Hydroxy-PEG3-DBCO
Hydroxy-PEG3-DBCO is a click chemistry reagent containing an azide group. Hydroxy-PEG3-DBCO is a PEG linker containing a DBCO moiety and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 2566404-76-8
- 分子式: C27H32N2O6
- 分子量:480.55
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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HY-140272
DBCO-PEG4-NHS esterPROTAC Linker -
HY-120770
DBCO-PEG4-MaleimideADC Linker -
HY-140279
DBCO-PEG4-alcoholPROTAC Linker -
HY-126885
DBCO-PEG5-NHS esterADC/PROTAC Linker -
HY-130396
DBCO-PEG4-C2-acidPROTAC Linker -
HY-140274
DBCO-PEG12-NHS esterPROTAC Linker -
HY-134714
DBCO-PEG3-aminePROTAC Linker -
HY-D1070
DBCO-PEG4-TAMRATARMA Dye -
HY-140280
DBCO-PEG1PROTAC Linker -
HY-140301
DBCO-PEG4-DesthiobiotinPROTAC Linker
生物活性
製品説明
化学情報
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CAS 番号 2566404-76-8
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分子量 480.55
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分子式 C27H32N2O6
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SMILES
O=C(CCC(N1C2=C(C#CC3=C(C1)C=CC=C3)C=CC=C2)=O)NCCOCCOCCOCCO
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Hydroxy-PEG3-DBCO 関連分類
HY-151822 関連分類
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