4-Hydroxyestradiol
Based on 1 publication(s) in Google Scholar
4-Hydroxyestradiol (4-Hydroxy-17β-estradiol) is an endogenous metabolite of Estradiol (HY-B0141). 4-Hydroxyestradiol is carcinogenic and shows mutagenic activity in breast epithelial cells. 4-Hydroxyestradiol inhibits the binding of Estradiol to the estrogen receptor in a competitive manner, with a Ki of 0.48 nM.
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 5976-61-4
- Formula: C18H24O3
- Molecular Weight:288.38
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Hydroxyestradiol
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | EC50 |
5.06 M
Compound: 4-hydroxy-estradiol
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Affinity for estrogen receptor of human MCF-7 cells
Affinity for estrogen receptor of human MCF-7 cells
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[PMID: 9371241] |
| MCF7 | EC50 |
5.06 x 10-7M
Compound: 4-hydroxy-estradiol
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Affinity for estrogen receptor of human MCF-7 cells
Affinity for estrogen receptor of human MCF-7 cells
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[PMID: 9371241] |
| MCF7 | EC50 |
6.54 M
Compound: 4-hydroxy-estradiol
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Induction of pS2 Gene expression in human MCF-7 cells
Induction of pS2 Gene expression in human MCF-7 cells
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[PMID: 9371241] |
| MCF7 | EC50 |
6.54 x 10-8M
Compound: 4-hydroxy-estradiol
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Induction of pS2 Gene expression in human MCF-7 cells
Induction of pS2 Gene expression in human MCF-7 cells
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[PMID: 9371241] |
Chemical Information
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CAS No. 5976-61-4
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Appearance Solid
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Molecular Weight 288.38
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Formula C18H24O3
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Color White to light yellow
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SMILES
C[C@@]12[C@](CC[C@@H]2O)([H])[C@@](CCC3=C4O)([H])[C@@](CC1)([H])C3=CC=C4O
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Synonyms
4-Hydroxy-17β-estradiol
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Sci Rep
2025 Mar 11;15(1):8409. PMID: 40069295
Solvent & Solubility
DMSO : 25 mg/mL (86.69 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
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Handling Instructions (2659 KB)
References
[1]. Fernandez SV, et al. Estradiol and its metabolites 4-hydroxyestradiol and 2-hydroxyestradiol induce mutations in human breast epithelial cells. Int J Cancer. 2006 Apr 15;118(8):1862-8. doi: 10.1002/ijc.21590. [Content Brief]
[2]. Van Aswegen CH, et al. Binding of 2-hydroxyestradiol and 4-hydroxyestradiol to estrogen receptors from human breast cancers. J Steroid Biochem. 1989 Apr;32(4):485-92. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4676 mL | 17.3382 mL | 34.6765 mL | 86.6912 mL |
| 5 mM | 0.6935 mL | 3.4676 mL | 6.9353 mL | 17.3382 mL | |
| 10 mM | 0.3468 mL | 1.7338 mL | 3.4676 mL | 8.6691 mL | |
| 15 mM | 0.2312 mL | 1.1559 mL | 2.3118 mL | 5.7794 mL | |
| 20 mM | 0.1734 mL | 0.8669 mL | 1.7338 mL | 4.3346 mL | |
| 25 mM | 0.1387 mL | 0.6935 mL | 1.3871 mL | 3.4676 mL | |
| 30 mM | 0.1156 mL | 0.5779 mL | 1.1559 mL | 2.8897 mL | |
| 40 mM | 0.0867 mL | 0.4335 mL | 0.8669 mL | 2.1673 mL | |
| 50 mM | 0.0694 mL | 0.3468 mL | 0.6935 mL | 1.7338 mL | |
| 60 mM | 0.0578 mL | 0.2890 mL | 0.5779 mL | 1.4449 mL | |
| 80 mM | 0.0433 mL | 0.2167 mL | 0.4335 mL | 1.0836 mL |
4-Hydroxyestradiol Related Classifications
HY-N10403 Related Classifications
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