CMB-087229
Based on 1 Customer Validation
CMB-087229 is a mutant superoxide dismutase 1 (SOD1) protein aggregation inhibitor with IC50 of 67 nM, which can be used in the research of amyotrophic lateral sclerosis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.94%
- CAS 番号: 1227692-67-2
- 分子式: C10H8Cl2N2O2
- 分子量:259.09
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| PC-12 | EC50 |
0.067 μM
Compound: 13
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Inhibition of SOD1 G93A mutant aggregation-induced cytotoxicity in rat PC12 cells incubated for 24 hrs and measured 72 hrs post compound dose by cytotoxicity protection assay
Inhibition of SOD1 G93A mutant aggregation-induced cytotoxicity in rat PC12 cells incubated for 24 hrs and measured 72 hrs post compound dose by cytotoxicity protection assay
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[PMID: 22191331] |
| PC-12 | EC50 |
0.067 μM
Compound: 5a
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Neuroprotective activity in rat PC12 cells expressing SOD1 G93A mutant assessed as increase in cell viability after 48 hrs by CalceinAM-based fluorescence assay
Neuroprotective activity in rat PC12 cells expressing SOD1 G93A mutant assessed as increase in cell viability after 48 hrs by CalceinAM-based fluorescence assay
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[PMID: 23021992] |
化学情報
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CAS 番号 1227692-67-2
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性状 Solid
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分子量 259.09
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分子式 C10H8Cl2N2O2
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Color Off-white to light brown
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SMILES
O=C1C=C(COC2=CC(Cl)=CC(Cl)=C2)NN1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (385.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
純度とドキュメンテーション
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データシート (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8597 mL | 19.2983 mL | 38.5966 mL | 96.4916 mL |
| 5 mM | 0.7719 mL | 3.8597 mL | 7.7193 mL | 19.2983 mL | |
| 10 mM | 0.3860 mL | 1.9298 mL | 3.8597 mL | 9.6492 mL | |
| 15 mM | 0.2573 mL | 1.2866 mL | 2.5731 mL | 6.4328 mL | |
| 20 mM | 0.1930 mL | 0.9649 mL | 1.9298 mL | 4.8246 mL | |
| 25 mM | 0.1544 mL | 0.7719 mL | 1.5439 mL | 3.8597 mL | |
| 30 mM | 0.1287 mL | 0.6433 mL | 1.2866 mL | 3.2164 mL | |
| 40 mM | 0.0965 mL | 0.4825 mL | 0.9649 mL | 2.4123 mL | |
| 50 mM | 0.0772 mL | 0.3860 mL | 0.7719 mL | 1.9298 mL | |
| 60 mM | 0.0643 mL | 0.3216 mL | 0.6433 mL | 1.6082 mL | |
| 80 mM | 0.0482 mL | 0.2412 mL | 0.4825 mL | 1.2061 mL | |
| 100 mM | 0.0386 mL | 0.1930 mL | 0.3860 mL | 0.9649 mL |
CMB-087229 関連分類
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