DiFMUP
Based on 2 publication(s) in Google Scholar
DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader.
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- 純度: 98.86%
- CAS 番号: 214491-43-7
- 分子式: C10H7F2O6P
- 分子量:292.13
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 DiFMUP
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生物活性
DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 214491-43-7
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性状 Solid
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分子量 292.13
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分子式 C10H7F2O6P
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Color White to off-white
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SMILES
O=P(OC1=C(C(OC2=O)=C(C(C)=C2)C=C1F)F)(O)O
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別名
6,8-Difluoro-4-methylumbelliferyl phosphate
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Discovery of a Potent Fluorescence Polarization Probe for Identifying USP1 Allosteric Inhibitors. [Abstract]2026 Jul;13(39):e75350. PMID: 42017803 -
Eur J Med Chem
Discovery of novel substituted pyridine carboxamide derivatives as potent allosteric SHP2 inhibitors. [Abstract]2024 Dec 5:279:116830. PMID: 39303516
溶剤 & 溶解度
DMSO : 50 mg/mL (171.16 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Gee KR, et, al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem. 1999 Aug 15;273(1):41-8. [Content Brief]
[2]. Welte S, et, al. 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal Biochem. 2005 Mar 1;338(1):32-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4231 mL | 17.1157 mL | 34.2313 mL | 85.5783 mL |
| 5 mM | 0.6846 mL | 3.4231 mL | 6.8463 mL | 17.1157 mL | |
| 10 mM | 0.3423 mL | 1.7116 mL | 3.4231 mL | 8.5578 mL | |
| 15 mM | 0.2282 mL | 1.1410 mL | 2.2821 mL | 5.7052 mL | |
| 20 mM | 0.1712 mL | 0.8558 mL | 1.7116 mL | 4.2789 mL | |
| 25 mM | 0.1369 mL | 0.6846 mL | 1.3693 mL | 3.4231 mL | |
| 30 mM | 0.1141 mL | 0.5705 mL | 1.1410 mL | 2.8526 mL | |
| 40 mM | 0.0856 mL | 0.4279 mL | 0.8558 mL | 2.1395 mL | |
| 50 mM | 0.0685 mL | 0.3423 mL | 0.6846 mL | 1.7116 mL | |
| 60 mM | 0.0571 mL | 0.2853 mL | 0.5705 mL | 1.4263 mL | |
| 80 mM | 0.0428 mL | 0.2139 mL | 0.4279 mL | 1.0697 mL | |
| 100 mM | 0.0342 mL | 0.1712 mL | 0.3423 mL | 0.8558 mL |
DiFMUP 関連分類
HY-120166 関連分類
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