DDO-2213
Based on 1 Customer Validation
DDO-2213 is an orally active and potent WDR5-MLL1 inhibitor, with an IC50 of 29 nM and a Kd value of 72.9 nM for the WDR5 protein. DDO-2213 selectively inhibits MLL (mixed lineage leukemia) histone methyltransferase activity and the proliferation of MLL translocation-harboring cells. DDO-2213 can be used for MLL fusion leukemia research.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.84%
- CAS 番号: 2378768-36-4
- 分子式: C24H27ClFN7O
- 分子量:483.97
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HUVEC | IC50 |
>100 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human HUVEC cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human HUVEC cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
| K562 | IC50 |
>100 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human K562 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human K562 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
| MOLM-13 | GI50 |
13.09 μM
Compound: DDO-2213
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Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
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[PMID: 34283608] |
| MOLM-13 | IC50 |
13.1 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human MOLM-13 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human MOLM-13 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
| MV4-11 | GI50 |
12.57 μM
Compound: DDO-2213
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Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
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[PMID: 34283608] |
| MV4-11 | IC50 |
12.6 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human MV4-11 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human MV4-11 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
化学情報
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CAS 番号 2378768-36-4
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性状 Solid
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分子量 483.97
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分子式 C24H27ClFN7O
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Color White to off-white
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SMILES
O=C(C1=CC=C(C2=CC(NC3=NC=NC(Cl)=C3N)=C(N4CCN(C)CC4)C=C2F)C=C1)N(C)C
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (206.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
純度とドキュメンテーション
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データシート (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0662 mL | 10.3312 mL | 20.6624 mL | 51.6561 mL |
| 5 mM | 0.4132 mL | 2.0662 mL | 4.1325 mL | 10.3312 mL | |
| 10 mM | 0.2066 mL | 1.0331 mL | 2.0662 mL | 5.1656 mL | |
| 15 mM | 0.1377 mL | 0.6887 mL | 1.3775 mL | 3.4437 mL | |
| 20 mM | 0.1033 mL | 0.5166 mL | 1.0331 mL | 2.5828 mL | |
| 25 mM | 0.0826 mL | 0.4132 mL | 0.8265 mL | 2.0662 mL | |
| 30 mM | 0.0689 mL | 0.3444 mL | 0.6887 mL | 1.7219 mL | |
| 40 mM | 0.0517 mL | 0.2583 mL | 0.5166 mL | 1.2914 mL | |
| 50 mM | 0.0413 mL | 0.2066 mL | 0.4132 mL | 1.0331 mL | |
| 60 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL | 0.8609 mL | |
| 80 mM | 0.0258 mL | 0.1291 mL | 0.2583 mL | 0.6457 mL | |
| 100 mM | 0.0207 mL | 0.1033 mL | 0.2066 mL | 0.5166 mL |
DDO-2213 関連分類
HY-153331 関連分類
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