LAH4 TFA
Based on 1 Customer Validation
LAH4 TFA, an alpha-helix of the designed amphipathic peptide antibiotic, exhibits potent antimicrobial, nucleic acid transfection and cell penetration activities. LAH4 TFA possesses high plasmid DNA delivery capacities. LAH4 TFA has a strong affinity for anionic lipids found in the outer membrane of bacterial membranes.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.62%
- 分子式: C134H229F3N38O29
- 分子量:2893.55
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
LAH4 TFA is capable of complexing DNA, associating with the cell surfacemembrane and then, when enveloped within an endosome, disrupts the endosomal membrane as the pH drops[1].
LAH4 TFA possesses robust plasmid DNA transfection properties. Peptides of the LAH4 family are able to efficiently deliver siRNAs in vitro into a human cell line[2].
LAH4 TFA is found to mediate the intracellular delivery of both protein and nucleotide cargo and facilitate protein internalization using mechanisms involving endosomal acidification and processing through the proteasome pathway, leading to enhanced cross presentation of protein antigen by dendritic cells to CD8+ T cells. LAH4 TFA also improves the internalization of CpG, resulting in NF-kB activation, thus potentiating the adjuvant effect of CpG[3].
LAH4 TFA exhibits antibiotic activities against Escherichia coli and Bacillus subtilis. the peptide does not, however, lyse human red blood cells at bacteriocidal concentrations. The antibiotic activities of LAH4 TFA are 2 orders of magnitude more pronounced at pH 5 when compared with pH 7.5[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Solid
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分子量 2893.55
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分子式 C134H229F3N38O29
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Color White to off-white
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シーケンスの短縮
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
H2O : 100 mg/mL (34.56 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (8.64 mM); Clear solution; Need ultrasonic
純度とドキュメンテーション
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データシート (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Mason AJ, et al. The antibiotic and DNA-transfecting peptide LAH4 selectively associates with, and disorders, anionic lipids in mixed membranes. FASEB J. 2006;20(2):320-322. [Content Brief]
[2]. Langlet-Bertin B, et al. Design and evaluation of histidine-rich amphipathic peptides for siRNA delivery. Pharm Res. 2010;27(7):1426-1436. [Content Brief]
[3]. Zhang TT, et al. LAH4 enhances CD8+ T cell immunity of protein/peptide-based vaccines. Vaccine. 2012;30(4):784-793. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.3456 mL | 1.7280 mL | 3.4560 mL | 8.6399 mL |
| 5 mM | 0.0691 mL | 0.3456 mL | 0.6912 mL | 1.7280 mL | |
| 10 mM | 0.0346 mL | 0.1728 mL | 0.3456 mL | 0.8640 mL | |
| 15 mM | 0.0230 mL | 0.1152 mL | 0.2304 mL | 0.5760 mL | |
| 20 mM | 0.0173 mL | 0.0864 mL | 0.1728 mL | 0.4320 mL | |
| 25 mM | 0.0138 mL | 0.0691 mL | 0.1382 mL | 0.3456 mL | |
| 30 mM | 0.0115 mL | 0.0576 mL | 0.1152 mL | 0.2880 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
LAH4 TFA 関連分類
HY-P0311A 関連分類
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