Cy3 hydrazide
Cy3 hydrazide is a fluorescent probe for carbonylated proteins. Cy3 hydrazide covalently links to aldehyde or ketone residues on carbonylated proteins, reacts with protein carbonyl groups to form stable hydrazones, and enables fluorescent detection (Ex/Em = 555/570 nm).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 871514-32-8
- 分子式: C31H40N4O7S2
- 分子量:644.80
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Cy3 hydrazide (30 min (on ice labeling)) labels carbonylated proteins in mouse latissimus dorsi muscle extracts[1].
Cy3 hydrazide (5 mM; 30 min) enables multiplexed detection of carbonylated proteins in hydrogen peroxide-treated Saccharomyces cerevisiae W303-1A cell extracts via two-dimensional gel electrophoresis, but with spot shifting that reduces alignment with total protein stain[2].
Cy3 hydrazide (0.5-40 mM; 45 min-2 h) labels yeast tRNAPhe at dihydrouridine residues[3].
Cy3 hydrazide (40 mM; 2 h) labels reduced Escherichia coli tRNAArg[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 871514-32-8
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分子量 644.80
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分子式 C31H40N4O7S2
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SMILES
O=C(CCCCC[N+]1=C(/C=C/C=C2N(CC)C3=CC=C(S(=O)(O)=O)C=C3C\2(C)C)C(C)(C)C4=C1C=CC(S(=O)([O-])=O)=C4)NN
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Nina-Diogo A, et al. Synthesis of New Bodipy Hydrazide Fluorescent Probes for the Detection of Carbonylated Proteins Generated by Oxidative Stress. Chembiochem. 2024;25(19):e202400093. [Content Brief]
[2]. Tamarit J, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012;75(12):3778-3788. [Content Brief]
[3]. Pan D, et al. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009;15(2):346-354. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Cy3 hydrazide 関連分類
HY-172285 関連分類
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