Luciferase
Based on 1 Customer Validation
Luciferase (bacterial) acts as a luminescence inducer and catalyst. Luciferase (bacterial) catalyzes the reduction of methylene blue, quinones, ferricyanide, and FMN using reduced DPN (HY-12452) (or an alternative electron donor for FMN). Luciferase (bacterial) binds to reduced FMN to form a luminescent reaction complex. Luciferase (bacterial) scavenges reactive oxygen species (ROS) and protects bacterial cells from oxidative stress damage. Luciferase (bacterial) is applicable to studies related to oxidative stress.
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- CAS 番号: 9014-00-0
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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HY-12591A
D-LuciferinSubstrate for Luciferase -
HY-12591
D-Luciferin sodiumSubstrate for Luciferase -
HY-P1004A
Luciferase, fireflyLight-Emitting Enzyme -
HY-B0075
MelatoninMelatonin Receptor Activator -
HY-12591B
D-Luciferin potassiumSubstrate for Luciferase -
HY-B2176
ATPEndogenous Metabolite -
HY-D0251
FluoresceinFluorescent Tracer -
HY-15922
LuminolChemiluminescent Substance -
HY-D0890
ABEIBiochemical Assay Reagent
生物活性
製品説明
体外実験
Luciferase (bacterial) catalyzes metal-independent oxidation of DPNH by ferricyanide, Methylene blue (HY-14536), and quinones in the absence of aldehyde and FMN, with ferricyanide competing with FMN for a reduced enzyme intermediate to suppress luminescence until fully reduced[1].
Luciferase (bacterial) exhibits species-specific differences in FMNH2 binding affinity and C4a-(hydro)peroxyflavin formation rates, with V. campbellii Lux displaying the highest FMNH2 binding affinity (Kd = 1.8 μM) and fastest oxygen reaction rate (253 s-1 at 0.26 mM oxygen)[3].
Luciferase (bacterial) (4-27 μM) binds aldehyde at two distinct sites, with high-affinity binding acting as a substrate interaction and lower-affinity binding at higher concentrations inhibiting catalytic activity by blocking FMNH2 binding and reducing C4a-(hydro)peroxyflavin formation[3].
Luciferase (bacterial) has specific active site and flexible loop residues critical for catalytic activity, intermediate stability, and light emission properties; mutations to these residues reduce bioluminescence efficiency, alter intermediate decay rates, or shift emission spectra[3].
Luciferase (bacterial) binds FMNH2 with an apparent Kd of 0.08 μM at room temperature, with initial bioluminescence intensity proportional to FMNH2 concentration and exponential first-order decay kinetics[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 9014-00-0
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性状 Solid
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Color White to light yellow
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SMILES
[Luciferase (bacterial)]
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Structure Classification
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Initial Source
Neonothopanus nambi fungus
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
溶剤 & 溶解度
体外:
H2O : 20 mg/mL (Need ultrasonic)
純度とドキュメンテーション
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データシート (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
[2]. Szpilewska H, et al. Experimental evidence for the physiological role of bacterial luciferase in the protection of cells against oxidative stress. Current microbiology. 2003 Nov;47(5):379-82. [Content Brief]
[3]. Tinikul R, et al. Bacterial luciferase: Molecular mechanisms and applications. The Enzymes. 2020;47:427-455. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Luciferase (bacterial) 関連分類
HY-P1004 関連分類
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