country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

製品番号: HY-D3186

Copy Link Print Information

HMRef-βGal

HMRef-βGal is a fluorescent probe and a substrate responsive to β-galactosidase (β-galactosidase) (Ex/Em=498 nm/505-600 nm). After being cleaved by β-galactosidase, HMRef-βGal triggers significant fluorescence enhancement via intramolecular spirocyclic function regulation. HMRef-βGal generates bright fluorescence in cancer cells with elevated β-galactosidase activity, enabling visualization of tiny peritoneal metastases in mouse models. HMRef-βGal exhibits low in vitro cytotoxicity and low acute in vivo toxicity in mice. HMRef-βGal can be used for preclinical fluorescence-guided diagnosis and cytoreductive surgery of peritoneal metastases, including compatibility with real-time naked-eye detection and endoscopic imaging, as well as for studies related to peritoneal metastases of ovarian cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 2163840-29-5
分子式: C₂₈H₂₆F₃NO₈
分子量:561.50

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

HMRef-βGal 構造式

CAS 番号: 2163840-29-5

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

HMRef-βGal (10 μM; 1 h) sensitively detects intracellular β-galactosidase activity in SHIN3, SKOV3, OVK18, OVCAR3, OVCAR4, OVCAR5, and OVCAR8 human ovarian cancer cell lines via live-cell confocal imaging (Ex/Em=498 nm/505-600 nm)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

HMRef-βGal (100 μM, 300 μL; intraperitoneal injection; single dose; imaging performed 5 min or 1 h post-administration) enables sensitive and specific visual imaging of tiny peritoneal ovarian cancer metastases with a diameter <1 mm in 7 distinct mouse models, and its signal brightness is sufficient to support naked-eye and endoscopic detection (imaging with a blue-green filter, Ex=470-510 nm; Em=530 nm)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/cAJcl-nu/nu nude mice with Ovarian cancer (female, 7-8 weeks old)^[1]
Dosage:

300 μL of 100 μM
Administration:

i.p.; single dose
Result:

Specifically visualized peritoneal metastases as small as <1 mm in diameter in all 7 ovarian cancer cell line models at 5 min post-administration.
Enhanced tumor nodule fluorescence sufficiently for naked-eye detection at 1 h post-administration.
Suppressed tumor nodule fluorescence when co-treated with a β-galactosidase inhibitor.
Successfully visualized metastases via real-time fluorescence endoscopy.

化学情報

CAS 番号 2163840-29-5
分子量 561.50
分子式 C₂₈H₂₆F₃NO₈
SMILES

FC(F)(F)CNC1=CC=C2C3(C4=CC=CC=C4CO3)C5=CC=C(C=C5OC2=C1)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Asanuma D, et al. Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo. Nat Commun. 2015;6:6463. Published 2015 Mar 13. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

お問い合わせ在庫あり

Other size

お問い合わせ