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製品番号: HY-D3255

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CTAP-1

CTAP-1 is a monovalent copper ion probe. CTAP-1 binds to monovalent copper Cu (I) to inhibit photoinduced electron transfer, thereby enhancing fluorescence emission. CTAP-1 produces copper-dependent perinuclear staining that colocalizes with mitochondria and the Golgi apparatus. CTAP-1 can be used for imaging Cu (I) pools, excitation/emission Wavelength: 365/480 nm.

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CAS 番号: 866534-64-7
分子式: C₃₂H₃₇N₃O₂S₄
分子量:623.92

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

CTAP-1 構造式

CAS 番号: 866534-64-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

CTAP-1 (10 μM; 50 min incubation) labels a perinuclear, copper-dependent labile copper pool in NIH 3T3 fibroblast cells, with fluorescence intensity increasing ~2- to 3-fold in copper-supplemented medium, and staining colocalizing with mitochondria and the Golgi apparatus^[1].
CTAP-1 (2 μM) fluorescence in copper-loaded NIH 3T3 fibroblast cells is reduced by ~50% upon treatment with the exogenous Cu⁺ chelator DTO, confirming the signal is copper-dependent^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

mouse NIH 3T3 fibroblast cells
Concentration:

10 μM (standard staining; immunofluorescence colocalization); 150 μM CuCl₂ (cell pre-culture)
Incubation Time:

50 min (37 °C, DMEM for standard staining; room temperature, PBS for immunofluorescence colocalization); 12 h (cell pre-culture with CuCl₂)
Result:

Produced a weak perinuclear staining pattern in basal medium.
Showed an ~2- to 3-fold stronger emission intensity in cells pre-cultured with 150 μM CuCl₂ for 12 h.
Colocalized staining pattern with subcellular locations of the Golgi apparatus and mitochondria.

化学情報

CAS 番号 866534-64-7
分子量 623.92
分子式 C₃₂H₃₇N₃O₂S₄
SMILES

[H][C@]1(CC(C2=CC=CC=C2)=NN1C3=CC=C(C(O)=O)C=C3)C(C=C4)=CC=C4N5CCSCCSCCSCCSCC5
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Yang L, et al. Imaging of the intracellular topography of copper with a fluorescent sensor and by synchrotron x-ray fluorescence microscopy. Proc Natl Acad Sci U S A. 2005;102(32):11179-11184. [Content Brief]

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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