Ac-LETD-AFC
Ac-LETD-AFC is a caspase-8 fluorogenic substrate. Ac-LETD-AFC can measure caspase-8 fluorogenic activity and can be used for the research of cancer cell apoptosis and oxidative stress metabolism.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 210345-02-1
- 分子式: C31H38F3N5O12
- 分子量:729.65
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Caspase activity assay[1]:
1. Incubate the cells according to your normal protocol.
2. Wash cells once with PBS and centrifugate.
3. Resuspend cells in PBS at a concentration of 1× 107 cells/mL.
4. Prepare the standard reaction buffer which includ 100 μM caspase-8 substrate AC-LETD-AFC. Prepare correspounding standard reaction buffer according to your protocol.
5. Add 15 ul of the cells suspension to a microplate, and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer.
6. Measure substrate cleavage with a microplate reader with excitation wavelength of 360 nm and emission at 460 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 210345-02-1
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分子量 729.65
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分子式 C31H38F3N5O12
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Ac-LETD-AFC 関連分類
HY-P2620 関連分類
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