HW12
HW12 is a scrambled peptide control with no EGFR ligand activity. HW12 shows low cellular uptake levels in EGFR-positive cells. HW12 exerts weak growth inhibitory effects on pancreatic cancer. HW12 serves as a scrambled peptide control for EGFR-targeted GE11 (HY-P10128)-conjugated mixed micelles. HW12 can be used in pancreatic cancer research.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 875142-27-1
- 分子式: C72H89N19O17
- 分子量:1492.59
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
The HW12 peptide can be successfully conjugated with MAL-PEG-PCD via a thiol-maleimide addition reaction to form HW12-PEG-PCD, and this result is confirmed by 1H NMR analysis[1].
The cellular uptake of FC-labeled HW12-linked mixed micelles (12 h) by MIA PaCa-2 cells is significantly lower than that of GE11-conjugated mixed micelles, indicating that HW12 cannot mediate EGFR-specific cellular uptake[1].
HW12-linked mixed micelles (60-500 nM GEM; 72 h) exhibit lower cytotoxicity against MIA PaCa-2 cells than GE11-linked mixed micelles, with an IC50 of 126.58 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MIA PaCa-2 pancreatic cancer cells
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Concentration:HW12-linked mixed micelles with GEM concentrations of 60, 500 nM
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Incubation Time:72 h
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Result:Showed lower cytotoxicity than GE11-linked mixed micelles and unmodified micelles at all tested GEM concentrations.
Reached an IC50 value of 126.58 nM, which was 1.3-fold higher than the IC50 of GE11-linked mixed micelles (95.32 nM) and 1.1-fold higher than the IC50 of unmodified micelles (116.91 nM).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:athymic nude mice (6-8 week-old)[1]
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Dosage:40 mg/kg equivalent dose of gemcitabine
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Administration:i.v.; once weekly; 3 weeks
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Result:Showed minimal tumor growth inhibition compared to GE11-linked mixed micelles.
Resulted in significantly higher tumor bioluminescence, volume, and weight than in GE11-linked mixed micelle-treated mice.
Induced more Ki-67 staining, less cleaved caspase-3 staining, fewer necrotic tumor areas, less TUNEL-positive tumor and endothelial cell apoptosis, and higher tumor microvessel density compared to GE11-linked mixed micelles.
Caused no abnormal body weight changes.
Led to no pathological changes detected in major organs (heart, liver, kidney).
化学情報
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CAS 番号 875142-27-1
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分子量 1492.59
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分子式 C72H89N19O17
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
HW12 関連分類
HY-P11781 関連分類
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