MES hemisodium
Based on 4 publication(s) in Google Scholar
MES (2-Morpholinoethanesulphonic acid) hemisodium is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES hemisodium is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies.
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- 純度: 98.0%
- CAS 番号: 117961-21-4
- 分子式: C6H13NO4S.1/2Na
- 分子量:205.72
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保管条件:
RT, stored under nitrogen, away from moisture.
In solvent -80°C, 1 year , -20°C, 6 months
MedChemExpress(MCE)の使用を引用している文献 MES hemisodium
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生物活性
MES hemisodium is a zwitterionic buffer and can provide effective buffering capacity at around pH 6.15. The pKa value of MES hemisodium changes with temperature, so the change of its pKa value needs to be considered when using it at different temperatures[1].
MES hemisodium shows good buffering properties in biological experiments, exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), thereby can be used in studies involving metal ions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 117961-21-4
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性状 Solid
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分子量 205.72
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分子式 C6H13NO4S.1/2Na
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Color White to off-white
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SMILES
O=S(CCN1CCOCC1)([O-])=O.[Na+].[1/2]
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別名
2-Morpholinoethanesulphonic acid hemisodium
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
RT, stored under nitrogen, away from moisture
In solvent -80°C 1 year -20°C 6 months
Publications (4)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
A novel spherical GelMA-HAMA hydrogel encapsulating APET×2 polypeptide and CFIm25-targeting sgRNA for immune microenvironment modulation and nucleus pulposus regeneration in intervertebral discs. [Abstract]2024 Sep 12;22(1):556. PMID: 39267105 -
Phytomedicine
Da-Bu-Yin-Wan rescues cognitive deficits in aging and Alzheimer's disease models by Wnt/β-catenin-dependent restoration of lysosomal acidification. [Abstract]2026 Apr:153:157916. PMID: 41662806 -
J Colloid Interface Sci
Enhanced photostability and targeting ability of hollow mesoporous manganese-based nanocarriers for NIR-II fluorescence image-guided surgery and photothermal therapy. [Abstract]2025 Nov 15:698:138094. PMID: 40494115 -
Cell Biol Toxicol
IGFBP3 enhances adipose-derived stem cell function in soft tissue injury repair via ITGB1 and ERK pathway activation. [Abstract]2025 May 15;41(1):85. PMID: 40369223
溶剤 & 溶解度
H2O : ≥ 100 mg/mL (486.10 mM)
DMSO : 100 mg/mL (486.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. [Content Brief]
[2]. Tomoko Kagenishi, et al. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. Front Plant Sci. 2016 Feb 18;7:79. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.8610 mL | 24.3049 mL | 48.6098 mL | 121.5244 mL |
| 5 mM | 0.9722 mL | 4.8610 mL | 9.7220 mL | 24.3049 mL | |
| 10 mM | 0.4861 mL | 2.4305 mL | 4.8610 mL | 12.1524 mL | |
| 15 mM | 0.3241 mL | 1.6203 mL | 3.2407 mL | 8.1016 mL | |
| 20 mM | 0.2430 mL | 1.2152 mL | 2.4305 mL | 6.0762 mL | |
| 25 mM | 0.1944 mL | 0.9722 mL | 1.9444 mL | 4.8610 mL | |
| 30 mM | 0.1620 mL | 0.8102 mL | 1.6203 mL | 4.0508 mL | |
| 40 mM | 0.1215 mL | 0.6076 mL | 1.2152 mL | 3.0381 mL | |
| 50 mM | 0.0972 mL | 0.4861 mL | 0.9722 mL | 2.4305 mL | |
| 60 mM | 0.0810 mL | 0.4051 mL | 0.8102 mL | 2.0254 mL | |
| 80 mM | 0.0608 mL | 0.3038 mL | 0.6076 mL | 1.5191 mL | |
| 100 mM | 0.0486 mL | 0.2430 mL | 0.4861 mL | 1.2152 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.