BCA
Based on 8 publication(s) in Google Scholar
BCA is a chelating agent targeting CuI. BCA forms a water-soluble complex with CuI at a 2:1 stoichiometric ratio, which not only inhibits its oxidation and disproportionation reactions, but also mediates the CuI-catalyzed azide-alkyne cycloaddition click chemistry reaction to achieve bioconjugation of fluorophores or biotin with functionalized proteins. BCA chelates Cu+ from proteins that reduce Cu2+, forming a purple compound detectable by colorimetry, and thus is used to determine the total protein concentration in solution. BCA is a potential small-molecule candidate for AHAS inhibitors.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 979-88-4
- Formula: C20H10N2Na2O4
- Molecular Weight:388.29
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-D0020R
2,2'-Bipyridine (Standard)
Publications Citing Use of MedChemExpress (MCE) BCA
More- Adv Funct Mater. 2024 Mar 14.
- Cardiovasc Diabetol. 2019 Feb 2;18(1):15. [Abstract]
- Mater Today Bio. 2025 Feb 20:31:101598. [Abstract]
- Nano Today. 2025 Jun.
- Nano Today. 2025 Jun.
- Biologics. 2024 May 23:18:129-142. [Abstract]
- J Cancer. 2021 Feb 22;12(8):2243-2257. [Abstract]
- J Oncol. 2022 Aug 11;2022:5824617. [Abstract]
Biological Activity
BCA acts as a Cu (I) chelating stabilizer in CuI-catalyzed azide-alkyne cycloaddition reactions[1].
BCA (BCA) enables quantitative analysis of carbohydrates in three types of soil extracellular polymeric substance (EPS) extracts[2].
BCA (Compound 8) is a potential small-molecule candidate inhibitor of AHAS identified via virtual screening. It exhibits only 1.4 % inhibitory effect on rapeseed root growth at a concentration of 100 μg/mL, and shows no inhibitory activity at 10 μg/mL[3].
BCA (BCA) is a core reagent in commonly used BCA protein quantification methods. Under alkaline conditions, protein complexes with Cu2+ and is reduced to Cu+. BCA then binds with Cu+ to form a purple-blue complex. The absorbance of this complex is directly proportional to the protein concentration[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 979-88-4
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Appearance Solid
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Molecular Weight 388.29
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Formula C20H10N2Na2O4
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Color White to light yellow
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SMILES
O=C(O[Na])C1=CC(C2=NC3=C(C=CC=C3)C(C(O[Na])=O)=C2)=NC4=C1C=CC=C4
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Synonyms
Disodium bicinchoninate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (8)
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Journal Impact Factor
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Most Recent
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Cardiovasc Diabetol
SGLT2 inhibition with empagliflozin attenuates myocardial oxidative stress and fibrosis in diabetic mice heart. [Abstract]2019 Feb 2;18(1):15. PMID: 30710997 -
Mater Today Bio
A metal-polyphenol network-based iron supplement with improved stability and reduced gastrointestinal toxicity for iron deficiency anemia therapy. [Abstract]2025 Feb 20:31:101598. PMID: 40070867 -
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Biologics
LncRNA GAS5 Modulates the Progression of Glioma Through Repressing miR-135b-5p and Upregulating APC. [Abstract]2024 May 23:18:129-142. PMID: 38817552 -
J Cancer
MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma. [Abstract]2021 Feb 22;12(8):2243-2257. PMID: 33758602 -
J Oncol
Metformin Exhibits an Attractive Antineoplastic Effect on Human Endometrial Cancer by Regulating the Hippo Signaling Pathway. [Abstract]2022 Aug 11;2022:5824617. PMID: 36226249
Solvent & Solubility
H2O : 100 mg/mL (257.54 mM; Need ultrasonic)
7.5% Sodium bicarbonate : ≥ 50 mg/mL (128.77 mM)
DMSO : 2 mg/mL (5.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (64.38 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (290 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / 7.5% Sodium bicarbonate / H2O | 1 mM | 2.5754 mL | 12.8770 mL | 25.7539 mL | 64.3849 mL |
| 5 mM | 0.5151 mL | 2.5754 mL | 5.1508 mL | 12.8770 mL | |
| 7.5% Sodium bicarbonate / H2O | 10 mM | 0.2575 mL | 1.2877 mL | 2.5754 mL | 6.4385 mL |
| 15 mM | 0.1717 mL | 0.8585 mL | 1.7169 mL | 4.2923 mL | |
| 20 mM | 0.1288 mL | 0.6438 mL | 1.2877 mL | 3.2192 mL | |
| 25 mM | 0.1030 mL | 0.5151 mL | 1.0302 mL | 2.5754 mL | |
| 30 mM | 0.0858 mL | 0.4292 mL | 0.8585 mL | 2.1462 mL | |
| 40 mM | 0.0644 mL | 0.3219 mL | 0.6438 mL | 1.6096 mL | |
| 50 mM | 0.0515 mL | 0.2575 mL | 0.5151 mL | 1.2877 mL | |
| 60 mM | 0.0429 mL | 0.2146 mL | 0.4292 mL | 1.0731 mL | |
| 80 mM | 0.0322 mL | 0.1610 mL | 0.3219 mL | 0.8048 mL | |
| 100 mM | 0.0258 mL | 0.1288 mL | 0.2575 mL | 0.6438 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
BCA Related Classifications
HY-15908 Related Classifications
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