6-TAMRA
Based on 1 publication(s) in Google Scholar
6-TAMRA (6-Carboxytetramethylrhodamine) is a fluorescent dye. 6-TAMRA is often used in combination with 6-FAM (HY-66021) as an acceptor or fluorescence quencher for fluorescence resonance energy transfer (FRET) in biochemical studies such as potassium ion detection and ribonuclease activity assays.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.78%
- CAS 番号: 91809-67-5
- 分子式: C25H22N2O5
- 分子量:430.45
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
MedChemExpress(MCE)の使用を引用している文献 6-TAMRA
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生物活性
6-TAMRA, in the form of succinimidyl ester, undergoes conjugation with 3'-amino-modified oligonucleotides via incubation at 37℃ for 12 h in a pH 8.5 sodium bicarbonate buffer, and is further used as a 3'-end labeling dye for fluorescence quenching[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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化学情報
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CAS 番号 91809-67-5
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性状 Solid
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分子量 430.45
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分子式 C25H22N2O5
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Color Brown to black
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SMILES
CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC(C([O-])=O)=CC=C4C(O)=O)=C2C=C1)C
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別名
6-Carboxytetramethylrhodamine
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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J Control Release
Albumin binding revitalizes NQO1 bioactivatable drugs as novel therapeutics for pancreatic cancer. [Abstract]2022 Aug 4;349:876-889. PMID: 35907592
溶剤 & 溶解度
DMSO : 2.86 mg/mL (6.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Ueyama H, et al. A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation. J Am Chem Soc. 2002;124(48):14286-14287. [Content Brief]
[2]. Kelemen BR, et al. Hypersensitive substrate for ribonucleases. Nucleic Acids Res. 1999;27(18):3696-3701. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3232 mL | 11.6158 mL | 23.2315 mL | 58.0788 mL |
| 5 mM | 0.4646 mL | 2.3232 mL | 4.6463 mL | 11.6158 mL |