduces the expression of Akt-STAT3-mTOR proteins in A549 cells^[1].
Jolkinolide A (20-100 μg/mL; 24 h) inhibits human umbilical vein endothelial cells (HUVECs) proliferation and migration, and promotes apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

A549 cells
Concentration:

20 μg/mL, 40 μg/mL, 60 μg/mL, 80 μg/mL, and 100 μg/mL
Incubation Time:

24 hours
Result:

Promoted caspase-9 protein expression in cells at 20-100 μg/mL. Inhibited expression levels of Akt, STAT3, and mTOR proteins in cells at 40-80 μg/mL.

In Vivo

Jolkinolide A (40 mg/kg; i.p.; once every 3 days, for 2 months) inhibits the expression of the VEGF protein in A549 cell xenograft tumor model in mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female BALB/c nude mice (4-5 weeks old) injected with A549 cells^[1]
Dosage:

40 mg/kg
Administration:

Intraperitoneal injection; once every 3 days for 2 months
Result:

Significantly inhibited the expression of VEGF protein on the first, 10th, 30th, 45th, and 60th day of administration.

Chemical Information

CAS No. 37905-07-0
Appearance Solid
Molecular Weight 314.42
Formula C₂₀H₂₆O₃
Color White to off-white
SMILES

CC1=C2[C@H]3O[C@]3([C@H]4C=C2OC1=O)CC[C@H]5[C@]4(CCCC5(C)C)C
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 2 mg/mL (6.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1805 mL

15.9023 mL

31.8046 mL

5 mM

0.6361 mL

3.1805 mL

6.3609 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Shen L, et al. Jolkinolide A and Jolkinolide B Inhibit Proliferation of A549 Cells and Activity of Human Umbilical Vein Endothelial Cells. Med Sci Monit. 2017 Jan 14;23:223-237. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1805 mL	15.9023 mL	31.8046 mL	79.5115 mL
DMSO	5 mM	0.6361 mL	3.1805 mL	6.3609 mL	15.9023 mL

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Jolkinolide A

Apoptosis Compound Library

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All VEGFR Isoforms