Bis-Tris
Based on 1 Customer Validation
Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.0%
- CAS 番号: 6976-37-0
- 分子式: C8H19NO5
- 分子量:209.24
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保管条件:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Bis-Tris (10 mM or 4-12% Bis-Tris gel) has excellent buffering capacity and, in synergy with HEPES and cacodylic acid, covers a wide pH range. It can be used for fluorescence spectroscopy analysis, circular dichroism (CD) analysis, GST pull-down binding assay, gel filtration analysis, nuclear magnetic resonance (NMR) analysis, and protein electrophoresis. Abnormal pH fluctuations can affect experimental results[2][3].
Buffers containing Bis-Tris can be used to preserve purified proteins to prevent degradation (4°C)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 6976-37-0
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性状 Solid
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分子量 209.24
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分子式 C8H19NO5
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Color White to off-white
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SMILES
OCC(CO)(N(CCO)CCO)CO
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別名
ビス トリス
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
溶剤 & 溶解度
H2O : 200 mg/mL (955.84 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (238.96 mM); Clear solution; Need ultrasonic
純度とドキュメンテーション
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データシート (272 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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取扱説明書 (2659 KB)
参考文献
[2]. Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [Content Brief]
[3]. Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.7792 mL | 23.8960 mL | 47.7920 mL | 119.4800 mL |
| 5 mM | 0.9558 mL | 4.7792 mL | 9.5584 mL | 23.8960 mL | |
| 10 mM | 0.4779 mL | 2.3896 mL | 4.7792 mL | 11.9480 mL | |
| 15 mM | 0.3186 mL | 1.5931 mL | 3.1861 mL | 7.9653 mL | |
| 20 mM | 0.2390 mL | 1.1948 mL | 2.3896 mL | 5.9740 mL | |
| 25 mM | 0.1912 mL | 0.9558 mL | 1.9117 mL | 4.7792 mL | |
| 30 mM | 0.1593 mL | 0.7965 mL | 1.5931 mL | 3.9827 mL | |
| 40 mM | 0.1195 mL | 0.5974 mL | 1.1948 mL | 2.9870 mL | |
| 50 mM | 0.0956 mL | 0.4779 mL | 0.9558 mL | 2.3896 mL | |
| 60 mM | 0.0797 mL | 0.3983 mL | 0.7965 mL | 1.9913 mL | |
| 80 mM | 0.0597 mL | 0.2987 mL | 0.5974 mL | 1.4935 mL | |
| 100 mM | 0.0478 mL | 0.2390 mL | 0.4779 mL | 1.1948 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Bis-Tris 関連分類
HY-D0856 関連分類
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