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製品番号: HY-150048

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BK50164

BK50164 is a potent CD73 inhibitor with an IC₅₀ value of 13.089 μM. BK50164 binds to CD99 with a K_D value of 1.5 μM. BK50164 shows antiproliferative activity. BK50164 induced Apoptosis and cell cycle arrest at Sub-G1 phase.

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CAS 番号: 2204291-78-9
分子式: C₁₃H₁₃ClFN₅O₇
分子量:405.72

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

BK50164 構造式

CAS 番号: 2204291-78-9

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生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

IC₅₀: 13.089 µM (CD73)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
143B	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human 143B cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human 143B cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
A 172	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human A-172 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human A-172 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
A549	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
HEK293	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
HeLa	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
HOS	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human HOS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human HOS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
PC-3	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
RD	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human RD cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human RD cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
SAOS-2	IC₅₀	>=400 μM Compound: BK50164	Cytotoxicity against human SAOS-2 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human SAOS-2 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
SJRH30	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human Rh30 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human Rh30 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
TC-32	IC₅₀	27 μM Compound: BK50164	Cytotoxicity against human TC-32 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human TC-32 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
U2OS	IC₅₀	>=400 μM Compound: BK50164	Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
U-87MG ATCC	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human U-87 MG cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human U-87 MG cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]

体外実験

BK50164 (0-400 µM; 48 h) shows antiproliferative activity with IC₅₀s of 35.8, 34.28, 5.17, ≥400, ≥400 µM for A4573, TC-32, TC-71, Saos-2, U-2 OS cells, respectively^[2].
BK50164 (4-16 µM; 48 h) induces apoptosis and cell cycle arrest at Sub-G1 phase^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

A4573, TC-32, TC-71, Saos-2, U-2 OS cells
Concentration:

0-400 µM
Incubation Time:

48 h
Result:

Inhibited cell viability witn IC₅₀s of 35.8, 34.28, 5.17, ≥400, ≥400 µM for A4573, TC-32, TC-71, Saos-2, U-2 OS cells, respectively.

Cell Cycle Analysis^[2]

Cell Line:

TC-32, U-2 OS cells
Concentration:

4, 8, 16 µM
Incubation Time:

48 h
Result:

Induced apoptosis in only TC-32 ES cells but not in U-2 OS cell line and induces cell cycle arrest at Sub-G1 phase.

化学情報

CAS 番号 2204291-78-9
分子量 405.72
分子式 C₁₃H₁₃ClFN₅O₇
SMILES

NC1=NC(Cl)=NC2=C1N=CN2[C@@H]3O[C@H](COC(C(O)=O)C(O)=O)[C@@H](O)[C@@H]3F
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (274 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Roland Joseph Billedeau, et al. Ectonucleotidase inhibitors and methods of use thereof. WO2018119284A1.

[2]. Balaraman K, et al. Design, synthesis and biological evaluation of Nucleosidic CD99 inhibitors that selectively reduce Ewing sarcoma viability. Eur J Med Chem. 2023 May 5;251:115244. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

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濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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