2. Protein Tyrosine Kinase/RTK Apoptosis
  3. VEGFR Apoptosis
  4. Jolkinolide A

Jolkinolide A is a diterpenoid, can be extracted from the roots of Euphorbia fischeriana Steud. Jolkinolide A exhibits anti-tumor activity, by affecting on angiogenesis of tumor tissues. Jolkinolide A significantly inhibits the Akt-STAT3-mTOR signaling pathway and reduces the expression of VEGF in A549 cells.

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Jolkinolide A

Jolkinolide A Chemical Structure

CAS No. : 37905-07-0

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Based on 1 publication(s) in Google Scholar

Jolkinolide A Related Antibodies

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Description

Jolkinolide A is a diterpenoid, can be extracted from the roots of Euphorbia fischeriana Steud. Jolkinolide A exhibits anti-tumor activity, by affecting on angiogenesis of tumor tissues. Jolkinolide A significantly inhibits the Akt-STAT3-mTOR signaling pathway and reduces the expression of VEGF in A549 cells[1].

IC50 & Target

VEGF[1]
Cellular Effect
Cell Line Type Value Description References
HCT-15 IC50
> 50 μM
Compound: 6d
Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-15 cells incubated for 48 hrs by MTT assay
[PMID: 33289552]
LNCaP C4-2B IC50
10 μM
Compound: 6d
Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay
Cytotoxicity against human LNCaP C4-2B cells incubated for 48 hrs by MTT assay
[PMID: 33289552]
MDA-MB-231 IC50
> 50 μM
Compound: 6d
Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay
[PMID: 33289552]
RAW264.7 IC50
25 μM
Compound: 6
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
[PMID: 26615888]
In Vitro

Jolkinolide A (20-100 μg/mL; 24 h) increases the protein level of caspase-9 in A549 cells[1].
Jolkinolide A (40-80 μg/mL; 24 h) reduces the expression of Akt-STAT3-mTOR proteins in A549 cells[1].
Jolkinolide A (20-100 μg/mL; 24 h) inhibits human umbilical vein endothelial cells (HUVECs) proliferation and migration, and promotes apoptosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Jolkinolide A Related Antibodies

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 20 μg/mL, 40 μg/mL, 60 μg/mL, 80 μg/mL, and 100 μg/mL
Incubation Time: 24 hours
Result: Promoted caspase-9 protein expression in cells at 20-100 μg/mL. Inhibited expression levels of Akt, STAT3, and mTOR proteins in cells at 40-80 μg/mL.
In Vivo

Jolkinolide A (40 mg/kg; i.p.; once every 3 days, for 2 months) inhibits the expression of the VEGF protein in A549 cell xenograft tumor model in mouse[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c nude mice (4-5 weeks old) injected with A549 cells[1]
Dosage: 40 mg/kg
Administration: Intraperitoneal injection; once every 3 days for 2 months
Result: Significantly inhibited the expression of VEGF protein on the first, 10th, 30th, 45th, and 60th day of administration.
Molecular Weight

314.42

Formula

C20H26O3
CAS No.
Appearance

Solid

SMILES

CC1=C2[C@H]3O[C@]3([C@H]4C=C2OC1=O)CC[C@H]5[C@]4(CCCC5(C)C)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 2 mg/mL (6.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1805 mL 15.9023 mL 31.8046 mL
5 mM 0.6361 mL 3.1805 mL 6.3609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1805 mL 15.9023 mL 31.8046 mL 79.5115 mL
5 mM 0.6361 mL 3.1805 mL 6.3609 mL 15.9023 mL
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Jolkinolide A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Jolkinolide A37905-07-0VEGFRApoptosisVascular endothelial growth factor receptorInhibitorinhibitorinhibit

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