IM-54
Based on 1 publication(s) in Google Scholar
IM-54 is a selective inhibitor of oxidative stress-induced necrosis. IM-54 shows potent inhibitory activity against H2O2-induced necrosis. IM-54 acts as a potential cardioprotective agent and biological tool for investigating the molecular mechanisms of cell death.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.06%
- CAS 番号: 861891-50-1
- 分子式: C19H23N3O2
- 分子量:325.40
-
保管条件:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
-
Oxygen Sensing Compound Library
HY-L045
-
Anti-Cardiovascular Disease Compound Library
HY-L046
-
Anti-Parkinson's Disease Compound Library
HY-L085
-
Neurodegenerative Disease-related Compound Library
HY-L086
-
Cysteine Targeted Covalent Library
HY-L153
-
Cell Death Library
HY-L162
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
-
High-Throughput Bioactive Compound Library
HY-L205
-
Cell Death Inhibitor Library Mini
HY-L162M
-
Anti-Aging Compound Library
HY-L034
MedChemExpress（MCE）の使用を引用している文献 IM-54
More
生物活性
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| H9c2 | IC50 |
3.9 μM
Compound: IM-54
|
Cytoprotective activity against TBHP-induced necrosis in rat H9c2 cells incubated for 4 hrs by LDH release assay
Cytoprotective activity against TBHP-induced necrosis in rat H9c2 cells incubated for 4 hrs by LDH release assay
|
[PMID: 29541357] |
| HL-60 | IC50 |
0.25 μM
Compound: IM-54
|
Cytoprotective activity against H2O2-induced necrosis in human HL60 cells incubated for 1 hr followed by H2O2 stimulation by LDH release assay
Cytoprotective activity against H2O2-induced necrosis in human HL60 cells incubated for 1 hr followed by H2O2 stimulation by LDH release assay
|
[PMID: 29541357] |
IM-54 selectively inhibits oxidative stress-induced necrosis, but it does not inhibit apoptosis induced by various anticancer drugs or Fas ligand, or necroptosis[1].
IM-54 shows strong inhibition of H2O2-induced necrosis, with an IC50 of 0.25 μM in HL-60 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 861891-50-1
-
性状 Solid
-
分子量 325.40
-
分子式 C19H23N3O2
-
Color Light yellow to orange
-
SMILES
O=C(C(C1=CN(C)C2=C1C=CC=C2)=C3NCCCCC)N(C)C3=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (1)
-
Journal Impact Factor
-
Most Recent
溶剤 & 溶解度
DMSO : 50 mg/mL (153.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
-
データシート (276 KB)
-
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Kosuke Dodo, et al. Indolylmaleimide Derivative IM-17 Shows Cardioprotective Effects in Ischemia-Reperfusion Injury. ACS Med Chem Lett. 2018 Mar 8; 9(3): 182-187. [Content Brief]
[2]. Mikiko Sodeoka, et al. Development of selective inhibitors of necrosis. Chem Rec. 2010 Oct;10(5):308-14. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0731 mL | 15.3657 mL | 30.7314 mL | 76.8285 mL |
| 5 mM | 0.6146 mL | 3.0731 mL | 6.1463 mL | 15.3657 mL | |
| 10 mM | 0.3073 mL | 1.5366 mL | 3.0731 mL | 7.6829 mL | |
| 15 mM | 0.2049 mL | 1.0244 mL | 2.0488 mL | 5.1219 mL | |
| 20 mM | 0.1537 mL | 0.7683 mL | 1.5366 mL | 3.8414 mL | |
| 25 mM | 0.1229 mL | 0.6146 mL | 1.2293 mL | 3.0731 mL | |
| 30 mM | 0.1024 mL | 0.5122 mL | 1.0244 mL | 2.5610 mL | |
| 40 mM | 0.0768 mL | 0.3841 mL | 0.7683 mL | 1.9207 mL | |
| 50 mM | 0.0615 mL | 0.3073 mL | 0.6146 mL | 1.5366 mL | |
| 60 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2805 mL | |
| 80 mM | 0.0384 mL | 0.1921 mL | 0.3841 mL | 0.9604 mL | |
| 100 mM | 0.0307 mL | 0.1537 mL | 0.3073 mL | 0.7683 mL |
IM-54 関連分類
HY-108351 関連分類
Powered by Bioz