MS21570
Based on 1 Customer Validation
MS21570 is a selective GPR171 antagonist, with an IC50 of 220 nM.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.93%
- CAS 番号: 65373-29-7
- 分子式: C10H11N3S2
- 分子量:237.34
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
IC50: 220 nM (GPR171)[1].
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 65373-29-7
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性状 Solid
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分子量 237.34
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分子式 C10H11N3S2
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Color White to off-white
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SMILES
CNC1=NN=C(SCC2=CC=CC=C2)S1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
溶剤 & 溶解度
体外:
DMSO : 125 mg/mL (526.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
プロトコル
Animal Administration[1]
Mice[1]
Male C57bl/6J or AgRP-IRES-Cre mice are 8-12 weeks old at the beginning of the experiment. Mice are habituated to the testing room 1 h prior to each experiment. Behaviors are videotaped and analyzed. Mice are injected with MS21570 (MS0021570_1: 5 mg/kg, i.p.) 15 min prior to elevated plus maze, open field, forced swim and contextual fear conditioning. For microinjection studies, MS21570 is injected 10 min prior testing and immediately following the contextual fear conditioning. Singly housed AgRP-IRES-Cre mice with cre-inducible DREADD in the arcuate nucleus receive MS21570 (3.5 mg/kg, i.p.) or vehicle (6% DMSO in saline) 15 min prior to CNO (1 mg/kg, i.p.) or saline injection. Food intake is measured at 1, 2, 4, and 8 hours post-injection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
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データシート (273 KB)
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SDS (393 KB)
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- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.2134 mL | 21.0668 mL | 42.1336 mL | 105.3341 mL |
| 5 mM | 0.8427 mL | 4.2134 mL | 8.4267 mL | 21.0668 mL | |
| 10 mM | 0.4213 mL | 2.1067 mL | 4.2134 mL | 10.5334 mL | |
| 15 mM | 0.2809 mL | 1.4045 mL | 2.8089 mL | 7.0223 mL | |
| 20 mM | 0.2107 mL | 1.0533 mL | 2.1067 mL | 5.2667 mL | |
| 25 mM | 0.1685 mL | 0.8427 mL | 1.6853 mL | 4.2134 mL | |
| 30 mM | 0.1404 mL | 0.7022 mL | 1.4045 mL | 3.5111 mL | |
| 40 mM | 0.1053 mL | 0.5267 mL | 1.0533 mL | 2.6334 mL | |
| 50 mM | 0.0843 mL | 0.4213 mL | 0.8427 mL | 2.1067 mL | |
| 60 mM | 0.0702 mL | 0.3511 mL | 0.7022 mL | 1.7556 mL | |
| 80 mM | 0.0527 mL | 0.2633 mL | 0.5267 mL | 1.3167 mL | |
| 100 mM | 0.0421 mL | 0.2107 mL | 0.4213 mL | 1.0533 mL |
MS21570 関連分類
HY-112620 関連分類
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